Serotonin Depletion Affects Blood-Feeding but Not Host-Seeking Ability in Aedes triseriatus (Diptera: Culieidae)

Novak, Mark G.; Rowley, Wayne A.

doi:10.1093/jmedent/31.4.600

Cited by 52 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with the observed role of 5-HT in appetite modulation in numerous species, including mammals [Lucki, 1992] and insects [e.g. Dacks et al, 2003;Novak and Rowley, 1994;Novak et al, 1995]. While glutamate-and FMRFamidecontaining fibers are also present within the proventriculus, they are distinct from those that are serotonergic [Budnik et al, 1989].…”

Section: Introductionsupporting

confidence: 86%

A Trophic Role for Serotonin in the Development of a Simple Feeding Circuit

Neckameyer¹

2010

Dev Neurosci

View full text Add to dashboard Cite

Correct differentiation and positioning of individual synapses during development is fundamental to the normal function of neuronal circuits. While classical transmitters such as serotonin (5-HT) play a critical trophic role in neurogenesis in addition to their functions as transmitters in the mature nervous system, this process is not well understood. We used a simple model to assess both development and function of a specific behavioral circuit in the larval stage of the fruit fly (Drosophila melanogaster). We show that, as in all other species examined, the neurotransmitter actions of 5-HT depress feeding, and decreased neuronal 5-HT levels increase appetite. However, using transgenic tools, we show that constitutive knockdown of neuronal 5-HT synthesis to reduce 5-HT levels during central nervous system (CNS) development results in increased branching of the serotonergic fibers projecting to the gut, as well as increased size and number of varicosities along the neurite length. As larvae, these animals display decreased feeding rates relative to controls, and, when given exogenous 5-hydroxytryptophan, feeding is significantly enhanced. Late-stage wild-type embryos exposed to 5-HT to augment 5-HT levels during CNS development display, as mature larvae, a significant decrease in gut fiber branching and total varicosity number, as well as increased feeding and a hyposensitivity to the effects of 5-HT. Exposure of embryos unable to synthesize neuronal serotonin to 5-HT during late embryogenesis results in rescue of the feeding behavior and abnormalities in the 5-HT gut fiber architecture. These results demonstrate an inverse relationship between developmental 5-HT levels and complexity of the fiber architecture projecting to gut tissue, which results in a perturbed feeding pattern. We conclude that 5-HT is tightly regulated during CNS development to direct the normal architecture and mature function of this neural circuit.

show abstract

Section: Introductionsupporting

confidence: 86%

A Trophic Role for Serotonin in the Development of a Simple Feeding Circuit

Neckameyer¹

2010

Dev Neurosci

View full text Add to dashboard Cite

show abstract

“…Serotonin depresses feeding, whereas low neuronal serotonin levels increase appetite of Drosophila larvae (Neckameyer, 2010). In contrast, adults of the mosquito Aedes triseriatus respond to depletion of serotonin levels with a decrease in the amount of blood taken during feeding and in numbers of feeding episodes, but with no change in host-seeking behavior, suggesting that serotonin stimulates feeding (Novak and Rowley, 1994). The apparent difference in the roles of serotonin on appetite of larval and adult mosquitoes merits further study.…”

Section: Effects Of Paroxetine On Behaviormentioning

confidence: 98%

“…Serotonin also modulates insect feeding behavior. Serotonergic systems inhibit feeding in the locust, the ant, adults of two species of fly, an aphid, a cockroach and larval Drosophila (Dacks et al, 2003;Kaufmann et al, 2004;Falibene et al, 2012;Neckameyer, 2010), although serotonin appears to stimulate feeding by the adult mosquito Aedes triseriatus (Novak and Rowley, 1994). As in vertebrates, serotonergic pathways inhibit appetite and feeding behavior in insects (Gillette, 2006;Halford et al, 2007).…”

Section: Serotonin and Behaviormentioning

confidence: 99%

Modulation of appetite and feeding behavior of the larval mosquito Aedes aegypti (L.) by the serotonin-selective reuptake inhibitor paroxetine: shifts between distinct feeding modes and the influence of feeding status.

