Serotoninergic status in patients with hereditary vascular retinopathy syndrome

Abstract

Aim/background-In a new autosomal dominant syndrome (which the authors called hereditary vascular retinopathy (HVR)) cerebral ischaemia, Raynaud's phenomenon, and migraine are the most striking features. As serotonin (5-HT) is known to play a role in vasospastic processes, Raynaud's phenomenon, and migraine they wondered whether the serotoninergic status in patients with HVR is diVerent. Therefore, it was decided to investigate some serotoninergic variables in these patients. Methods-The study was conducted in 12 patients with HVR, 10 relatives, and 19 healthy controls. The levels of intraplatelet and plasma 5-HT were measured, as well as the plasma levels of its precursor amino acid tryptophan and the ratio of tryptophan to the large neutral amino acids, which compete with the transport of tryptophan through the blood-brain barrier. Results-In both the patients with HVR and in nine relatives the concentrations of 5-HT in plasma and platelets were significantly lower than in controls. The plasma levels of tryptophan and the tryptophan ratio were also found to be lower in the patient group compared with the control group, but not in the relatives. Conclusion-The observed alterations in 5-HT and its precursor tryptophan strongly suggest the existence of a malfunctioning of the serotoninergic system in the HVR syndrome. (Br J Ophthalmol 1998;82:897-900)

Paroxetine decreases platelet serotonin storage and platelet function in human beings

“…The platelet‐rich plasma–supernatant was then transferred into another tube (FACS tube; Becton Dickinson & Co). Platelets were concentrated from 2 mL platelet‐rich plasma through an additional run at 2650 g for 10 minutes 11 . The pellet was resuspended in 600 μL phosphate‐buffered saline solution, aliquoted (3 × 200 μL) into Eppendorf tubes and immediately stored at −80°C.…”