Serotypes and virulence genes of extraintestinal pathogenic Escherichia coli isolates from diseased pigs in China

Tan, Chen; Tang, Xibiao; Zhang, Xuan; Ding, Yi; Zhao, Zhanqin; Wu, Bin; Cai, Xuwang; Liu, Zheng-Fei; He, Qigai; Chen, Huanchun

doi:10.1016/j.tvjl.2011.06.038

Cited by 42 publications

(71 citation statements)

References 28 publications

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“…A study by Tan et al showed that fimH , traT and iutA were highly prevalent in porcine ExPEC isolates, which is in consistent with our results, but the prevalence of fyuA , cnf1 , kpsMTII , and iroN differ between our study and theirs; the focG gene was not detected in any isolates in this study, which is similar to Tan’s research [25]. Interestingly, the results of VR gene detection in ExPEC isolates from retail pork is quite different from that from pigs on farms [26].…”

Section: Discussionsupporting

confidence: 93%

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Zhu

Wang

et al. 2017

BMC Vet Res

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BackgroundSwine extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that leads to economic and welfare costs in the swine industry worldwide, and is occurring with increasing frequency in China. By far, various virulence factors have been recognized in ExPEC. Here, we investigated the virulence genotypes and clonal structure of collected strains to improve the knowledge of phylogenetic traits of porcine ExPECs in China.ResultsWe isolated 64 Chinese porcine ExPEC strains from 2013 to 14 in China. By multiplex PCR, the distribution of isolates belonging to phylogenetic groups B1, B2, A and D was 9.4%, 10.9%, 57.8% and 21.9%, respectively. Nineteen virulence-related genes were detected by PCR assay; ompA, fimH, vat, traT and iutA were highly prevalent. Virulence-related genes were remarkably more prevalent in group B2 than in groups A, B1 and D; notably, usp, cnf1, hlyD, papA and ibeA were only found in group B2 strains. Genotyping analysis was performed and four clusters of strains (named I to IV) were identified. Cluster IV contained all isolates from group B2 and Cluster IV isolates had the strongest pathogenicity in a mouse infection model. As phylogenetic group B2 and D ExPEC isolates are generally considered virulent, multilocus sequence typing (MLST) analysis was performed for these isolates to further investigate genetic relationships. Two novel sequence types, ST5170 and ST5171, were discovered. Among the nine clonal complexes identified among our group B2 and D isolates, CC12 and CC95 have been indicated to have high zoonotic pathogenicity. The distinction between group B2 and non-B2 isolates in virulence and genotype accorded with MLST analysis.ConclusionThis study reveals significant genetic diversity among ExPEC isolates and helps us to better understand their pathogenesis. Importantly, our data suggest group B2 (Cluster IV) strains have the highest risk of causing animal disease and illustrate the correlation between genotype and virulence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-0975-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Zhu

Wang

et al. 2017

BMC Vet Res

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“…In general, most virulent extraintestinal E. coli strains belong to group B2 or D (28), whereas commensal strains (29) and strains derived from veterinary species (12) belong mostly to group A or B1. Our analysis of panda isolates showed that only a small fraction of isolates from fecal sources belonged to groups B2 and D, while 40% of vaginal isolates fell into these two phylogroups, which was in agreement with a previous study showing that 40% of porcine ExPEC strains belonged to these two virulent groups (12).…”

Section: Discussionsupporting

confidence: 92%

“…E. coli has been associated with systemic sepsis (9). E. coli strains from various animal species have been investigated extensively (10)(11)(12)(13)(14). However, little is known about the distribution and characteristics of E. coli in giant pandas.…”

