Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Machado, Sca; Pereira, Vla; Aquino, M. H. C.; Santos, A. J; Rodrigues, Daniel Sobreira; Giombelli, Audecir; Nascimento, E. R.

doi:10.1590/1806-9061-2016-0371

Cited by 7 publications

(7 citation statements)

References 12 publications

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“…Recently, research workers isolated S . Newport from chicken meat [28,29]. Report of S. Newport in a poultry farm and processing environment is a serious issue, and rapid rise of MDR Salmonella serovar Newport isolates over the past decade as important causes of human Salmonellosis [30].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

et al. 2018

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Background and Aim:The extensive use of antimicrobials in poultry has led to an increase in bacterial multidrug resistance, and the emergence of multidrug-resistant nontyphoidal Salmonella is a global problem. This study was performed to detect antibiotic-resistant Salmonella serovars in poultry farming and processing environment.Materials and Methods:A total of 956 various samples, comprising 432 farm origin, 324 poultry processing stage wise and environmental, and 154 product processing stages and environmental samples, were collected from poultry farms and processing units located in and around Mumbai city. Of a total of 71 recovered isolates, 42 randomly selected Salmonella isolates were subjected for antibiotic susceptibility testing by disk diffusion method and serotyping. A total of 31 serotypically confirmed isolates were characterized for the presence of tetA, tetB, blaTEM, and CTX-M gene.Results:Higher resistance was recorded against Doxycycline (100%), followed by Oxytetracycline (97.62%), Neomycin (88.10%), Erythromycin (83.33%), Tetracycline (78.57%), and Ceftizoxime (35.71%). Resistance from 0.00 to 26.19 percent was found to antimicrobials, namely Norfloxacin (26.19%), Ampicillin (21.43%), Azithromycin (21.43%), Ciprofloxacin (19.05%), Colistin (4.76%), Streptomycin (16.67%), Cefotaxime (14.19%), Enrofloxacin (14.29%), Amoxyclav (14.29%), Gentamicin (7.14%), Chloramphenicol (4.76%), Amikacin (4.76%), and Ceftazidime (0.0%). Results demonstrate that the Salmonella Virchow dominated and all serotypes were found to carry Tetracycline resistance gene tetA, 5 isolates were found to be positive for blaTEM, whereas none of the isolates were carrying tetB and CTX-M gene.Conclusion:This study revealed that there is a significant rise of Tetracycline resistance with the presence of tetA gene in Salmonella spp. which indicates selective pressure for adopting resistance against tetracycline group of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

et al. 2018

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“…At the same time, its ability to form biofilms stands out; this hinders its control in the food industry [ 16 ]. In this condition, microorganisms have different responses to heat treatments, biocides, and antimicrobials, and are therefore a constant source of contamination [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

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The presence of virulence genes, phylogenetic relationships, biofilm formation index (BFI), and ultrastructure in S. Minnesota at different temperatures (4, 25, and 36 °C) were analyzed. In addition, the ability of biocidal agents (chlorhexidine1%, sodium hypochlorite 1%, and peracetic acid 0.8%) to inhibit biofilms formed by 20 strains isolated from broiler slaughter plants from two Brazilian companies in 2009, 2010, and 2014 was determined. The presence of specific genes was evaluated by PCR and phylogeny between strains by pulsed-field gel electrophoresis. The BFI was determined using tryptone soy broth with 5% of chicken juice, and its structure was observed by scanning electron microscopy. The presence of specific genes indicated that S. Minnesota has the potential to cause disease in humans, adapting to adverse conditions. Temperatures of 25 and 36 °C favored biofilm formation, although at 4 °C, there was still biomass that could contaminate the final product. Tolerance to all biocides was identified in 12/20 (60%), representing a real risk of adaptation mechanisms development, especially regarding to resistance to sodium hypochlorite. Phylogenetic analysis indicated cross-contamination and spread among companies, which was probably related to biofilms formation. Results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite, including the need for rigorous control, adopting low temperatures to prevent biofilms formation in the poultry industry.

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“…At the same time, among the hypotheses that may explain S. Minnesota persistence in the environment, its ability to form bio lms stands out, which hinders its control in the food industry [46]. In this condition microorganisms have different responses to heat treatments, biocides and antimicrobials, and are therefore a constant source of contamination [18,27,40].…”

Section: Introductionmentioning

confidence: 99%

Salmonella Minnesota Emergence in Poultry Production: Danger to Public Health?

Melo¹,

Cardoso²,

Peres³

et al. 2020

Preprint

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Purpose: The aim of this study was to analyze in S. Minnesota the presence of virulence genes, phylogenetic relationship, biofilm formation index (BFI) and its ultrastructure at temperatures of 4 °C, 25 °C and 36 °C and also the ability of biocidal agents to promote inhibition of the sessile structure formed by 20 strains isolated in the years 2009, 2010 and 2014 in broiler slaughter plants of two Brazilian companies.Methods: The presence of specific genes was evaluated by PCR and the phylogeny between the strains by PFGE, while BFI was performed by the classical method using TSB broth supplemented with 5% of chiken juice and its structure observed by SEM Chlorhexidine 1%, sodium hypochlorite 1% and peracetic acid 0.8% were tested for biofilm inhibition.Results: The presence of specific genes indicates that S. Minnesota has the potential to cause disease in humans and to adapt to adverse conditions. In general, temperatures of 25 °C and 36 °C favored more biofilm formation, although at 4 °C there is still biomass with potential contaminant from the final product. Tolerant strains (12/20–60%) to all biocides pose a real risk for the development of adaptation mechanisms, especially for resistance to sodium hypochlorite. Phylogenetic analysis indicated the existence of cross contamination problems and the spread among companies, probably related to the formation of biofilms.Conclusion: The results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite in addition to the need for rigorous monitoring and control measures based on the adoption of low temperatures in order to prevent the formation of biofilms in the poultry industry.

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Serotyping and Genotyping of Salmonella Strains Isolated From Broilers, Chicken Carcasses Before and After Chilling, and Frozen Chicken Breasts Produced in The States of Mato Grosso do Sul and Santa Catarina, Brazil

Cited by 7 publications

References 12 publications

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Salmonella Minnesota Emergence in Poultry Production: Danger to Public Health?

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