Serotyping and bacteriophage typing of human and bovine group-B streptococci

Haug, Rangdi Holth; Gudding, Roar; Bakken, Gudbrand

doi:10.1099/00222615-14-4-479

Cited by 13 publications

(10 citation statements)

References 7 publications

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“…GBS bacteriophages have been used in a variety of phage typing systems since they are highly specific for different strains of the species (Haug et al, 1981;Stringer, 1980). In this work, we found that bacteriophage B30 lysed only a minor subgroup of serotype III GBS strains, those that do not secrete hyaluronate lyase.…”

Section: Discussionmentioning

confidence: 55%

“…Cloning and sequencing the GBS bacteriophage B30 lysin gene GBS strains often contain prophages that can be induced by various treatments (Haug et al, 1981). We exposed serotype III GBS strain 3330 to mitomycin C and recovered bacteriophages that gave small (~0?5 mm) clear plaques on a lawn of serotype III GBS strain 3331.…”

Section: Resultsmentioning

confidence: 99%

“…Bacteriophages of GBS were first isolated from bovine strains of the bacteria in 1969 (Russell et al, 1969). Phages were subsequently reported to be ubiquitous in human GBS strains and they formed the basis for various phage-typing systems for GBS (Haug et al, 1981;Stringer, 1980). Double-stranded DNA bacteriophages usually utilize a two-component lytic system that includes both a holin and a lysin (Grundling et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30

et al. 2004

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A group B streptococcal (GBS) bacteriophage lysin gene was cloned and expressed in Escherichia coli. The purified recombinant enzyme, calculated to have a molecular mass of 49 677 Da, lysed GBS cells. The susceptibility of GBS cells to lysis by the enzyme depended upon the growth stage at which they were harvested, with early exponential phase cells most sensitive. Calcium ions enhanced the activity of the enzyme. The enzyme also lysed other b-haemolytic streptococci, including groups A, C, E and G streptococci, but not common oral streptococci, including Streptococcus mutans. The generation of both reducing activity and N-terminal alanine residues during lysis indicated that the lysin is a bifunctional enzyme, possessing both glycosidase and endopeptidase activities. This is consistent with the presence of two conserved sequence domains, an Acm (acetylmuramidase) domain associated with lysozyme activity, and a CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain associated with endopeptidase activity. Site-directed mutagenesis of conserved cysteine and histidine residues in the CHAP domain and conserved aspartate and glutamate residues in the Acm domain confirmed their importance for lysozyme and endopeptidase activity respectively.

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Section: Discussionmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30

et al. 2004

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“…In an outbreak situation, phage typing was found to be very useful (Sechter et al 2000). However, GBS bacteriophages have been little studied untill now and just a few publications are available (Russel et al 1969;Stringer 1980;Haug et al 1983).…”

Section: Nongenetic Approaches To Epidemiology Of Group B Streptococcmentioning

confidence: 99%

“…In the USA, 7, 4, 11 and 10 different restriction patterns were revealed among 10 strains of serotype Ia, 4 strains of serotype Ib, 21 strains of serotype II and 14 strains of serotype III, respectively (Fasola et al 1993). The high degree of heterogeneity of PFGE patterns can be explained by integration of mobile genetic elements such as insertion sequences (Rubens et al 1989;Granlund et al 1998;Spellerberg et al 2000;Tamura et al 2000), transposons (Horaud et al 1996;Franken et al 2001) or bacteriophages (Russel et al 1969;Stringer 1980;Haug et al 1983) and other recombination events.…”

Section: Pulsed-field Gel Electrophoresismentioning

confidence: 99%

Molecular epidemiology of group B streptococcal infections

2004

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Streptococcus agalactiae (GBS) is a causative agent of sepsis and meningitis in newborns and diseases in pregnant women and nonpregnant adults. Various approaches, including both nongenetic and genetic techniques, are currently used for the study of epidemiology of GBS infections. In the present paper the different methods of molecular epidemiology of GBS infections are reviewed, and several novel approaches are introduced. The advantages and disadvantages of molecular methods are discussed and compared with traditional serotyping technique. The possible use of the molecular approaches for identification of different genetic lineages in GBS as well as for identification and control of the epidemiologically actual clones is discussed.

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Adherence of group B streptococci isolated from man and cattle to human and bovine epithelial cells

et al. 1983

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Proof of adherence of group B streptococci (GBS) to human and bovine vaginal epithelial cells and to bovine cells of milk cisternae of the mammary gland was employed as a criterion determining the possibility of colonization of these organs with GBS, or as another method of testing the transfer of GBS between man and cattle. GBS of both human and animal origin adhered to human epithelial cells in a similar way. On the other hand, a significantly stronger adherence of bovine GBS to vaginal epithelial cells and cells of milk cisternae of cattle was found than of human GBS. Thus the direction of colonization - animal is more probable than the opposite way. Neither in animal nor in human strains a correlation between the equipment of strains with type antigens and intensity of adherence could be found.

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Serotyping and bacteriophage typing of human and bovine group-B streptococci

Cited by 13 publications

References 7 publications

The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30

The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30

Molecular epidemiology of group B streptococcal infections

Adherence of group B streptococci isolated from man and cattle to human and bovine epithelial cells

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