Serotypes of Dengue Viruses Circulating in Jazan Region, Saudi Arabia

Alsheikh, Adel A.; Daffalla, Ommer; Noureldin, Elsiddig; Mohammed, W. S.; Shrwani, Khalid; Hobani, Yahya Hasan; Abkar, Albokhary; Alsheikh, F. A.; Assiri, Abdullah

doi:10.21608/jesp.2017.77745

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The vaccination is not possible in developing countries against dengue disease due to high production cost plus the prevalence of more than one dengue virus strains i.e. DEN-I, DEN-II, DEN-III, and DEN-IV (Alsheikh et al ., 2017; Koo et al ., 2013). The vaccine for single serotype cannot diminish the risk of being infected with the other serotypes.…”

Section: Introductionmentioning

confidence: 99%

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CRISPR Cas9 mediated knockout of sex determination pathway genes in Aedes aegypti

Zulhussnain

Zahoor

Ranian

et al. 2022

Bull. Entomol. Res.

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The vector role of Aedes aegypti for viral diseases including dengue and dengue hemorrhagic fever makes it imperative for its proper control. Despite various adopted control strategies, genetic control measures have been recently focused against this vector. CRISPR Cas9 system is a recent and most efficient gene editing tool to target the sex determination pathway genes in Ae. aegypti. In the present study, CRISPR Cas9 system was used to knockout Ae. aegypti doublesex (Aaedsx) and Ae. aegypti sexlethal (AaeSxl) genes in Ae. aegypti embryos. The injection mixes with Cas9 protein (333 ng ul−1) and gRNAs (each at 100 ng ul−1) were injected into eggs. Injected eggs were allowed to hatch at 26 ± 1°C, 60 ± 10% RH. The survival and mortality rate was recorded in knockout Aaedsx and AaeSxl. The results revealed that knockout produced low survival and high mortality. A significant percentage of eggs (38.33%) did not hatch as compared to control groups (P value 0.00). Highest larval mortality (11.66%) was found in the knockout of Aaedsx female isoform, whereas, the emergence of only male adults also showed that the knockout of Aaedsx (female isoform) does not produce male lethality. The survival (3.33%) of knockout for AaeSxl eggs to the normal adults suggested further study to investigate AaeSxl as an efficient upstream of Aaedsx to target for sex transformation in Ae. aegypti mosquitoes.

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Section: Introductionmentioning

confidence: 99%

“…The vaccine for single serotype cannot diminish the risk of being infected with the other serotypes. High scale production of vaccines against all the serotypes at commercial level is a big challenge for economically poor countries (Alsheikh et al ., 2017). Therefore, it can only be eliminated through controlling its vector, Ae.…”

Section: Introductionmentioning

confidence: 99%

CRISPR Cas9 mediated knockout of sex determination pathway genes in Aedes aegypti

Zulhussnain

Zahoor

Ranian

et al. 2022

Bull. Entomol. Res.

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Screening of the normal bacterial flora in the gut of Aedes aegypti Mosquito in Saudi Arabia

Alghamdi

Farrag

Abdel‐Gaber

et al. 2023

Journal of King Saud University - Science

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Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

Alshabi

Marwan

Fatima

et al. 2022

Saudi Journal of Biological Sciences

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Dengue is an acute systemic viral disease that has been developed globally in both chronic and epidemic transmission periods. Dengue virus (DENV) is a member of the Flavivirus genus of the Flaviviridae family, which endangers public health. Limited studies have been performed in the Saudi Arabia and there are no epidemiological as well as molecular screening of DENV in the Southwestern region and this current study was conducted on the epidemiology of dengue in the Southwestern region of Saudi Arabia. Simultaneously, we have screened the 100 patients for DENV using the real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay. The current study results confirmed that 6637 people from various hospitals and locations in Jazan, Southwestern regions, were enrolled in this study from 2012 to 2020. The overall mean age was 30.02 ± 18.01 years, with 62.8% of males and 37.2% of females enrolled. This study included nearly three-fourths of the Saudi participants and one-fourth of the expatriates, and 56.6% of the positive cases were enrolled. In 2019, the most instances were enrolled, with 44% of positive cases. When screened using the RT-PCR assay, 93% of the positive patients were recruited, according to the quality control analysis. In conclusion, the current study results confirmed the prevalence of DENV was increased drastically since 2012 to 2020. High number of cases were registered prior to the Pandemic. The screening for DENV was performed with RT-PCR assay and NSI antigen should also be implemented to cross-check the results which was previously performed with RT-PCR analysis.

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Serotypes of Dengue Viruses Circulating in Jazan Region, Saudi Arabia

Cited by 4 publications

References 39 publications

CRISPR Cas9 mediated knockout of sex determination pathway genes in Aedes aegypti

CRISPR Cas9 mediated knockout of sex determination pathway genes in Aedes aegypti

Screening of the normal bacterial flora in the gut of Aedes aegypti Mosquito in Saudi Arabia

Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

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