Serotypes of beta-hemolytic Treponema hyodysenteriae

Baum, David H.; Joens, Lynn A.

doi:10.1128/iai.25.3.792-796.1979

Cited by 88 publications

(43 citation statements)

References 19 publications

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“…LOSs extracted from T. hyodysenteriae are used as the primary antibody in ELISA serological confirmation for the presence of this organism in swine herds (10). For identification of the serotype of the causative agent in field cases of swine dysentery, LOS is extracted from the isolated organism and reacted with known antisera in Ouchterlony double-immunodiffusion tests (1).…”

Section: Discussionmentioning

confidence: 99%

“…Convalescent animals produce serotype-specific antibodies to the LPS-like structure of T. hyodysenteriae. This fact has been exploited in Ouchterlony double-immunodiffusion identification of strains differing in serospecificities (1). Of clinical importance, this has led to the development of accurate, serologically based enzyme-linked immunosorbent assay screening of swine herds affected by swine dysentery (10).…”

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confidence: 99%

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Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens

Halter

Joens

1988

Infect Immun

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Lipooligosaccharides from Treponema hyodysenteriae serotypes 1 through 7, attenuated T. hyodysenteriae serotypes 1 and 2, and five strains of T. innocens were extracted with hot phenol water. The extracts were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and analyzed by lipopolysaccharide selective silver staining and Western blot (immunoblot) immunodetection. Silver staining revealed the presence of two bands that ranged between 18,000 and 24,000 daltons and that were serotype specific for T. hyodysenteriae. Attenuation of pathogenic strains resulted in the loss of the higher-molecular-weight band. Four of five T. innocens strains also lacked this particular band. T. innocens 421 had six bands between 17,000 and 26,900 daltons. Western blots with hyperimmune rabbit sera and convalescent-phase swine sera revealed antigenic variation among serotypes of T. hyodysenteriae and attenuated serotypes of T. hyodysenteriae. Convalescent-phase swine sera failed to recognize lipopolysaccharides from T. innocens. Differences in results obtained by lipopolysaccharide selective silver staining versus immunoblotting of the lipopolysaccharide preparations probably indicate that these two methods identify separate characteristics of the same molecule.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens

Halter

Joens

1988

Infect Immun

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“…This condition has been identified as being the most economically significant endemic disease of pigs in Australia (Cutler and Gardner 1988). S hyodysenteriae isolates originally were divided into four serotypes on the basis of extracted lipopolysaccharide (LPS) (Baum and Joens 1979), and immunity against infection in colonic loops of recovered pigs was shown to be serotype-specific (Joens et a1 1983). Subsequently, bacterin vaccines were found to confer the best protection if infection was with a strain of the homologous serotype (Parizek et al 1985).…”

Section: )mentioning

confidence: 99%

Use of goose red blood cells for detection of infection with psittacine beak and feather disease virus by haemagglutination and haemagglutination inhibition

Sexton

Penhale

1994

Aust Veterinary J

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“…using lipopolysaccharide (LPS) extracted from T hyodysenteriae as the coating antigen for the microtitre plates*. Extraction and assay of LPS was as described by Baum and Joens (1979). Each serum was tested 3 times using the LPS extracted from isolates WA15, WA1 and WA6, respectively.…”

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confidence: 99%

A serological survey to determine the prevalence of infection with Treponema hyodysenteriae in Western Australia

1992

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A serological survey to detect antibody titres against Treponema hyodysenteriae was conducted on pigs from 106 herds in Western Australia. Titres indicating a positive result in the tests were determined by examining 400 sera from 4 herds known to be free of swine dysentery, and sera from immunised or experimentally infected pigs. Samples of serum from 40 bacon-weight pigs from each of the 106 herds were then collected at 2 abattoirs. Each serum was tested in enzyme-linked immunosorbent assays (ELISA) against the lipopolysaccharide of T hyodysenteriae of serogroups A, B and E, respectively. To assist in evaluating the test, 19 herds were resampled and retested, and faecal samples from 17 herds were cultured for T hyodysenteriae. Thirty-five of the 106 herds (33%) had serological evidence of infection when only one batch of sera from each herd was tested. The ELISA to detect T hyodysenteriae infection in herds using 40 sera was estimated as having a sensitivity of 77.3% and a specificity of 81.8% based on the owners' opinion of their herds disease status. Prevalence of infection within herds ranged from 2.5% to 47.5%, with a mean of 18%.

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Serotypes of beta-hemolytic Treponema hyodysenteriae

Cited by 88 publications

References 19 publications

Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens

Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens

Use of goose red blood cells for detection of infection with psittacine beak and feather disease virus by haemagglutination and haemagglutination inhibition

A serological survey to determine the prevalence of infection with Treponema hyodysenteriae in Western Australia

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