Serotonin inhibits high-threshold Ca2+ channel currents in capsaicin-sensitive acutely isolated adult rat DRG neurons

Mar, Lucinda P. Del; Cardenas, Carla G.; Scroggs, Reese S.

doi:10.1152/jn.1994.72.5.2551

Cited by 27 publications

(6 citation statements)

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“…In the present experiments, 5‐CT showed a similar depressant effect on the responses to NMDA but a stronger depressant effect on the DR‐EPSP than 5‐HT. As 5‐CT is a powerful agonist at 5‐HT 1 receptors it is possible that it acted on slowly conducting primary afferents to decrease the release of sensory transmitters via the inhibition of calcium entry at the terminals or other mechanisms (Del Mar et al . 1994;Hori et al .…”

Section: Resultsmentioning

confidence: 99%

“…Different effects of 5‐HT have been reported on presynaptic afferent terminals as well as on postsynaptic dorsal horn neurons. At a presynaptic level 5‐HT has been shown to cause depolarization and hyperpolarization of primary afferents (Hentall & Fields 1983;Carstens et al 1981), inhibition of calcium currents in dorsal root ganglion neurons (Del Mar et al . 1994;Cardenas et al .…”

Section: Introductionmentioning

confidence: 99%

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Serotonergic modulation of the responses to excitatory amino acids of rat dorsal horn neurons in vitro: implications for somatosensory transmission

López-García

1998

Eur J of Neuroscience

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Serotonin (5-HT) is one of the major transmitters involved in supraspinal control of somatic sensation and nociception. The aim of the present study was to investigate if the 5-HT-induced modulation of sensory transmission in the dorsal horn could be due to regulation of neuronal responses to excitatory amino acids. Experiments were performed in an in vitro preparation of the young rat spinal cord. Responses to dorsal root stimulation (DR-EPSP) and to droplet application of N-methyl-D-aspartic acid (NMDA) and alpha-amino-2,3-dihydro-5-methyl-3-oxo-4-isoxazolepropanoic acid (AMPA) were obtained by means of intracellular recordings of dorsal horn neurons. Bath applications of 5-HT (50 microM) generally caused reductions in amplitude and integrated area of DR-EPSPs and of responses to NMDA but the responses to AMPA were unaltered. A linear correlation was found between the effects of 5-HT on the DR-EPSP and on the NMDA response measured as percentage change in amplitude (r2 = 0.45; P < or = 0.01) and integrated area (r2 = 0.77; P < or = 0.001). The NMDA receptor antagonist d-AP5 (50 microM) completely abolished NMDA responses and caused a depression of the DR-EPSP similar to that of 5-HT. The 5-HT1 receptor agonist 5-carboxamidotryptamine (5-CT; 1 microM) mimicked the depressant effects of 5-HT but had a stronger depressant action on the DR-EPSP than 5-HT. The depression of NMDA responses induced by 5-HT and 5-CT was tetrodotoxin (1 microM) resistant. It is concluded that 5-HT-induced depression of NMDA responses explains partially the depressant action of 5-HT on dorsal horn synaptic transmission activating a postsynaptic site sensitive to 5-CT. The possible activation of coadjuvant mechanisms is discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serotonergic modulation of the responses to excitatory amino acids of rat dorsal horn neurons in vitro: implications for somatosensory transmission

López-García

1998

Eur J of Neuroscience

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“…1997; hypothalamic neurons, Koike et al . 1994; dorsal root ganglion neurons, Del Mar et al . 1994; cortical pyramidal neurons, Foehring 1996; hypoglossal motoneurons, Bayliss et al .…”

Section: Discussionmentioning

confidence: 99%

The serotonin inhibition of high‐voltage‐activated calcium currents is relieved by action potential‐like depolarizations in dissociated cholinergic nucleus basalis neurons of the guinea‐pig

Williams

Serafin²,

Mühlethaler³

et al. 1998

Eur J of Neuroscience

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The aim of the present study was to investigate whether the voltage-dependent inhibition of calcium currents by serotonin 5-HT1A agonists can be alleviated (facilitated) by action potential-like depolarizations. In dissociated cholinergic basal forebrain neurons using whole-cell recordings, it is shown that a selective serotonin 5-HT1A agonist (8-OH-DPAT) predominantly blocks N-type HVA calcium current, although a minor reduction of P-type current was also observed. The inhibition may principally occur through Gi-Go subtypes of G-proteins because it was prevented by N-ethylmaleimide, a substance known to block specifically pertussis-sensitive G-proteins. The inhibitory effect of 8-OH-DPAT on calcium currents is voltage-dependent because it was alleviated by long-lasting depolarizing prepulses. Interestingly, the inhibition could also be reversed by prepulses made-up of action potential-like depolarizations that were given at a frequency of 200 Hz. This observation may have important implications during periods of high-frequency rhythmic bursts, a firing pattern that is prevalent in cholinergic basal forebrain neurons.

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“…Besides opioid receptors, many other GPCRs expressed in DRG neurons were found to lead to a similar kind of calcium channel modulation. For example GABAB [190], adenosine A1 [191], 5-HT [163,192,193,194,195], P2Y [196], cannabinoid [197], neuropeptide Y Y2 receptors [198,199,200], somatostatin SST4 [201] or α2 adrenoceptors [202].…”

Section: Ion Channels As Targets Of Gpcr Signaling In Peripheral Nmentioning

confidence: 99%

Nociceptor Signalling through ion Channel Regulation via GPCRs

Salzer

Ray

Schicker

et al. 2019

IJMS

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The prime task of nociceptors is the transformation of noxious stimuli into action potentials that are propagated along the neurites of nociceptive neurons from the periphery to the spinal cord. This function of nociceptors relies on the coordinated operation of a variety of ion channels. In this review, we summarize how members of nine different families of ion channels expressed in sensory neurons contribute to nociception. Furthermore, data on 35 different types of G protein coupled receptors are presented, activation of which controls the gating of the aforementioned ion channels. These receptors are not only targeted by more than 20 separate endogenous modulators, but can also be affected by pharmacotherapeutic agents. Thereby, this review provides information on how ion channel modulation via G protein coupled receptors in nociceptors can be exploited to provide improved analgesic therapy.

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Serotonin inhibits high-threshold Ca2+ channel currents in capsaicin-sensitive acutely isolated adult rat DRG neurons

Cited by 27 publications

References 0 publications

Serotonergic modulation of the responses to excitatory amino acids of rat dorsal horn neurons in vitro: implications for somatosensory transmission

Serotonergic modulation of the responses to excitatory amino acids of rat dorsal horn neurons in vitro: implications for somatosensory transmission

The serotonin inhibition of high‐voltage‐activated calcium currents is relieved by action potential‐like depolarizations in dissociated cholinergic nucleus basalis neurons of the guinea‐pig

Nociceptor Signalling through ion Channel Regulation via GPCRs

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