Serosurveillance of Bovine Brucellosis Using RBPT and c-ELISA and Comparative Evaluation of Test Performance

Yuguda, Mugammad Umar; sundaram, S. Meenakshi; Lakshmanan, Gunaseelan; Ayyasamy, Elango; Purushothaman, Sridevi; Porteen, K.; Sanjeevi, Thiyagarajan

doi:10.20546/ijcmas.2019.808.067

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Overall, c-ELISA is the only test that picked seropositive ovines and caprines; no reactors were picked by either RBPT or i-ELISA. These results differ from those of others who reported seropositivity for ovine brucellosis in both tests, with some reporting i-ELISA to be more sensitive than RBPT or vise-versa ( Umar et al , 2019 ; Al-Marzooqi et al , 2022 ), while others, reported similar sensitivities for both tests ( Traoré et al , 2021 ). One of the problems of ELISAs performed on ovine serum is the high background reactivity obtained when testing sera from brucellosis-free animals ( De Bagüés et al , 1992 ).…”

Section: Discussioncontrasting

confidence: 99%

“…Overall, i-ELISA (14.4%; n = 40/278) picked significantly more seropositive bovine samples than both c-ELISA (5.04%; n = 14/278) and RBPT (2.2% n = 6/278) ( p < 0.05). The variations in the seropositivity in the three serological tests used in this study could be due to false positive and negative reactions which may occur with RBPT and i-ELISA, differences in sampling size, sensitivity and specificity of the employed serological tests, demography, and different clinical conditions of animals, and quality of the samples ( Ducrotoy et al , 2018 ; Umar et al , 2019 ; El-Diasty et al , 2021 ). In this study, i-ELISA picked 13 seropositive bovines that were not positive in either c-ELISA or RBPT.…”

Section: Discussionmentioning

confidence: 98%

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Seroprevalence and spatial distribution of livestock brucellosis using three serological tests in Kajiado County, Kenya

Odongo,

Bebora,

Gathumbi

et al. 2023

Open Vet J

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Background: Brucellosis is a serious zoonotic infection with a global socioeconomic impact on both the livestock industry and human health. In Kenya brucellosis is endemic but there is limited information on the true burden of the disease due to weak or peace-meal surveillance. The true burden and spread of animal brucellosis in Kajiado County is not known. Aim: The aim of the study was to determine the current seroprevalence and spatial distribution of livestock brucellosis in Kajiado County and also to compare the three serological tests, namely; RBPT, indirect ELISA, and competitive ELISA in the detection of seropositive animals. Methods: A cross-sectional study was undertaken in 5 Sub-counties and 13 wards, where a total of 782 serum samples from unvaccinated bovine (n = 278; 34 herds), ovine (n =256; 25 flocks), and caprine (n = 248; 28 flocks), were screened for anti-Brucella antibodies using Rose Bengal Plate test (RBPT), indirect ELISA (i-ELISA) and competitive ELISA (c-ELISA) tests, in parallel. Results: Overall animal seroprevalence was 6.91% (54/782); while that for bovine, ovine, and caprine was 18.35% (51/278), 0.78% (2/256) and 0.4% (1/248), respectively. Bovine seroprevalence was 2.2% (6/278), 14.4% (40/278), and 4.7% (13/278) in RBPT, i-ELISA, and c-ELISA tests, respectively; while ovine 0.78% (2/256) and caprine 0.4% (1/248) were positive only in c-ELISA. Bovine herd seropositivity was 67.65% (23/34), whereas ovine and caprine flock seropositivities were 8% (2/25) and 3.6% (1/28), respectively. Conclusion: The findings indicate a moderate seroprevalence of brucellosis in bovine, whilst that of ovine and caprine was low in Kajiado County. Indirect ELISA was found superior to both c-ELISA and RBPT in detecting bovine seropositive animals, while c-ELISA was superior to both RBPT and i-ELISA in detecting seropositive ovines and caprines. These results will contribute to baseline data for further study of Brucella infection and a starting point for the formulation of a strategy for the control of brucellosis in Kajiado County.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Seroprevalence and spatial distribution of livestock brucellosis using three serological tests in Kajiado County, Kenya

