Serotonin Excites Neurons in the Human Submucous Plexus via 5-HT3 Receptors

Michel, Klaus; Zeller, Florian; Langer, Rupert; Nekarda, H.; Krüger, D.; Dover, Terri J.; Brady, Catherine; Barnes, Nicholas M.; Schemann, Michael

doi:10.1053/j.gastro.2005.02.005

Cited by 82 publications

(114 citation statements)

References 35 publications

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“…1) was almost 50% less than the total occurrences of HTR3A and agrees with previous findings where lower levels but no distinct patterns of expression were observed. 23,25,27 Interestingly, the expression and occurrence of the 5-HT 3 receptor C subunit was similar to HTR3A while HTR3E was predominantly restricted to the mucosa confirming prior observations in the colon, small intestine and stomach. 22,23,25,26,38,49 The mucosa plays a role in fluid transport, while the muscular layers are involved with motility so it is possible that physiological alterations in the different colon tissue layers may result in selective colon disease or dysfunction.…”

Section: Discussionsupporting

confidence: 85%

“…[19][20][21] All of the human 5-HT 3 receptor subunit genes are expressed in the gastrointestinal tract. [22][23][24][25][26] Immunohistochemistry studies have pinpointed co-expression of A, B, C, D, and E subunits in enterocytes and also the nerves of the myenteric plexus 24,27 indicating that heteromeric 5-HT 3 receptors are likely to form in the human colon. Although 5-HT 4 and 5-HT 7 receptor splice variants have been identified in separate studies in the small intestine and colon, 22,[28][29][30] no study has looked at their tissue distribution along the colon.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Yaakob

Chinkwo

Chetty

et al. 2015

J Neurogastroenterol Motil

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Background/AimsSeveral disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT 3 and 5-HT 4 receptors are clinically relevant. The aim was to examine the distribution of 5-HT 3 , 5-HT 4 , and 5-HT 7 receptors in the normal human colon and how this is associated with receptor interacting chaperone 3, G protein coupled receptor kinases, and protein LIN-7 homologs to extend previous observations limited to the sigmoid colon or the upper intestine. MethodsSamples from ascending, transverse, descending, and sigmoid human colon were dissected into 3 separate layers (mucosa, longitudinal, and circular muscles) and ileum samples were dissected into mucosa and muscle layers (n = 20). Complementary DNA was synthesized by reverse transcription from extracted RNA and expression was determined by quantitative or end point polymerase chain reaction. ResultsThe 5-HT3 receptor subunits were found in all tissues throughout the colon and ileum. The A subunit was detected in all samples and the C subunit was expressed at similar levels while the B subunit was expressed at lower levels and less frequently. The 5-HT3 receptor E subunit was mainly found in the mucosa layers. All splice variants of the 5-HT4 and 5-HT7 receptors were expressed throughout the colon although the 5-HT4 receptor d, g, and i variants were expressed less often. ConclusionsThe major differences in 5-HT receptor distribution within the human colon are in relation to the mucosa and muscular tissue layers where the 5-HT 3 receptor E subunit is predominantly found in the mucosal layer which may be of therapeutic relevance.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Yaakob

Chinkwo

Chetty

et al. 2015

J Neurogastroenterol Motil

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“…Activation of 5-HT 3 receptors stimulates anion secretion 11 and motility, an effect blocked by 5-HT 3 receptor…”

Section: Methodsmentioning

confidence: 99%

Effects of a 5‐HT₃ antagonist, ondansetron, on fasting and postprandial small bowel water content assessed by magnetic resonance imaging

Marciani

Wright

Foley

et al. 2010

Aliment Pharmacol Ther

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SUMMARYBackground 5-HT 3 antagonists have been shown to be effective in relieving the symptoms of irritable bowel syndrome with diarrhoea (IBS-D). Using a recently validated magnetic resonance imaging (MRI) method, we have demonstrated reduced fasting small bowel water content (SBWC) in IBS-D associated with accelerated small bowel transit. We hypothesized that slowing of transit with ondansetron would lead to an increase in SBWC by inhibiting fasting motility.

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“…These neurons detect variations of the intestinal lumen and in the mucosal homeostasis, presenting specific receptors for serotonin (5-HT) secreted by enterochromaphin cells of the mucosa, and performing an important role in ENS afference (Michel et al, 2005).…”

Section: Nadph-dmentioning

confidence: 99%

Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

et al. 2016

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SUMMARY:Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the submucosal plexus of adult rats are mainly organized in ganglia with varied sized and shapes. Giemsa technique stained 243.93 ± 7.68 neurons per mm 2 . Regarding the total population stained by Giemsa, NADH-diaphorase positive (139.09 ± 11.14/mm 2 ) neurons represented 57 % and NADPH-diaphorase positive (18.17 ± 0.28/mm 2 ) represented 7.5 %. The area of the cell body was bigger in nitrergic neurons (412.29 ± 150.22) than in the ones stained by Giemsa (254.71 ± 63.32) and NADH-diaphorase positive (243.98 ± 123.82).

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Serotonin Excites Neurons in the Human Submucous Plexus via 5-HT3 Receptors

Cited by 82 publications

References 35 publications

Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Effects of a 5‐HT₃ antagonist, ondansetron, on fasting and postprandial small bowel water content assessed by magnetic resonance imaging

Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

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Serotonin Excites Neurons in the Human Submucous Plexus via 5-HT3 Receptors

Cited by 82 publications

References 35 publications

Distribution of 5-HT3, 5-HT4, and 5-HT7Receptors Along the Human Colon

Distribution of 5-HT3, 5-HT4, and 5-HT7Receptors Along the Human Colon

Effects of a 5‐HT3 antagonist, ondansetron, on fasting and postprandial small bowel water content assessed by magnetic resonance imaging

Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

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Product

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Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Distribution of 5-HT₃, 5-HT₄, and 5-HT₇Receptors Along the Human Colon

Effects of a 5‐HT₃ antagonist, ondansetron, on fasting and postprandial small bowel water content assessed by magnetic resonance imaging