Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in São Paulo, Brazil

Aidar-Ugrinovich, L.; Blanco, Miguel; Blanco, Jorge; Leomil, Luciana; Dahbi, Ghizlane; Mora, Azucena; Onuma, Daniela Lopes; Silveira, Wanderley Dias da; Castro, Antonio Fernando Pestana de

doi:10.1016/j.ijfoodmicro.2006.10.046

Cited by 104 publications

(112 citation statements)

References 57 publications

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“…Furthermore, to the best of our knowledge, this is the first study reporting the presence of saa in serotypes O20:H19, O86:H21, ONT:H4, and ONT:H18. All of the saa-positive strains were eaeA negative, supporting the hypothesis that these virulence factors are mutually exclusive (1,37,43,58,71,78,79). The eaeA gene is located in the LEE region of the chromosome, while the saa gene is found on pO113; thus there is no known reason as to why both could not be present in the same STEC strain.…”

Section: Discussionmentioning

confidence: 57%

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Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms

Monaghan

Byrne

Fanning

et al. 2011

Appl Environ Microbiol

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Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains are clinically significant food-borne pathogens. However, there is a dearth of information on serotype prevalence and virulence gene distribution, data essential for the development of public health protection monitoring and control activities for the meat and dairy industries. Thus, the objective of this study was to examine the prevalence of non-O157 STEC on beef and dairy farms and to characterize the isolates in terms of serotype and virulence markers. Bovine fecal samples (n ‫؍‬ 1,200) and farm soil samples (n ‫؍‬ 600) were collected from 20 farms throughout Ireland over a 12-month period. Shiga toxin-positive samples were cultured and colonies examined for the presence of stx 1 and/or stx 2 genes by PCR. Positive isolates were serotyped and examined for a range of virulence factors, including eaeA, hlyA, tir, espA, espB, katP, espP, etpD, saa, sab, toxB, iha, lpfA O157/OI-141 , lpfA O113 , and lpfA O157/OI-154 . Shiga toxin and intimin genes were further examined for known variants. Significant numbers of fecal (40%) and soil (27%) samples were stx positive, with a surge observed in late summer-early autumn. One hundred seven STEC isolates were recovered, representing 17 serotypes. O26:H11 and O145:H28 were the most clinically significant, with O113:H4 being the most frequently isolated. However, O2:H27, O13/O15:H2, and ONT:H27 also carried stx 1 and/or stx 2 and eaeA and may be emerging pathogens.

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Section: Discussionmentioning

confidence: 57%

“…Shiga toxin-producing Escherichia coli (STEC), also referred to as verocytotoxogenic Escherichia coli (VTEC), has emerged as a group of highly pathogenic Escherichia coli strains characterized by the production of one or more Shiga toxins (stx 1 , stx 2 , or their variants). STEC is common in ruminants and related foods (4,5,15,30,47).…”

mentioning

confidence: 99%

Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms

Monaghan

Byrne

Fanning

et al. 2011

Appl Environ Microbiol

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“…Paton & Paton (1998) first described the SAA in non--O157 STEC serotypes. Subsequently, several authors reported the presence of the saa gene in bovine and ovine STEC isolates, correlating the intestinal colonization in the host with the presence of this gene (Zweifel et al 2005, Aidar-Ugrinovich et al 2007). This study was the first to determine the presence of the saa gene in ovine STEC in the state of Goiás, reporting a prevalence of 47.7% (43/90).…”

Section: Discussionmentioning

confidence: 99%

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

et al. 2015

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“…The N-terminal region is conserved among the different intimin subtypes, while the C-terminal regions are highly variable. The 29 intimin subtypes are identified according to their C-terminal amino acid sequences [40][41][42][43][44][45][46]. Intimin-β is the most common subtype expressed in EPEC isolates [44][45][46]56].…”

Section: Immunogenicity and Efficacy Of A Roughmentioning

confidence: 99%

Vaccine Against Entheropathogenic E. colii: A Systematic Review

Vasconcellos¹,

Silva²,

Nascimento³

et al. 2017

IJVR

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Enteric diseases are a major cause of childhood death in the developing world, ranking as the second cause of death in children. Enteropathogenic Escherichia coli(EPEC) are important diarrheal pathogens of children under 5 years of age. Due to high mortality, several international organizations such as the WHO and UNICEF have dedicated preventive and control programs for diarrheal diseases. Among the suggested initiatives aiming to prevent infectious diarrhea include vaccine development and improvement in sanitation and water and food supplies.Upon contact with host cells, EPEC delivers an array of virulence protein factors, which integrated actions interferes with the normal adjusting the targets molecular cell functions leading to diarrhea. The locus of entherocyte effacement (LEE)pathogenicity island contains genes encoding synthesis of the EPEC virulence factors membrane adhesin intimin, T3SS (Esc and Sep proteins), chaperones (Ces proteins), translocators (EspA, EspB, and EspD), effector proteins (EspF, EspG, EspH, Map and EspZ), the translocated intimin receptor (Tir), and the regulatory protein Ler (LEEencoded regulator).Bundle-forming pilus (BfpA) is another virulence factor that mediates the initial contact between EPEC and the host cell. BfpA is encoded by a gene localized on a 50-70 MDa plasmid and is designated as EPEC adherence factor (EAF). BfpA, intimin and translocated Tir initiate EPEC infection. This repertoire of virulence factors offers strategic epitopes as vaccine candidates.Employing the proposed technologies for modern vaccines, recombinant Mycobacterium smegmatis (Smeg) and Mycobacterium bovis BCG strains were constructed to express BfpA, intimin and EspA. Recombinant clones were selected based on kanamycin resistance. Recombinant proteins are immunogenic, and the resultant antibodies recognize and block EPEC adhesion onto HEp-2 target cells. In attention to regulatory agency recommendations, kanamycin should not be used to produce rBCG expressing the E. coli EPEC virulence factors BfpA, intimin and EspA. Accordingly, an expression system with a Pasteur auxotrophic BCG mutant for leucine (BCG Pasteur ΔleuD) and a replicative vector (pUP410)should be used. Obtained recombinants will be grown in large scale, and their immunogenic effectivity and human safety submitted to WHO indicated quality controls.

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Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in São Paulo, Brazil

Cited by 104 publications

References 57 publications

Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms

Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Vaccine Against Entheropathogenic E. colii: A Systematic Review

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