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confidence: 99%

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Wang

Yan

Xia

et al. 2013

Appl Environ Microbiol

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f Although Escherichia coli typically colonizes the intestinal tract and vagina of giant pandas, it has caused enteric and systemic disease in giant pandas and greatly impacts the health and survival of this endangered species. In order to understand the distribution and characteristics of E. coli from giant pandas, 67 fecal and 30 vaginal E. coli isolates from 21 giant pandas were characterized for O serogroups, phylogenetic groups, antimicrobial susceptibilities, and pulsed-field gel electrophoresis (PFGE) profiles. In addition, these isolates were tested for the presence of extraintestinal pathogenic E. coli (ExPEC) and diarrheagenic E. coli (DEC) by multiplex PCR detection of specific virulence genes. The most prevalent serogroups for all E. coli isolates were O88, O18, O167, O4, and O158. ExPEC isolates were detected mostly in vaginal samples, and DEC isolates were detected only in fecal samples. Phylogenetic group B1 predominated in fecal isolates, while groups B2 and D were frequently detected in vaginal isolates. Resistance to trimethoprim-sulfamethoxazole was most frequently observed, followed by resistance to nalidixic acid and tetracycline. All except five isolates were typeable by using XbaI and were categorized into 74 PFGE patterns. Our findings indicate that panda E. coli isolates exhibited antimicrobial resistance, and potentially pathogenic E. coli isolates were present in giant pandas. In addition, these E. coli isolates were genetically diverse. This study may provide helpful information for developing strategies in the future to control E. coli infections of giant pandas.

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“…The frequency of isolation of ExPECs from diseased pigs increased from 3.1% in 2004 to 14.6% in 2007. Moreover, strains possessing similar virulence profiles and serogroups to human ExPECs have been detected from our collection of porcine ExPECs, suggesting that these pathogens may have strong zoonotic potential [6]. The virulence and zoonotic potential of porcine ExPECs may be linked to a specific clonal lineage within phylogenetic groups.…”

Section: Introductionmentioning

confidence: 99%

Clonal analysis and virulent traits of pathogenic extraintestinal Escherichia coliisolates from swine in China

et al. 2012

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BackgroundExtraintestinal pathogenic Escherichia coli (ExPEC) can cause a variety of infections outside the gastrointestinal tract in humans and animals. Infections due to swine ExPECs have been occurring with increasing frequency in China. These ExPECs may now be considered a new food-borne pathogen that causes cross-infections between humans and pigs. Knowledge of the clonal structure and virulence genes is needed as a framework to improve the understanding of phylogenetic traits of porcine ExPECs.ResultsMultilocus sequence typing (MLST) data showed that the isolates investigated in this study could be placed into four main clonal complexes, designated as CC10, CC1687, CC88 and CC58. Strains within CC10 were classified as phylogroup A, and these accounted for most of our porcine ExPEC isolates. Isolates in the CC1687 clonal complex, formed by new sequence types (STs), was classified as phylogroup D, with CC88 isolates considered as B2 and CC58 isolates as B1. Porcine ExPECs in these four clonal complexes demonstrated significantly different virulence gene patterns. A few porcine ExPECs were indentified in phylogroup B2, the phylogroup in which human ExPECs mainly exist. However some STs in the four clonal groups of porcine ExPECs were reported to cause extraintestinal infections in human, based on data in the MLST database.ConclusionPorcine ExPECs have different virulence gene patterns for different clonal complexes. However, these strains are mostly fell in phylogenentic phylogroup A, B1 and D, which is different from human ExPECs that concentrate in phylogroup B2. Our findings provide a better understanding relating to the clonal structure of ExPECs in diseased pigs and indicate a need to re-evaluate their contribution to human ExPEC diseases.

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Serotypes and virulence genes of extraintestinal pathogenic Escherichia coli isolates from diseased pigs in China

Cited by 42 publications

References 28 publications

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China

Serotypes, Virulence Factors, and Antimicrobial Susceptibilities of Vaginal and Fecal Isolates of Escherichia coli from Giant Pandas

Clonal analysis and virulent traits of pathogenic extraintestinal Escherichia coliisolates from swine in China

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