Serotonin Producing Neuroepithelial Bodies in Rabbit Respiratory Mucosa

Lauweryns, Joseph M.; Cokelaere, Marnix; Theunynck, Paul

doi:10.1126/science.180.4084.410

Cited by 133 publications

(34 citation statements)

References 16 publications

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“…A 5-hydroxytryptaminergic neural innervation allowing EFS-evoked release of 5-HT also appears unlikely, as selective 5-HT receptor antagonists alone had no effect on EFS-induced excitatory NANC bronchoconstriction with the stimulation parameters we have tested. However this does not rule out the in vivo presence of endogenous 5-HT either from neuroepithelial cells, as in the rabbit (Lauweryns et al, 1973) or released from platelets activated during tissue damage. This released 5-HT may then serve to offset neurogenic inflammation resulting from sensory nerve activation.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of excitatory non‐adrenergic non‐cholinergic bronchoconstriction in guinea‐pig airways in vitro by activation of an atypical 5‐HT receptor

Ward

Fox

Barnes

et al. 1994

British J Pharmacology

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1 The effect of 5-hydroxytryptamine (5-HT) was studied on excitatory neurally mediated nonadrenergic non-cholinergic (NANC) contractions evoked by electrical field stimulation (EFS) in guineapig isolated bronchi. 2 5-HT (0.1-100 jM) produced a concentration-dependent inhibition of the excitatory NANC response with 50.9 ± 5.0% (n = 5, P<0.01) inhibition at 100 pM. This inhibition was not significantly affected by the 5-HT2 antagonist, ketanserin (1 AM) when inhibitions (± ketanserin) at each concentration of 5-HT were compared by unpaired t tests; however, this concentration appeared to produce a leftward shift 6 5-HT (10 AM) did not significantly affect contractile responses to exogenously applied substance P (1 nM-O 1Mm).7 The effect of 5-HT was unchanged after incubation with the nitric oxide (NO) synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 100 Mm). However, pretreatment with charybdotoxin (ChTX, 0.1-30 nM), a blocker of the large conductance Ca2l-activated K'channel (K+c.), produced a concentration-dependent inhibition of the effect of 5-HT (10MM). 8 5-HT evokes a concentration-dependent inhibition of e-NANC bronchoconstriction in guinea-pig isolated bronchi but does not affect cumulative concentration-dependent contractile responses to substance P, suggesting that inhibition is via a prejunctional receptor. Effects of selective antagonists and agonists suggest that an atypical 5-HT receptor mediates this inhibition. The inhibitory effect of 5-HT does not involve the production of NO, but may involve the opening a ChTX-sensitive K+ca channel. These data suggest that an atypical 5-HT receptor inhibits the release of neuropeptides from sensory C fibres and may act as other inhibitory neuromodulators via the opening of a common K'channel.

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Section: Discussionmentioning

confidence: 99%

Inhibition of excitatory non‐adrenergic non‐cholinergic bronchoconstriction in guinea‐pig airways in vitro by activation of an atypical 5‐HT receptor

Ward

Fox

Barnes

et al. 1994

British J Pharmacology

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“…Eaton and Fedde (57) identified two distinct populations of amine-containing cells in the mouse lung without preincubation with amine precursors; one population emitted a yellow fluorescence and the other a yellow-green fluorescence, which was possibly due to dopamine or norepinephrine. However, yellow fluorescence is the most often reported emission in lung neuroendocrine cells (18,29). Hage (4) reported that some cells of the bronchial mucosa of human fetuses exhibited a green fluorescence.…”

Section: Cytochemistrymentioning

confidence: 99%

“…There are lung epithelial cells in some species that contain enough endogenous biogenic amine to permit detection without prior administration of the amine precursor. NEBs of fetal or neonatal rabbits, for instance, reveal yellow fluorescent cells indicative of serotonin (29) (Fig. 13).…”

Section: Cytochemistrymentioning

confidence: 99%

“…Except when it is necessary to deal with the solitary neuroendocrine cells or the NEB independently, we shall refer to them collectively as neuroendocrine cells; solitary neuroendocrine cells will be referred to as K cells (92). The current status of (39,(78)(79)(80)(81); APUD = amine precursor uptake and decarboxylation characteristics (82); FIF = formaldehyde-induced fluorescence (20,30,81,83,84); EM = ultrastructural examination (81,85,86); NSE = neuron-specific enolase (7,87); AChE = acetylcholinesterase (84); CA = catecholamines (20,83,84); PAS-Pb-He = PAS-lead-hematoxylin (22,88); SEM = scanning electron microscopy (24,56,75); 5-HT = serotonin (29,30); DEN = diethylnitrosamine (50,(52)(53)(54).…”

Section: Nomenclaturementioning

confidence: 99%

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Neuroendocrinelike (small granule) epithelial cells of the lung.

