Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host

Knecht, Leslie D.; O’Connor, Gregory; Mittal, Rahul; Liu, Xue Z.; Daftarian, Pirouz; Deo, Sapna K.; Daunert, Sylvia

doi:10.1016/j.ebiom.2016.05.037

Cited by 96 publications

(63 citation statements)

References 30 publications

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“…This bacterial communication is necessary for biofilm formation, swarming motility, induction of gene expression, exopolysaccharide production, and the exchange of virulence factors (Sauer et al, ; Liu et al, ; Abraham, ). Therefore, high serotonin levels resulted in increased P. aeruginosa pathogenicity by enhancing biofilm formation and elaboration of virulence factors, in vitro (Knecht et al, ). In a mouse model, we observed that animals treated with exogenous serotonin and infected with P. aeruginosa exhibited an increase in intestinal bacterial load and mortality compared to untreated animals.…”

Section: Serotoninmentioning

confidence: 99%

“…Recent studies from our laboratory showed that increased levels of serotonin can enhance quorum sensing in Pseudomonas aeruginosa both in vitro and in vivo (Knecht et al, 2016). Quorum sensing is the process by which bacteria in close proximity are able to communicate with one another through chemical signaling (Castillo-Ju arez et al, 2015).…”

Section: Journal Of Cellular Physiologymentioning

confidence: 99%

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Neurotransmitters: The Critical Modulators Regulating Gut–Brain Axis

Mittal

Debs

Patel

et al. 2017

Journal Cellular Physiology

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453

287

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Neurotransmitters including catecholamines and serotonin play a crucial role in maintaining homeostasis in the human body. Studies on these neurotransmitters mainly revolved around their role in the “fight or flight” response, transmitting signals across a chemical synapse and modulating blood flow throughout the body. However, recent research has demonstrated that neurotransmitters can play a significant role in the gastrointestinal (GI) physiology. Norepinephrine (NE), epinephrine (E), dopamine (DA), and serotonin have recently been a topic of interest because of their roles in the gut physiology and their potential roles in gastrointestinal and central nervous system pathophysiology. These neurotransmitters are able to regulate and control not only blood flow, but also affect gut motility, nutrient absorption, gastrointestinal innate immune system, and the microbiome. Furthermore, in pathological states such as inflammatory bowel disease (IBD) and Parkinson’s disease, the levels of these neurotransmitters are dysregulated, therefore causing a variety of gastrointestinal symptoms. Research in this field has shown that exogenous manipulation of catecholamine serum concentrations can help in decreasing symptomology and/or disease progression. In this review article, we discuss the current state-of-the-art research and literature regarding the role of neurotransmitters in regulation of normal gastrointestinal physiology, their impact on several disease processes, and novel work focused on the use of exogenous hormones and/or psychotropic medications to improve disease symptomology.

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Section: Serotoninmentioning

confidence: 99%

Section: Journal Of Cellular Physiologymentioning

confidence: 99%

Neurotransmitters: The Critical Modulators Regulating Gut–Brain Axis

Mittal

Debs

Patel

et al. 2017

Journal Cellular Physiology

Self Cite

453

287

View full text Add to dashboard Cite

show abstract

“…Host factors can crosstalk with microbiota via QS [14] and trigger AHL- and PQS-dependent QS communication (Fig. 1), for example neurotransmitter serotonin, estrogen steroid, and stress hormones [15, 16]. …”

Section: Qs Signaling Network In P Aeruginosamentioning

confidence: 99%

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of <b><i>Pseudomonas aeruginosa</i></b> Infections

Turkina

Vikström

2018

J Innate Immun

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Cell-to-cell signaling via small molecules is an essential process to coordinate behavior in single species within a community, and also across kingdoms. In this review, we discuss the quorum sensing (QS) systems used by the opportunistic pathogen Pseudomonas aeruginosa to sense bacterial population density and fitness, and regulate virulence, biofilm development, metabolite acquisition, and mammalian host defense. We also focus on the role of N-acylhomoserine lactone-dependent QS signaling in the modulation of innate immune responses connected together via calcium signaling, homeostasis, mitochondrial and cytoskeletal dynamics, and governing transcriptional and proteomic responses of host cells. A future perspective emphasizes the need for multidisciplinary efforts to bring current knowledge of QS into a more detailed understanding of the communication between bacteria and host, as well as into strategies to prevent and treat P. aeruginosa infections and reduce the rate of antibiotic resistance.

