Serotyping of
            <i>Chlamydia</i>
            I. Isolates of Ovine Origin

Schachter, Julius; Banks, Joyce; Sugg, Nancy; Sung, Minnie; Storz, J.; Meyer, K. F.

doi:10.1128/iai.9.1.92-94.1974

Cited by 60 publications

(25 citation statements)

References 7 publications

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“…Storz and his coworkers (28,32,33) showed 8 biotypes in avian and mammalian C. psittaci by analysis of morphological differences of inclusion bodies and biological effects of diethylaminoethyl (DEAE)-dextran and cycloheximide in their growth medium, and further revealed 9 immunotypes in mammalian C. psittaci by applying microimmunofluorescent test (MIF). Eb and Orfila (4) also confirmed the 2 serotypes of Schachter et al (30,31). Recently Anderson (1) reported serotyping by use of typespecific monoclonal antibodies to avian and mammalian C. psittaci.…”

Section: Introductionmentioning

confidence: 76%

“…Many efforts were attempted to classify C. psittaci. Schachter et al (30,31) showed 2 serotypes in mammalian C. psittaci using plaque neutralization tests. Storz and his coworkers (28,32,33) showed 8 biotypes in avian and mammalian C. psittaci by analysis of morphological differences of inclusion bodies and biological effects of diethylaminoethyl (DEAE)-dextran and cycloheximide in their growth medium, and further revealed 9 immunotypes in mammalian C. psittaci by applying microimmunofluorescent test (MIF).…”

Section: Introductionmentioning

confidence: 99%

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Chlamydia pecorum the Fourth Species of Genus Chlamydia

福士¹,

平井²

1995

J.Jpn.Vet.Med.Assoc.

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Chlamydia pecorum the Fourth Species of Genus Chlamydia

福士¹,

平井²

1995

J.Jpn.Vet.Med.Assoc.

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“…Previous studies for C. psittaci immunological heterogeneity by the plaque reduction technique (33,34) and immunofluorescence (2,16,32,39) did not define the components on the outer membrane responsible for the differences observed. Furthermore, few attempts to compare the antigenic structure of this species with that of C. trachomatis have been made.…”

mentioning

confidence: 81%

Identification of genus-specific epitopes on the outer membrane complexes of Chlamydia trachomatis and Chlamydia psittaci immunotypes 1 and 2

et al. 1989

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Polyclonal and monoclonal antibodies were used to study the immunogenic and antigenic characteristics of chlamydiae. We focused on the most predominant proteins in the outer membrane complex, the major outer membrane protein (MOMP) and the doublet consisting of proteins of 57 and 62 kilodaltons (57-62 kDa doublet). Immunoblot analyses were performed with chlamydial elementary bodies by using (i) immune sera from sheep which had undergone a recent episode of abortion due to the ovine abortion (OA) strain of C. psittaci, (ii) rabbit hyperimmune anti-C. psittaci (OA) and -C. trachomatis sera, and (iii) monoclonal antibodies to the MOMP of C. trachomatis. The typical pattern of response with polyclonal antisera against heterologous elementary bodies was reactivity with the 57-62 kDa doublet and lipopolysaccharide with weak and sometimes no anti-MOMP activity. Three distinct genus-specific anti-C. trachomatis MOMP monoclonal antibodies showed different patterns of reactivity with the MOMPs of the two immunotypes of C. psittaci and C. trachomatis serovars. Our data confirm the predominance of a genus-specific 57-62 kDa doublet response despite the presence of genus-specific epitopes on the MOMP.Interaction of Chlamydia psittaci with mouse peritoneal macrophages.

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“…Fraser and Berman (4) clearly demonstrated that the strains associated with EAE were antigenically different from the polyarthritis and ovine pneumonitis isolates by using purification of elementary bodies with fluorocarbon and different procedures involving marked modification of antigens. A yolk sac infectivity neutralization test (11,20) and more recently a plaque reduction test (7,8) have also been used. Isolates associated with abortion and the M0907 strain could be separated from isolates associated with polyarthritis and conjunctivitis; there was no cross-reaction between these two sero-types or with several avian strains (7).…”

mentioning

confidence: 99%

“…A yolk sac infectivity neutralization test (11,20) and more recently a plaque reduction test (7,8) have also been used. Isolates associated with abortion and the M0907 strain could be separated from isolates associated with polyarthritis and conjunctivitis; there was no cross-reaction between these two sero-types or with several avian strains (7). These two serotypes were not host specific (8).…”

mentioning

confidence: 99%

Serotyping of Chlamydia psittaci by the Micro-Immunofluorescence Test: Isolates of Ovine Origin

Orfila

1982

Infect Immun

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The micro-immunofluorescence test was successfully applied to seven ovine isolates which could be separated into two distinct immunotypes. The pattern of reactivity was similar to that elucidated by the plaque reduction test and confirmed by growth characteristics and biotyping.The micro-immunofluorescence (IF) test has been used for immunoclassification of Chlamydia trachomatis strains (12,(14)(15)(16)(17)19). Recently, we used this method for serotyping six strains of Chlamydia psittaci recovered from birds, sheep, mice, and humans (2). This note reports the results obtained with this technique applied to seven isolates recovered from sheep and associated with abortion, polyarthritis, and inapparent infection.Five strains isolated from placentas or aborted lambs and associated with abortion were tested: Q18 and EAE Nice (Edlinger; Institut Pasteur, Paris); EAE Tours (Rodolakis; INRA, Nouzilly, France); A22 (J. D. Treharne; Institute of Ophthalmology, London); and B577 (J. Storz; Colorado State University, Fort Collins, Colo.). The M0907 strain, isolated from the feces of an apparently normal sheep (J. Storz), and the LW679 strain, isolated from ajoint and associated with polyarthritis (J. Storz), were also tested, as were the 6BC strain of C. psittaci (J. Schachter, University of California, San Francisco) and a human genital strain of C. trachomatis, LB1 (TRIC/D/GB/MRC-1/G) (Hanna; Proctor Institute, San Francisco).Isolates were propagated in chicken embryos; suspensions for slide antigens were titrated by the method of Reeve and Taverne (5) and stored at -70°C. The micro-IF technique and the immunization schedule have been described by Wang and Grayston (16). Some modifications were made: (i) all antigens for immunization were prepared in HeLa 229 or McCoy cell cultures; (ii) antisera were prepared in inbred mice (2) chosen for their resistance to the chlamydial strains (C3H mice for the isolates associated with abortion and the M0907 strain; C57BL

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Serotyping of Chlamydia I. Isolates of Ovine Origin

Cited by 60 publications

References 7 publications

Chlamydia pecorum the Fourth Species of Genus Chlamydia

Chlamydia pecorum the Fourth Species of Genus Chlamydia

Identification of genus-specific epitopes on the outer membrane complexes of Chlamydia trachomatis and Chlamydia psittaci immunotypes 1 and 2

Serotyping of Chlamydia psittaci by the Micro-Immunofluorescence Test: Isolates of Ovine Origin

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