Serosurvey of pathogenic hantaviruses among forestry workers in Hungary

Oldal, Miklós; Németh, Viktória; Madai, Mónika; Pintér, Réka; Kemenesi, Gábor; Dallos, Bianka; Kutas, Anna; Sebők, Judit; Horváth, Gyula; Bányai, Krisztiàn; Jakab, Ferenc

doi:10.2478/s13382-014-0308-2

Cited by 10 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rodent blood samples were screened for the presence of IgG antibodies against DOBV and PUUV by Western blot (WB) analysis. We applied recombinant DOBV and PUUV antigens, both produced in an Escherichia coli bacterial expression system, as previously described [17,18]. Nucleocapsid proteins (PUUV and DOBV) were loaded into the wells of Mini-PROTEAN ® TGX ™ Precast Gels (Bio-Rad Laboratories, Irvine, CA, USA).…”

Section: Serological Screening By Western Blot Analysismentioning

confidence: 99%

Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

Madai

Horváth

Herczeg

et al. 2021

Viruses

Self Cite

View full text Add to dashboard Cite

The natural hosts of Orthohantaviruses are rodents, soricomorphs and bats, and it is well known that they may cause serious or even fatal diseases among humans worldwide. The virus is persistent among animals and it is shed via urine, saliva and feces throughout the entirety of their lives. We aim to identify the effectiveness of hantavirus detection in rodent tissue samples and urine originating from naturally infected rodents. Initially, animals were trapped at five distinct locations throughout the Transdanubian region in Hungary. Lung, liver, kidney and urine samples were obtained from 163 deceased animals. All organs and urine were tested using nested reverse transcription polymerase chain reaction (nRT-PCR). Furthermore, sera were examined for IgG antibodies against Dobrava–Belgrade virus (DOBV) and Puumala virus (PUUV) by Western blot assay. IgG antibodies against hantaviruses and/or nucleic acid were detected in 25 (15.3%) cases. Among Apodemus, Myodes, and Microtus rodent species, DOBV, PUUV and Tula virus (TULV) were clearly identified. Amid the PCR-positive samples, the nucleic acid of the viruses was detected most effectively in the kidney (100%), while only 55% of screened lung tissues were positive. Interestingly, only three out of 20 rodent urine samples were positive when tested using nRT-PCR. Moreover, five rodents were seropositive without detectable virus nucleic acid in any of the tested organs.

show abstract

Section: Serological Screening By Western Blot Analysismentioning

confidence: 99%

Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

Madai

Horváth

Herczeg

et al. 2021

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, in the south-western regions of the United States, no antibodies against hantaviruses were found among a group of 140 forestry workers exposed to contact with rodents (Vitek et al 1996). Among forestry workers in Hungary, 4.6% of seropositve results were confirmed in the study group of 835 workers (for mix of serotypes Puumala and Dobrava) (Oldal et al 2014). In Switzerland, studies were carried out concerning the exposure to infection with hantaviruses among a group of 1,693 farmers, forestry workers and soldiers.…”

Section: Discussionmentioning

confidence: 77%

Exposure to infection with Hantavirus (serotype Dobrava/Hantaan) among forestry workers in Poland

Wróblewska-Łuczka

Chmielewska-Badora

Zwoliński

et al. 2017

Folia Forestalia Polonica

View full text Add to dashboard Cite

The hosts of Hantavirus are rodents, while they are transmitted to humans via aerosol, or by direct contact with them. The primary goal of the study was evaluation of the state of health of forestry workers by laboratory diagnosis of the diseases caused by Hantavirus Dobrava/Hantaan. The study covered a group of 820 randomly selected employees of the State Forests from 32 Forest Districts located in the area of the whole of Poland. The results of the study show that the largest number of positive results were registered in the Augustów Forest District -3 cases (10%), and in Piotrków Trybunalski -3 cases (11.5%). For the whole of Poland, 25 seropositive results were noted (3%) and 76 results were doubtful (9.3%). The results of the conducted study suggest the occurrence of Hantavirus Dobrava in large forest complexes on the territory of Poland. People residing and working in these areas are exposed to infection, and this exposure seems to be comparable with data from other countries.

show abstract

“…Because each rodent subfamily carries a phylogenetically different species of hantavirus, variations occur in the seroprevalence of endemic hantavirus species in each country and the infections caused by these species (16). Oldal et al (17) found that the prevalence of hantavirus IgG in forestry workers in Hungary was detected as 4.6% with the WB test. Engler et al (18) investigated the prevalence of hantavirus IgG in military personnel and blood donors in Switzerland and found the rate to be 0.3% in blood donors and 0.3% in the whole group via the WB method.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of hantavirus and Borreliaburgdorferi in Düzce (Turkey) forest villages and the relationship with sociodemographic features

AKAR¹

2019

Turk J Med Sci

View full text Add to dashboard Cite

Background/aim: Hantavirus and Borrelia burgdorferi are two zoonotic agents that pose a great risk especially for people living in forest areas. This study aimed to investigate the seroprevalence of hantavirus and B. burgdorferi in forest villages of Düzce and its relationship with sociodemographic features. Materials and methods:The presence of immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against hantavirus and B. burgdorferi in serum samples was investigated via enzyme-linked immunosorbent assay (ELISA). Hantavirus IgG and B. burgdorferi IgM and IgG positivity was then validated by western blot (WB) method.Results: During the analyses, 193 serum samples were tested. Eleven (6%) cases of hantavirus IgM was found positive by ELISA. Six (3%) cases of hantavirus IgG, 3 (2%) cases of B. burgdorferi IgM, and 12 (6%) cases of B. burgdorferi IgG were found positive by WB. Borrelia burgdorferi IgG positivity was found to be higher in the 46-70 age group and it was seen that the positivity was the highest among those who lived in mud-brick houses. Conclusion:This study was the first to determine the frequency of hantavirus in the study region and it includes current data for B. burgdorferi. Consequently, it is recommended that similar studies be carried out on rodents in all the regions at risk.

show abstract

Serosurvey of pathogenic hantaviruses among forestry workers in Hungary

Cited by 10 publications

References 30 publications

Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

Effectiveness Regarding Hantavirus Detection in Rodent Tissue Samples and Urine

Exposure to infection with Hantavirus (serotype Dobrava/Hantaan) among forestry workers in Poland

Seroprevalence of hantavirus and Borreliaburgdorferi in Düzce (Turkey) forest villages and the relationship with sociodemographic features

Contact Info

Product

Resources

About