Serotoninergic activation of the basolateral amygdala and modulation of tonic immobility in guinea pig

Leite–Panissi, Christie Ramos Andrade; Ferrarese, Aline Aparecida; Terzian, Ana Luisa B.; Menescal-de-Oliveira, Leda

doi:10.1016/j.brainresbull.2006.02.007

Cited by 36 publications

(11 citation statements)

References 57 publications

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“…Our in vitro neurophysiology investigations suggest that 5-HT2 modulation of BLA principal neuron activity may be an important factor for these behavior outcomes. Our results also highlight the growing list of behavioral roles for BLA 5-HT2 receptors including the control of impulsive actions (Hadamitzky and Koch, 2009), innate anxiety-like behaviors (Leite-Panissi et al, 2006), and conditioned fear-like behaviors (Macedo et al, 2007). Future studies might employ 5-HT2A/C-focused approaches to determine the relative contributions of these specific behaviors to BLA-dependent reward-seeking.…”

Section: Discussionsupporting

confidence: 57%

Lateral/basolateral amygdala serotonin type-2 receptors modulate operant self-administration of a sweetened ethanol solution via inhibition of principal neuron activity

McCool

Christian

Fetzer

et al. 2014

Front. Integr. Neurosci.

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The lateral/basolateral amygdala (BLA) forms an integral part of the neural circuitry controlling innate anxiety and learned fear. More recently, BLA dependent modulation of self-administration behaviors suggests a much broader role in the regulation of reward evaluation. To test this, we employed a self-administration paradigm that procedurally segregates “seeking” (exemplified as lever-press behaviors) from consumption (drinking) directed at a sweetened ethanol solution. Microinjection of the nonselective serotonin type-2 receptor agonist, alpha-methyl-5-hydroxytryptamine (α-m5HT) into the BLA reduced lever pressing behaviors in a dose-dependent fashion. This was associated with a significant reduction in the number of response-bouts expressed during non-reinforced sessions without altering the size of a bout or the rate of responding. Conversely, intra-BLA α-m5HT only modestly effected consumption-related behaviors; the highest dose reduced the total time spent consuming a sweetened ethanol solution but did not inhibit the total number of licks, number of lick bouts, or amount of solution consumed during a session. In vitro neurophysiological characterization of BLA synaptic responses showed that α-m5HT significantly reduced extracellular field potentials. This was blocked by the 5-HT2A/C antagonist ketanserin suggesting that 5-HT2-like receptors mediate the behavioral effect of α-m5HT. During whole-cell patch current-clamp recordings, we subsequently found that α-m5HT increased action potential threshold and hyperpolarized the resting membrane potential of BLA pyramidal neurons. Together, our findings show that the activation of BLA 5-HT2A/C receptors inhibits behaviors related to reward-seeking by suppressing BLA principal neuron activity. These data are consistent with the hypothesis that the BLA modulates reward-related behaviors and provides specific insight into BLA contributions during operant self-administration of a sweetened ethanol solution.

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Section: Discussionsupporting

confidence: 57%

Lateral/basolateral amygdala serotonin type-2 receptors modulate operant self-administration of a sweetened ethanol solution via inhibition of principal neuron activity

McCool

Christian

Fetzer

et al. 2014

Front. Integr. Neurosci.

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“…While 5-HT3 activation in the amygdala appears to exert mostly anxiogenic effects (Mascagni and McDonald 2007), evidence for both anxiogenic and anxiolytic effects of modulating 5-HT1A and 5-HT2 receptor activation has been given (Menard and Treit 1999). The majority of recent findings points to primarily anxiolytic actions of 5-HT1A and anxiogenic actions of 5-HT2C (Christianson et al 2010; Leite-Panissi et al 2006; Li et al 2012; Morrison and Cooper 2012; Vicente and Zangrossi 2011). …”

Section: Discussionmentioning

confidence: 99%

Serotonergic innervation and serotonin receptor expression of NPY-producing neurons in the rat lateral and basolateral amygdaloid nuclei

et al. 2012

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Pharmacobehavioral studies in experimental animals, and imaging studies in humans, indicate that serotonergic transmission in the amygdala plays a key role in emotional processing, especially for anxiety-related stimuli. The lateral and basolateral amygdaloid nuclei receive a dense serotonergic innervation in all species studied to date. We investigated interrelations between serotonergic afferents and neuropeptide Y (NPY)-producing neurons, which are a subpopulation of inhibitory interneurons in the rat lateral and basolateral nuclei with particularly strong anxiolytic properties. Dual light microscopic immunolabeling showed numerous appositions of serotonergic afferents on NPY-immunoreactive somata. Using electron microscopy, direct membrane appositions and synaptic contacts between serotonin-containing axon terminals and NPY-immunoreactive cellular profiles were unequivocally established. Double in situ hybridization documented that more than 50 %, and about 30–40 % of NPY mRNA-producing neurons, co-expressed inhibitory 5-HT1A and excitatory 5-HT2C mRNA receptor subtype mRNA, respectively, in both nuclei with no gender differences. Triple in situ hybridization showed that individual NPY mRNA-producing interneurons co-express both 5-HT1A and 5-HT2C mRNAs. Co-expression of NPY and 5-HT3 mRNA was not observed. The results demonstrate that serotonergic afferents provide substantial innervation of NPY-producing neurons in the rat lateral and basolateral amygdaloid nuclei. Studies of serotonin receptor subtype co-expression indicate a differential impact of the serotonergic innervation on this small, but important, population of anxiolytic interneurons, and provide the basis for future studies of the circuitry underlying serotonergic modulation of emotional stimulus processing in the amygdala.

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“…Tonic immobility (TI) is a well-established test to evaluate the fear response in a wide range of vertebrates and invertebrates [4,5]. Long duration of TI is generally considered as an indication for high levels of fearfulness associating tonic immobility with emotional components like fear or anxiety [6] and with a fear-related phenotype [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of handling on neurotransmitter profile in pig brain according to fear related behaviour

Arroyo

Carreras

Valent

et al. 2016

Physiology & Behavior

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Serotoninergic activation of the basolateral amygdala and modulation of tonic immobility in guinea pig

Cited by 36 publications

References 57 publications

Lateral/basolateral amygdala serotonin type-2 receptors modulate operant self-administration of a sweetened ethanol solution via inhibition of principal neuron activity

Lateral/basolateral amygdala serotonin type-2 receptors modulate operant self-administration of a sweetened ethanol solution via inhibition of principal neuron activity

Serotonergic innervation and serotonin receptor expression of NPY-producing neurons in the rat lateral and basolateral amygdaloid nuclei

Effect of handling on neurotransmitter profile in pig brain according to fear related behaviour

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