Odongo,

Bebora,

Gathumbi

et al. 2023

Open Vet J

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“…There is significant diversity of zoonoses around the world, but the World Health Organization (WHO) has selected this disease as one of the most serious “ignored zoonoses” in the world, since it has an especially substantial impact on poverty-stricken nations [ 13 ]. Brucella has been designated as a hazard group III pathogen by the WHO due to its ease of aerosol transmission [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

The Development of Diagnostic and Vaccine Strategies for Early Detection and Control of Human Brucellosis, Particularly in Endemic Areas

et al. 2023

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Brucellosis is considered one of the most serious zoonotic diseases worldwide. This disease affects both human and animal health, in addition to being one of the most widespread zoonotic illnesses in the Middle East and Northern Africa. Human brucellosis generally presents in a diverse and non-specific manner, making laboratory confirmation of the diagnosis critical to the patient’s recovery. A coordinated strategy for diagnosing and controlling brucellosis throughout the Middle East is required, as this disease cannot be known to occur without reliable microbiological, molecular, and epidemiological evidence. Consequently, the current review focuses on the current and emerging microbiological diagnostic tools for the early detection and control of human brucellosis. Laboratory assays such as culturing, serology, and molecular analysis can frequently be used to diagnose brucellosis. Although serological markers and nucleic acid amplification techniques are extremely sensitive, and extensive experience has been gained with these techniques in the laboratory diagnosis of brucellosis, a culture is still considered to be the “gold standard” due to the importance of this aspect of public health and clinical care. In endemic regions, however, serological tests remain the primary method of diagnosis due to their low cost, user-friendliness, and strong ability to provide a negative prediction, so they are commonly used. A nucleic acid amplification assay, which is highly sensitive, specific, and safe, is capable of enabling rapid disease diagnosis. Patients who have reportedly fully healed may continue to have positive molecular test results for a long time. Therefore, cultures and serological methods will continue to be the main tools for diagnosing and following up on human brucellosis for as long as no commercial tests or studies demonstrate adequate interlaboratory reproducibility. As there is no approved vaccine that prevents human brucellosis, vaccination-based control of animal brucellosis has become an important part of the management of human brucellosis. Over the past few decades, several studies have been conducted to develop Brucella vaccines, but the problem of controlling brucellosis in both humans and animals remains challenging. Therefore, this review also aims to present an updated overview of the different types of brucellosis vaccines that are currently available.

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Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India

Muthiah,

Singh,

Chhabra

et al. 2024

PLoS ONE

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Brucellosis and Q-fever are two highly contagious bacterial diseases with significant zoonotic potential and economic threats, yet they often remain underreported and neglected in low- and middle-income countries. The present study aimed to determine the seroprevalence of Brucellosis and Q-fever in water buffaloes in the Haryana state of India to implement effective preventive measures for disease control. The study covered all 22 districts of Haryana and involved 400 serum samples collected from female buffaloes belonging to two age groups and three distinct agro-climatic zones. The collected sera were tested using the Rose Bengal Plate agglutination test (RBPT) and commercial indirect enzyme linked immunosorbent assay (i-ELISA) for the presence of antibodies against smooth strains of Brucella spp. Additionally, the same serum samples were examined by i-ELISA for antibodies against Coxiella burnetii. The overall seroprevalence of brucellosis was observed as 8.25% (CI: 5.75–11.39) using the RBPT and 7.5% (CI: 5.12–10.53) by i-ELISA. The overall observed seroprevalence of Q-fever was 2.00% (CI: 0.87–3.90). No significant variation was seen in seropositivity of both diseases based on age and agroclimatic zones of the state. The findings of this study provide critical insights to farmers, agricultural organizations, veterinary services, and healthcare providers, facilitating more effective implementation of disease control measures.

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Serosurveillance of Bovine Brucellosis Using RBPT and c-ELISA and Comparative Evaluation of Test Performance

Cited by 6 publications

References 22 publications

Seroprevalence and spatial distribution of livestock brucellosis using three serological tests in Kajiado County, Kenya

Seroprevalence and spatial distribution of livestock brucellosis using three serological tests in Kajiado County, Kenya

The Development of Diagnostic and Vaccine Strategies for Early Detection and Control of Human Brucellosis, Particularly in Endemic Areas

Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India

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