Serotonin-induced brain glycogenolysis in rainbow trout (<i>Oncorhynchus mykiss</i>)

Maceira, Jorge José Pérez; Mancebo, M.J.; Aldegunde, M.

doi:10.1242/jeb.070649

Cited by 12 publications

(2 citation statements)

References 81 publications

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“…Results are expressed as nmol/min per gram of wet tissue. Hepatic glycogen phosphorylase activity assay was determined as previously described (33). Results are expressed as a percentage of activity.…”

Section: Enzymatic Activitiesmentioning

confidence: 99%

Hypothalamic Apelin/Reactive Oxygen Species Signaling Controls Hepatic Glucose Metabolism in the Onset of Diabetes

Drougard

Duparc

Brénachot

et al. 2014

Antioxidants & Redox Signaling

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Aims: We have previously demonstrated that central apelin is implicated in the control of peripheral glycemia, and its action depends on nutritional (fast versus fed) and physiological (normal versus diabetic) states. An intracerebroventricular (icv) injection of a high dose of apelin, similar to that observed in obese/diabetic mice, increase fasted glycemia, suggesting (i) that apelin contributes to the establishment of a diabetic state, and (ii) the existence of a hypothalamic to liver axis. Using pharmacological, genetic, and nutritional approaches, we aim at unraveling this system of regulation by identifying the hypothalamic molecular actors that trigger the apelin effect on liver glucose metabolism and glycemia. Results: We show that icv apelin injection stimulates liver glycogenolysis and gluconeogenesis via an over-activation of the sympathetic nervous system (SNS), leading to fasted hyperglycemia. The effect of central apelin on liver function is dependent of an increased production of hypothalamic reactive oxygen species (ROS). These data are strengthened by experiments using lentiviral vectormediated over-expression of apelin in hypothalamus of mice that present over-activation of SNS associated to an increase in hepatic glucose production. Finally, we report that mice fed a high-fat diet present major alterations of hypothalamic apelin/ROS signaling, leading to activation of glycogenolysis. Innovation/Conclusion: These data bring compelling evidence that hypothalamic apelin is one master switch that participates in the onset of diabetes by directly acting on liver function. Our data support the idea that hypothalamic apelin is a new potential therapeutic target to treat diabetes. Antioxid. Redox Signal. 20, 557-573.

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Section: Enzymatic Activitiesmentioning

confidence: 99%

Hypothalamic Apelin/Reactive Oxygen Species Signaling Controls Hepatic Glucose Metabolism in the Onset of Diabetes

Drougard

Duparc

Brénachot

et al. 2014

Antioxidants & Redox Signaling

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“…Indeed, in gulf toadfish, hypoxia exposure during acute intraperitoneal treatment of 20 or 50 µg/g of fluoxetine have been shown to accentuate oxyconformation (i.e., decreased regulation index) and reduce the ventilatory response, respectively (Amador et al, 2018;Panlilio et al, 2016). Serotonin also regulates glycogenolysis in the brain of rainbow trout (Pérez-Maceira et al, 2012), so SSRI exposure could have also contributed to the effects of hypoxia and wastewater exposure on glycogen levels that were observed here. β-blockers such as propranolol can affect heart rate in zebrafish larvae at waterborne exposure concentrations of 0.05 mM (Fraysse et al, 2006), can disrupt oxygen chemoreception by neuroepithelial cells in the gills of rainbow trout at concentrations of 1-2 mM in the gill perfusate (Burleson and Milsom, 1990), and can disrupt blood-flow distribution through the gills of rainbow trout at 0.1 mM in gill perfusate (Payan and Girard, 1977).…”

Section: Wastewater Exposure Disrupts Responses To Chronic Hypoxiamentioning

confidence: 58%

Exposure to wastewater effluent disrupts hypoxia responses in killifish (Fundulus heteroclitus)

Lau

Mehdi

Bragg

et al. 2021

Environmental Pollution

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Food intake inhibition in rainbow trout induced by activation of serotonin 5-HT2C receptors is associated with increases in POMC, CART and CRF mRNA abundance in hypothalamus

et al. 2016

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In rainbow trout, the food intake inhibition induced by serotonin occurs through 5-HT2C and 5-HT1A receptors, though the mechanisms involved are still unknown. Therefore, we assessed if a direct stimulation of 5-HT2C and 5-HT1A serotonin receptors (resulting in decreased food intake in rainbow trout), affects gene expression of neuropeptides involved in the control of food intake, such as pro-opiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript (CART), corticotrophin releasing factor (CRF), and agouti-related peptide (AgRP). In a first set of experiments, the injection of the 5-HT2C receptor agonists MK212 (60 μg kg(-1) icv) and WAY 161503 (1 mg kg(-1) ip), and of the 5-HT1A receptor agonist 8-OH-DPAT (1 mg kg(-1) ip and 30 μg kg(-1) icv) induced food intake inhibition. In a second set of experiments, we observed that the injection of MK212 or WAY 161503 (1 and 3 mg kg(-1)) significantly increased hypothalamic POMC mRNA abundance. CART mRNA abundance in hypothalamus was enhanced by treatment with MK212 and unaffected by WAY 161503. The administration of the 5-HT1A receptor agonist 8-OH-DPAT did not induce any significant variation in the hypothalamic POMC or CART mRNA levels. CRF mRNA abundance was only affected by MK212 that increased hypothalamic values. Finally, hypothalamic AgRP mRNA abundance was only evaluated with the agonist 5-HT2C MK212 resulting in no significant effects. The results show that the reduction in food intake mediated by 5-HT2C receptors is associated with increases in hypothalamic POMC, CART and CRF mRNA abundance.

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Serotonin-induced brain glycogenolysis in rainbow trout (Oncorhynchus mykiss)

Abstract: SUMMARY

Cited by 12 publications

References 81 publications

Hypothalamic Apelin/Reactive Oxygen Species Signaling Controls Hepatic Glucose Metabolism in the Onset of Diabetes

Hypothalamic Apelin/Reactive Oxygen Species Signaling Controls Hepatic Glucose Metabolism in the Onset of Diabetes

Exposure to wastewater effluent disrupts hypoxia responses in killifish (Fundulus heteroclitus)

Food intake inhibition in rainbow trout induced by activation of serotonin 5-HT2C receptors is associated with increases in POMC, CART and CRF mRNA abundance in hypothalamus

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