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“…Yet, in almost all organisms, elevated levels of 5-HT also signal stress (Chaouloff 2002;Chaouloff et al 1999;Deakin and Graeff 1991). Serotonin modulates quorum sensing and stimulates biofilm formation in Pseudomonas (Knecht et al 2016), a defense response of the bacterium, is synthesized and released upon exposure to abiotic stress in plants to promote abiotic stress tolerance (Erland et al 2016;Kaur et al 2015), promotes aversive behavior in C. elegans (Ooi and Prahlad 2017;Zhang et al 2005), and is released as one of the early responses to real or perceived threats by mammals (Chaouloff 2002;Chaouloff et al 1999;Deakin and Graeff 1991). Much of these contrary biological responses stimulated by 5-HT may have to do with the duration of exposure computed through feedback mechanisms that modulate the response of cells and animals to the presence of 5-HT at different timescales.…”

Section: ) (A) Vennmentioning

confidence: 99%

Global Transcriptome Changes That Accompany Alterations in Serotonin Levels inCaenorhabditis elegans

Cruz-Corchado¹,

Ooi²,

Das³

et al. 2020

G3 Genes|Genomes|Genetics

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Serotonin (5-hydroxytryptamine, 5-HT), is a phylogenetically ancient molecule best characterized as a neurotransmitter that modulates multiple aspects of mood and social cognition. The roles that 5-HT plays in normal and abnormal behavior are not fully understood but have been posited to be due to its common function as a ‘defense signal’. However, 5-HT levels also systemically impact cell physiology, modulating cell division, migration, apoptosis, mitochondrial biogenesis, cellular metabolism and differentiation. Whether these diverse cellular effects of 5-HT also share a common basis is unclear. C. elegans provides an ideal system to interrogate the systemic effects of 5-HT, since lacking a blood-brain barrier, 5-HT synthesized and released by neurons permeates the organism to modulate neuronal as well as non-neuronal cells throughout the body. Here we used RNA-Seq to characterize the systemic changes in gene expression that occur in C. elegans upon altering 5-HT levels, and compared the transcriptomes to published datasets. We find that an acute increase in 5-HT is accompanied by a global decrease in gene expression levels, upregulation of genes involved in stress pathways, changes that significantly correlate with the published transcriptomes of animals that have activated defense and immune responses, and an increase in levels of phosphorylated eukaryotic initiation factor, eIF2α. In 5-HT deficient animals lacking tryptophan hydroxylase (tph-1(mg280) II) there is a net increase in gene expression, with an overrepresentation of genes related to development and chromatin. Surprisingly, the transcriptomes of animals with acute increases in 5-HT levels, and 5-HT deficiency do not overlap with transcriptomes of mutants with whom they share striking physiological resemblance. These studies are the first to catalog systemic transcriptome changes that occur upon alterations in 5-HT levels. They further show that in C. elegans changes in gene expression upon altering 5-HT levels, and changes in physiology, are not directly correlated.

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Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host

Cited by 96 publications

References 30 publications

Neurotransmitters: The Critical Modulators Regulating Gut–Brain Axis

Neurotransmitters: The Critical Modulators Regulating Gut–Brain Axis

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of <b><i>Pseudomonas aeruginosa</i></b> Infections

Global Transcriptome Changes That Accompany Alterations in Serotonin Levels inCaenorhabditis elegans

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