DiAugustine¹,

Sonstegard²

1984

Environ Health Perspect

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The presence of neuroendocrinelike epithelial cells in the lung of numerous species has been demonstrated by light and electron microscopy. Histochemical methods used to identify these cells have included staining with silver, amine-type fluorescence (APUD cell), periodic acid Schiff (PAS)-lead hematoxylin, and immunohistochemical localization of neuron-specific enolase. Cytoplasmic dense core vesicles (70-200 nm in diameter) have served as the major ultrastructural characteristic. Lung neuroendocrinelike cells have been shown to occur in fetal and adult mammals as solitary-type cells or as distinct organoids known as neuroepithelial bodies ( NEBs ). Although the frequency of both populations is considered low, solitary-type cells with dense-core granules can be found in as high as 5% of epithelial cells in the cricoid region of the guinea-pig larynx. The solitary cells can be found throughout the airways of mammals, whereas the NEBs are confined to the intrapulmonary airways. Unmyelinated fibers have been traced from the lamina propria and into the NEB, where they ramified between the component cells of the NEB. The function of lung neuroendocrinelike cells is not known, but morphological and cytochemical studies suggest that the NEBs are intrapulmonary chemoreceptors that can respond to changes in airway gas composition. Hypoxia or hypercapnia has been shown to decrease the amine cytofluorescence in these organoids and apparently to increase the exocytosis of dense core vesicles from the basal region of the cell. Immunohistochemical studies have suggested that some lung epithelial cells may contain a known neuropeptide(s), but further investigation is needed to confirm the presence of such compounds in lung neuroendocrinelike cells and their physiochemical properties. Apparent hyperplasia of lung neuroendocrinelike cells can occur readily in hamsters treated with diethylnitrosamine. It has been postulated that human lung tumors with endocrinelike properties, namely, bronchial carcinoids and lung small cell carcinomas, may originate from lung neuroendocrinelike cells. However, a more plausible explanation, based on cytokinetic studies of epithelial neuroendocrinelike cells in the lung and other organs, is that these cells originate from a nonneuroendocrine population. Interaction of such a progenitor cell population with selected carcinogens may lead to stimulation of the rate of normal differentiation or, alternately, to selection of an abnormal route of differentiation that possesses a neuroendocrine phenotype. Images FIGURE 1. FIGURE 2. FIGURE 3. FIGURE 4. FIGURE 5. FIGURE 6. FIGURE 7. FIGURE 8. FIGURE 9. FIGURE 10. FIGURE 11. FIGURE 12. FIGURE 13. FIGURE 14. FIGURE 15.

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“…So far, these neuroendocrine epithelial (NEE) cells have been characterized primarily by morphological aspects and by their content of various peptides and amines (e.g Lauweryns et al, 1972;Cutz et al, 1975;1981;Track & Cutz, 1982;Polak & Bloom, 1982;Scheuermann, 1987;Adriaensen & Scheuermann, 1993). 5-Hydroxytryptamine (5-HT) is an important amine in the NEE cells (Dey et al, 1981;Lauweryns et al, 1973; where it appears to be stored in dense core vesicles (i.e. secretory granules of the NEE cells) (Owman et al, 1973;Cutz et al, 1975;Scheuermann, 1987;Adriaensen & Scheuermann, 1993).…”

Section: Introductionmentioning

confidence: 99%

Adrenoceptor‐ and cholinoceptor‐mediated mechanisms in the regulation of 5‐hydroxytryptamine release from isolated tracheae of newborn rabbits

Freitag

Wessler

Racké

1996

British J Pharmacology

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1 Isolated tracheae of newborn rabbits were incubated in vitro and the outflow of 5-hydroxytryptamine (5-HT) was determined by h.p.l.c. with electrochemical detection. Evidence has previously been provided that this 5-HT outflow derives from neuroendocrine epithelial (NEE) cells of the airway mucosa. 4 5-HT outflow evoked by 10 IM phenylephrine was inhibited by 65% in the presence of 1 gM forskolin and abolished in the presence of 10 gM forskolin. 5 5-HT outflow evoked by 10 gM phenylephrine was inhibited by about 45 and 70% in the presence of 0.1 and 1 gM isoprenaline, respectively. The inhibitory effect of 1 gM isoprenaline was only marginally antagonized by 1 gM, but blocked by 10 gM propranolol.6 5-HT outflow was not affected by the muscarine receptor agonist oxotremorine (10 gM), but was enhanced by 175% by 100 gM nicotine. The effect of nicotine was blocked by 100 gM hexamethonium and prevented by 1 gM tetrodotoxin or 1 gM yohimbine. 7 In conclusion, 5-HT release from NEE cells of the rabbit trachea is stimulated via a-adrenoceptors most likely of the a2B-subtype localized directly at the NEE cells. Activation of ,B-adrenoceptors as well as direct activation of adenylyl cyclase by forskolin exert inhibitory effects on 5-HT release. Activation of nicotinic, but not of muscarinic receptors, also evokes the release of 5-HT. However, the effect of nicotine appears to be mediated indirectly via the release of noradrenaline.

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Serotonin Producing Neuroepithelial Bodies in Rabbit Respiratory Mucosa

Cited by 133 publications

References 16 publications

Inhibition of excitatory non‐adrenergic non‐cholinergic bronchoconstriction in guinea‐pig airways in vitro by activation of an atypical 5‐HT receptor

Inhibition of excitatory non‐adrenergic non‐cholinergic bronchoconstriction in guinea‐pig airways in vitro by activation of an atypical 5‐HT receptor

Neuroendocrinelike (small granule) epithelial cells of the lung.

Adrenoceptor‐ and cholinoceptor‐mediated mechanisms in the regulation of 5‐hydroxytryptamine release from isolated tracheae of newborn rabbits

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