Serotonergic Neurons Translate Taste Detection into Internal Nutrient Regulation

Zhang, Yao; Scott, Kristin

doi:10.1101/2021.05.13.444014

Cited by 4 publications

(6 citation statements)

References 75 publications

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“…Previous studies demonstrated that the serotonergic neurons in the lateral side of the gnathal ganglion facilitated the food ingestion of D. melanogaster larvae ( Schoofs et al, 2018 ). In addition to the internal regulation, the serotonergic neurons of the gnathal ganglion were also involved in mediating taste detection ( Yao and Scott, 2021 ). One class of serotonergic neurons in the gnathal ganglion responds to gustatory detection of sugars and the other class to gustatory detection of bitter compounds ( Yao and Scott, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the internal regulation, the serotonergic neurons of the gnathal ganglion were also involved in mediating taste detection ( Yao and Scott, 2021 ). One class of serotonergic neurons in the gnathal ganglion responds to gustatory detection of sugars and the other class to gustatory detection of bitter compounds ( Yao and Scott, 2021 ). As in other species, larval S. frugiperda possesses taste sensilla on the maxilla, responding to the stimuli sugar and bitter substances to regulate the feeding preference ( Hou et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

et al. 2022

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The fall armyworm Spodoptera frugiperda (S. frugiperda) (Lepidoptera: Noctuidae) is a worldwide, disruptive, agricultural pest species. The larvae of S. frugiperda feed on seedling, leave, and kernel of crops with chewing mouthparts, resulting in reduced crop yields. Serotonin is an important biogenic amine acting as a neural circuit modulator known to mediate lots of behaviors including feeding in insects. In order to explore the serotonergic neural network in the nervous system of larval S. frugiperda, we performed immunohistochemical experiments to examine the neuropil structure of the brain and the gnathal ganglion with antisynapsin and to examine their serotonergic neurons with antiserotonin serum. Our data show that the brain of larval S. frugiperda contains three neuromeres: the tritocerebrum, the deutocerebrum, and the protocerebrum. The gnathal ganglion also contains three neuromeres: the mandibular neuromere, the maxillary neuromere, and the labial neuromere. There are about 40 serotonergic neurons in the brain and about 24 serotonergic neurons in the gnathal ganglion. Most of these neurons are wide-field neurons giving off processes in several neuropils of the brain and the gnathal ganglion. Serotonergic neuron processes are mainly present in the protocerebrum. A pair of serotonergic neurons associated with the deutocerebrum has arborizations in the contralateral antennal lobe and bilateral superior lateral protocerebra. In the gnathal ganglion, the serotonergic neuron processes are also widespread throughout the neuropil and some process projections extend to the tritocerebrum. These findings on the serotonergic neuron network in larval S. frugiperda allow us to explore the important roles of serotonin in feeding and find a potential approach to modulate the feeding behavior of the gluttonous pest and reduce its damage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

et al. 2022

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“…Statistical analysis was carried out in Python using the SciPy package, version 1.7.3 (https://www.nature.com/articles/s41592-019-0686-2). deltaF/F0 images were created as described 40 .…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analysis was carried out in Python using the SciPy package, version 1.7.3 (https://www.nature.com/articles/s41592-019-0686-2). deltaF/F0 images were created as described 38 . In vivo functional connectivity experiments: In vivo sample preparation for calcium imaging was performed as described 29 .…”

Section: Methodsmentioning

confidence: 99%

Mating activates neuroendocrine pathways signaling hunger inDrosophilafemales

Laturney

Sterne

Scott

2022

Preprint

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Mated females reallocate resources to offspring production, causing changes in nutritional requirements and challenges to energy homeostasis. Although observed in most species, the neural and endocrine mechanisms that regulate the nutritional needs of mated females are not well understood. Here, we investigate the neural circuitry that regulates sugar appetite in matedDrosophila melanogasterfemales. During copulation, a male-derived sex peptide is transferred to females, silencing the mating status circuit to elicit many postmating behavioral changes1-3. We find that increased sucrose consumption is a postmated female behavior and show that it is mediated by the mating status circuit. We discovered that sexually dimorphic insulin receptor (Lgr3) neurons integrate mating status and nutritional state signals to adjust sucrose consumption. Lgr3+ cells receive inhibitory input from the mating status circuit via female specific pdC2 neurons. In mated females, the inhibition of Lgr3 cells from pdC2 is attenuated, transforming the mated signal into a long-term hunger signal that promotes sugar intake. Our results thus demonstrate that the mating circuit alters nutrient sensing centers in females to promote sugar consumption, providing a mechanism to increase intake in anticipation of the energetic costs associated with reproduction.

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“…TCA were performed similarly to those reported by Yao and Scott (2022). Two-four days old mated females were food-deprived for 22 hours and prepared for experiments as above (Fig.…”

Section: Behavioral Assaysmentioning

confidence: 99%

Taste adaptations associated with host-specialization in the specialistDrosophila sechellia

Reisenman

Wong

Vedagarbha

et al. 2022

Preprint

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Chemosensory-driven hostplant specialization is a major force mediating insect ecological adaptation and speciation.Drosophila sechellia, a species endemic to the Seychelles islands, feeds and oviposits onMorinda citrifoliaalmost exclusively. This fruit is harmless toD. sechelliabut toxic to otherDrosophilidae, including the closely related generalistsD. simulansandD. melanogaster, due to its high content of fatty acids. While several olfactory adaptations mediatingD. sechellia’spreference for its host have been uncovered, the role of taste has been much less examined. We found thatD. sechelliahas reduced taste and feeding aversion to bitter compounds and host fatty acids that are aversive toD. melanogasterandD. simulans. The loss of aversion to canavanine, coumarin, and fatty acids arose in theD. sechellialineage, as its sister speciesD. simulansshowed responses akin to those ofD. melanogaster. D. sechelliahas increased taste and feeding responses towardsM. citrifolia. These results are in line withD. sechellia’sloss of genes encoding bitter gustatory receptors (GRs) inD. melanogaster. We found that twoGRgenes which are lost inD. sechellia,GR39a.aandGR28b.a, influence the reduction of aversive responses to some bitter compounds. Also,D. sechelliahas increased appetite for a prominent host fatty acid compound that is toxic to its relatives. Our results support the hypothesis that changes in the taste system, specifically a reduction of sensitivity to bitter compounds that deter generalist ancestors, contribute to the specialization ofD. sechelliafor its host.Summary statementTaste specializations in the specialistDrosophila sechelliainclude a lineage-specific reduced sensitivity to bitter compounds associated with losses of gustatory receptors, and increased appetite for Noni and host fatty acids.

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Serotonergic Neurons Translate Taste Detection into Internal Nutrient Regulation

Cited by 4 publications

References 75 publications

Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

Serotonergic Neurons in the Brain and Gnathal Ganglion of Larval Spodoptera frugiperda

Mating activates neuroendocrine pathways signaling hunger inDrosophilafemales

Taste adaptations associated with host-specialization in the specialistDrosophila sechellia

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