Serotyping and esterase typing for analysis of Listeria monocytogenes populations recovered from foodstuffs and from human patients with listeriosis in Belgium

Gilot, Philippe; Genicot, A; André, Pascal

doi:10.1128/jcm.34.4.1007-1010.1996

Cited by 59 publications

(21 citation statements)

References 22 publications

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“…This indicates that serogroup 4 isolates are more virulent than those of serogroup 1 ⁄ 2. This is in line with the observation that serotype 4b isolates cause infection more frequently in previously healthy individuals than do serogroup 1 ⁄ 2 isolates [17], and that serotype 4b is over-represented among isolates from humans, compared with food sources where serogroup 1 ⁄ 2 isolates predominate [12][13][14]. However, the increased virulence of serogroup 4 isolates observed in the present study might be related also to the genetic lineage and not just the serotype.…”

Section: Discussionsupporting

confidence: 91%

Invasive listeriosis in Denmark 1994–2003: a review of 299 cases with special emphasis on risk factors for mortality

Gerner‐Smidt

Ethelberg

Schiellerup

et al. 2005

Clinical Microbiology and Infection

100

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Listeriosis is a rare, but serious, foodborne infection which, in the invasive form, presents as bloodstream (BS) infection, an infection of the central nervous system (CNS), a maternofetal infection or a focal infection. The disease is notifiable in Denmark. This paper reviews the results of the Danish surveillance of invasive listeriosis from 1994 to 2003, excluding maternofetal cases. In total, 299 invasive cases of listeriosis were reported. Two-thirds of the cases were caused by isolates of serogroup 1/2, and one-third by serogroup 4. Most (70%) cases had conditions known to predispose to listeriosis. More patients with BS infection were predisposed because of concurrent underlying illness than were patients with CNS infection. Half of the patients were aged > 70 years, and 21% died of the disease. There was no change in the case fatality rate (CFR) during the 10-year period. The CFR was identical for men and women. BS and CNS infection caused the same incidence of mortality, but no mortality was observed in patients with focal infections at normally sterile body sites. In a multivariate analysis, isolates belonging to serogroup 4 were associated with a higher CFR than were isolates of serogroup 1/2. In patients aged < 70 years, underlying conditions predisposing to disease were related strongly to mortality, which was not the case in patients aged > 70 years. The underlying conditions associated most strongly with mortality in the younger age group were non-haematological malignancies.

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Section: Discussionsupporting

confidence: 91%

Invasive listeriosis in Denmark 1994–2003: a review of 299 cases with special emphasis on risk factors for mortality

Gerner‐Smidt

Ethelberg

Schiellerup

et al. 2005

Clinical Microbiology and Infection

100

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“…Altogether, these results suggest that SSI-1 may enhance listerial adaptation to food environments. It is noteworthy that strains that generally lack the islet (serotype 4 strains) are less frequently detected in foods than other strains (Gilot et al 1996;Baek et al 2000;Choi et al 2001;Gilbreth et al 2005;Thévenot et al 2005). Transcriptional analysis confirmed that the islet genes are internally regulated by Lmo0445, suggesting that this regulator may contribute to the capacity of Listeria to respond and adapt to varying environmental conditions encountered either in foods or within the host, e.g.…”

Section: Discussionmentioning

confidence: 91%

A five-gene stress survival islet (SSI-1) that contributes to the growth of Listeria monocytogenes in suboptimal conditions

Ryan

Begley

Hill

et al. 2010

Journal of Applied Microbiology

151

150

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Aims: The aim of this study was to examine the contribution of a five‐gene islet (lmo0444 – lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. Methods and Results: Bioinformatics and PCR analyses revealed that a five‐gene islet is present in c. half of all L. monocytogenes strains examined (66 in total). A deletion mutant that lacks the entire c. 8·7‐kb islet was created in L. monocytogenes strain LO28. This mutant was impaired in growth at low pH and at high salt concentrations and demonstrated a decreased ability to survive and grow in a model food system (frankfurters). Transcriptional analysis revealed that the islet is self‐regulated in that the product of lmo0445 regulates the expression of the other four genes. A role of the alternative stress sigma factor SigB in regulating the islet was also uncovered. Conclusions: The five‐gene islet (herein designated as SSI‐1; stress survival islet 1) contributes to the growth of L. monocytogenes under suboptimal conditions. Significance and Impact of the Study: SSI‐1 may contribute to the survival of certain strains of L. monocytogenes in food environments.

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“…The genes necessary for internalization by host cells, escaping from phagosome to cytosol, and motility between host cells are harboured on a single pathogenicity island (PA1) (Roberts and Wiedmann, 2003). All L. monocytogenes are thought to possess this pathogenicity island even though not all strains of L. monocytogenes are pathogenic (Gilot et al, 1996;Muraoka et al, 2003;Remer et al, 2005). It is important to emphasize that L. monocytogenes has no recognized host reservoir.…”

Section: Introductionmentioning

confidence: 99%

Interactions between the environmental pathogen Listeria monocytogenes and a free‐living protozoan (Acanthamoeba castellanii)

Zhou

Elmose

Call³

2007

Environmental Microbiology

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Listeria monocytogenes can cause severe disease in animal hosts, but it has no recognized animal host reservoir. We tested the hypothesis that L. monocytogenes retains virulence traits to survive predation by amoebae and that listeriolysin O plays a crucial role in this process. Co-culturing of L. monocytogenes and Acanthamoeba castellanii demonstrated that L. monocytogenes does not actively kill amoebae, but in the presence of amoebae, high bacterial population densities can be maintained over a period of at least 96 h. A gentamicin protection assay demonstrated that there is no significant difference in the ability to survive predation between serovars (4b versus 1/2a and 1/2c; P = 0.08) and between five species of Listeria (P = 0.14). Three of these species do not harbour the hly gene responsible for listeriolysin O production. A hly knockout strain had poorer survival compared with the parental strain (P = 0.04 at 24 h; P = 0.04 at 48 h; P = 0.02 at 72 h) and electron microscopy was consistent with a wild-type strain being able to escape the phagosome whereas the hly knockout strain did not appear to have this ability. Thus, while there is weak evidence that listeriolysin O can contribute to improved survival after ingestion by amoebae, listeriolysin O does not appear to provide a significant selective advantage under the conditions of this study.

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Serotyping and esterase typing for analysis of Listeria monocytogenes populations recovered from foodstuffs and from human patients with listeriosis in Belgium

Cited by 59 publications

References 22 publications

Invasive listeriosis in Denmark 1994–2003: a review of 299 cases with special emphasis on risk factors for mortality

Invasive listeriosis in Denmark 1994–2003: a review of 299 cases with special emphasis on risk factors for mortality

A five-gene stress survival islet (SSI-1) that contributes to the growth of Listeria monocytogenes in suboptimal conditions

Interactions between the environmental pathogen Listeria monocytogenes and a free‐living protozoan (Acanthamoeba castellanii)

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