Kinney

Panting

Clark

2013

Journal of Experimental Biology

View full text Add to dashboard Cite

The effects of the serotonin-selective reuptake inhibitor paroxetine (2×10 -5 mol l -1) on behavior of the larval mosquito Aedes aegypti are described. Four discrete behavioral states dominate larval behavior: wriggling, two distinct types of feeding, and quiescence. Feeding behaviors consist of foraging along the bottom of the container (substrate browsing), and stationary filter feeding while suspended from the surface film. Fed larvae respond to paroxetine with increased wriggling, and reductions in both feeding behaviors. In contrast, food-deprived larvae treated with paroxetine show no change in the proportion of time spent wriggling or feeding, but shift from stationary filter feeding to substrate browsing. Thus, actions of paroxetine in fed larvae are consistent with suppression of appetite and stimulation of wriggling, whereas paroxetine causes fooddeprived larvae to switch from one feeding behavior to another. Further analysis of unfed larvae revealed that paroxetine decreased the power stroke frequency during wriggling locomotion, but had no effect on the swimming velocity during either wriggling or substrate browsing. These data suggest that: (1) serotonergic pathways may trigger shifts between distinct behaviors by actions on higher level (brain) integrating centers where behaviors such as feeding and locomotion are coordinated; (2) these centers in fed and fooddeprived larvae respond differently to serotonergic stimulation suggesting sensory feedback from feeding status; and (3) serotonergic pathways also modulate central pattern generators of the nerve cord where the bursts of action potentials originate that drive the rhythmic muscle contractions of wriggling.

show abstract

“…This process stimulates water absorption by the midgut and urine formation by the Malpighian tubules (Te Brugge et al, 2009). Indirect evidence suggests that serotonin release also occurs in mosquitoes during the hematophagic process (Novak and Rowley, 1994;Novak et al, 1995;Clark et al, 2009). Even among the non-hematophagous larvae of mosquitoes, studies have shown that serotonin acts on the midgut to increase the transepithelial voltage and, consequently, the rate of alkalization (Onken et al, 2008;Onken and Moffet, 2009).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of pH control in the midgut ofLutzomyia longipalpis: roles for ingested molecules and hormones

Santos

Nunes

Pereira

et al. 2011

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYControl of the midgut pH in Lutzomyia longipalpis enables the insect's digestive system to deal with different types of diet. Phlebotomines must be able to suddenly change from a condition adequate to process a sugar diet to one required to digest blood. Prior to blood ingestion, the pH in the midgut is maintained at ~6 via an efficient mechanism. In the abdominal midgut, alkalization to a pH of ~8 occurs as a consequence of the loss of CO 2 from blood (CO 2 volatilization) and by a second mechanism that is not yet characterized. The present study aimed to characterize the primary stimuli, present in the blood, that are responsible for shutting down the mechanism that maintains a pH of 6 and switching on that responsible for alkalization. Our results show that any ingested protein could induce alkalization. Free amino acids, at the concentrations found in blood, were ineffective at inducing alkalization, although higher concentrations of amino acids were able to induce alkalization. Aqueous extracts of midgut tissue containing putative hormones from intestinal endocrine cells slightly alkalized the midgut lumen when applied to dissected intestines, as did hemolymph collected from blood-fed females. Serotonin, a hormone that is possibly released in the hemolymph after hematophagy commences, was ineffective at promoting alkalization. The carbonic anhydrase (CA) enzyme seems to be involved in alkalizing the midgut, as co-ingestion of acetazolamide (a CA inhibitor) with proteins impaired alkalization efficiency. A general model of alkalization control is presented.

show abstract

Serotonin Depletion Affects Blood-Feeding but Not Host-Seeking Ability in Aedes triseriatus (Diptera: Culieidae)

Cited by 52 publications

References 0 publications

A Trophic Role for Serotonin in the Development of a Simple Feeding Circuit

A Trophic Role for Serotonin in the Development of a Simple Feeding Circuit

Modulation of appetite and feeding behavior of the larval mosquito Aedes aegypti (L.) by the serotonin-selective reuptake inhibitor paroxetine: shifts between distinct feeding modes and the influence of feeding status.

Mechanisms of pH control in the midgut ofLutzomyia longipalpis: roles for ingested molecules and hormones

Contact Info

Product

Resources

About