Serotonin immunoreactivity in the nervous system of the dragonfly nymph

Abstract

Serotonin-like immunoreactivity was mapped using an antiserotonin antibody in wholemounts of the ventral nerve cord from dragonfly nymphs (Epitheca sp. and Pachydiplax longipennis). In both species, an immunoreactive cell ventral to each connective tract and an immunoreactive median cell cluster on the ganglion ventral surface were found in the unfused abdominal ganglia. Axon(s) from the median cell cluster branch in the anterior unpaired median nerve. Posterolaterally, in all of the ganglia examined, two or more intensely immunoreactive, bilaterally symmetric pairs of neurons were seen. Comparison of these posterolateral neurons, which appear to be serially homologous, with similar antiserotonin immunoreactive neurons described in other insects suggests that these neuron pairs may have cross-species homology as well.

Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda)

“…The distribution of serotonin has been extensively mapped in a number of arthropods (e.g., Myhrberg et al 1979;Beltz and Kravitz 1983;Bishop and O'Shea 1983;Elofsson 1983Elofsson , 1992Cournil et al 1984;Klemm et al 1984;Nässel and Elekes 1984;Schürmann and Klemm 1984;Nässel and Elekes 1985;Longley and Longley 1986;Siwicki et al 1987;Sandeman et al 1988;Dubbels and Elofsson 1989;Homberg and Hildebrand 1989;Katz et al 1989;Beltz et al 1990;Real and Czternasty 1990;Salecker and Distler 1990;Lundell and Hirsh 1994;Thompson et al 1994;Christie et al 1995;Harzsch and Dawirs 1995;Schürmann et al 1995;Würden and Homberg 1995;Bicker 1999;Callaway and Stuart 1999;Hörner 1999;Leitinger et al 1999;Tierney et al 1999;Wegerhoff 1999;Ignell 2001;Homberg 2002;Moreau et al 2002;Harzsch 2004;Settembrini and Villar 2004;Fu et al 2005;Gallus et al 2005;Barthelemy et al 2006;…”

Immunohistological localization of serotonin in the CNS and feeding system of the stable fly STOMOXYS CALCITRANS L. (Diptera: Muscidae)

“…5-HT immunoreactive (IR) neurons have been located in the central nervous system (CNS) and peripheral nervous system of many insect species, including the horse fly (Tabanus nigrovittatus) (Haselton et al, 2006), the dragonfly (Epitheca spp. and Pachydiplax longipennis) (Longley and Longley, 1986), the yellow fever mosquito (Aedes aegypti) and the African malaria mosquito (Anopheles gambiae) (Moffett and Moffet, 2005;Siju et al, 2008). 5-HT affects feeding preference, meal size, and feeding-related activities in the black blow fly, mosquitoes, the locust, the flesh fly, and the Rhodnius bug (Long and Murdock, 1983;Novak and Rowley, 1994;Novak et al, 1995;Dacks et al, 2003;Molaei and Lange, 2003;Orchard, 2006).…”

Serotonergic neurons in the ventral nerve cord of Chilopoda – a mandibulate pattern of individually identifiable neurons

“…However, since the availability of specific antibodies against 5HT [2, 3], approaches for the identification of these neurons has mainly focused on Tetraconata (Crustacea and Hexapoda), starting with investigations in lobsters [4] and cockroaches [5]. The first comparative studies pointing out striking morphological similarities of certain neuron types were initially restricted to winged insects, such as dragonflies, cockroaches, grasshoppers, and flies [6], inferring homologies at single cell level [7]. The homology of individually identifiable 5HT-ir neurons in insects was strongly supported by developmental studies in grasshopper and the fruit fly, demonstrating that these neurons are derived from the same neuronal progenitor cell, the neuroblast 7-3 [5, 8, 9].…”