Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction

Furuya, Yumiko; Yoshida, Yuki; Katayama, Takashi; Yamamoto, Seigo; Kawamura, Akiyoshi

doi:10.1128/jcm.31.6.1637-1640.1993

Cited by 143 publications

(67 citation statements)

References 9 publications

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“…Nested PCR assays were performed using a modification of the method of Furuya et al, as previously described [8,10,11].…”

Section: Methodsmentioning

confidence: 99%

A serosurvey of Orientia tsutsugamushi from patients with scrub typhus

Kim¹,

Lee²,

Bäck³

et al. 2010

Clinical Microbiology and Infection

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Many countries where scrub typhus is endemic use their own cutoff values for antibody titres to differentiate between cured cases and current infections. To establish an antibody titre cutoff value, one needs to investigate the seroprevalence in endemic areas, and the duration of the increase in titre after complete cure. We conducted a follow-up study of anti-Orientia tsutsugamushi antibody titres using indirect immunofluorescence assays (IFA) and passive haemagglutination assays (PHA) in patients with scrub typhus. After the onset of symptoms, IgM antibody titres increased gradually over 2-3 weeks, peaked at about 4 weeks, and started to decrease rapidly between 4 and 5 weeks. At 1-year follow-up, the median IgM value was 1:10. Out of 77 patients who were tested at that time, 36 (47%) had IgM titres > or =1:20, and none had titres exceeding 1:80. Over the first 2 weeks, IgG antibody titres increased sharply, peaked at about 4 weeks and decreased rather gradually thereafter, with a median titre of 1:128 maintained up to the 18th month. At 1-year follow-up, five out of 77 patients (6.5%) had titres > or =1:1,024 and 57% had titres > or =1:128. Based on these results, a cutoff value of > or =1:160 for IgM antibody should differentiate between previous and current infections in endemic areas such as Korea and Japan, where scrub typhus occurs mainly in the autumn.

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“…Nested PCR assays were performed using a modification of the method of Furuya et al, as previously described [8,10,11].…”

Section: Methodsmentioning

confidence: 99%

A serosurvey of Orientia tsutsugamushi from patients with scrub typhus

Kim¹,

Lee²,

Bäck³

et al. 2010

Clinical Microbiology and Infection

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“…The target gene of this diagnostic PCR is the 56-kDa antigen gene of O. tsutsugamushi (3). Although this gene is widely conserved in O. tsutsugamushi, there are a few differences in nucleotide sequence among O. tsutsugamushi strains (4). Thus, two kinds of PCR methods have been introduced: those capable of detecting all the main strains of O. tsutsugamushi (called universal PCR), and those used to determine serotypes (called serotypic PCR) (4).…”

mentioning

confidence: 99%

“…Although this gene is widely conserved in O. tsutsugamushi, there are a few differences in nucleotide sequence among O. tsutsugamushi strains (4). Thus, two kinds of PCR methods have been introduced: those capable of detecting all the main strains of O. tsutsugamushi (called universal PCR), and those used to determine serotypes (called serotypic PCR) (4). As a positive control, native genomic DNA of Orientia is usually subjected to PCR in clinical examinations.…”

mentioning

confidence: 99%

Development of a pUC19‐based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi

Hanaoka

Sakamoto

Takano

et al. 2009

Microbiology and Immunology

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“…DNA extraction and amplification of the 56 kDa gene from O. tsutsugamushi strains (TH1817 and TC586 strains) and patients' blood samples was performed as described by Furuya et al (4). Whole blood samples from patients with febrile illness submitted to the Rickettsial unit, Institute for Medical Research were used.…”

mentioning

confidence: 99%

Sequence Analysis of the Hypervariable Regions of the 56 kDa Immunodominant Protein Genes of Orientia tsutsugamushi Strains in Malaysia

Tay

Rohani

et al. 2005

Microbiology and Immunology

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Abstract:The DNA sequences encompassing two hypervariable regions, VD II and III of the 56 kDa immunodominant protein gene of 21 Malaysian strains of Orientia tsutsugamushi were determined. Two strains demonstrated a 100% DNA homology with the Gilliam prototype strain, and one with TH1817 strain and TA678 strain respectively. High percentages of DNA similarity (95-99%) were observed with Karp (4 strains), Gilliam (2 strains), TH1817 (4 strains), TC586 (3 strains) and TA763 (1 strain). The remaining strains demonstrated the highest DNA similarity with TA763 (1 strain, 89%), TA678 (1 strain, 86%) and TA686 (1 strain, 87%). Our study provides additional evidence on the existence and the genetic heterogeneity of TA strains of the Southeast Asia and their closely related strains in Malaysia.

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Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction

Cited by 143 publications

References 9 publications

A serosurvey of Orientia tsutsugamushi from patients with scrub typhus

A serosurvey of Orientia tsutsugamushi from patients with scrub typhus

Development of a pUC19‐based recombinant plasmid to serve as a positive control in PCR for Orientia tsutsugamushi

Sequence Analysis of the Hypervariable Regions of the 56 kDa Immunodominant Protein Genes of Orientia tsutsugamushi Strains in Malaysia

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