Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Morisset, Stéphane; Peyron, François; Lobry, Jean R.; Garweg, Justus G.; Ferrandiz, Josette; Musset, Karine; Gómez-Marín, Jorge Enrique; de-la-Torre, Alejandra; Demar, Magalie; Carme, Bernard; Mercier, Corinne; Garin, Jean François; Cesbron-Delauw, Marie-France

doi:10.1016/j.micinf.2008.04.001

Cited by 67 publications

(50 citation statements)

References 29 publications

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“…Therefore, a larger sample size should be carried out in human patients to identify specific T. gondii strains using high resolution typing methods such as multiplex nested PCR-RFLP which can genotype some DNA samples extracted directly from infected tissues. Serotyping of T. gondii strains has proved to be a promising tool using serum samples to overcome the diagnostic challenge (Kong et al, 2003;Peyron et al, 2006;Morisset et al, 2008;Sousa et al, 2008;Sousa et al, 2009). However, due to the limitation of serotyping it is not possible to differentiate type II from non-type II strains particularly in South America where there is a high diversity of T. gondii strain types.…”

Section: Other Diagnostic Methodsmentioning

confidence: 99%

Toxoplasmosis in HIV/AIDS Patients - A Living Legacy

Nissapatorn¹

2011

Microbes, Viruses and Parasites in AIDS Process

101

124

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Section: Other Diagnostic Methodsmentioning

confidence: 99%

Toxoplasmosis in HIV/AIDS Patients - A Living Legacy

Nissapatorn¹

2011

Microbes, Viruses and Parasites in AIDS Process

101

124

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“…For example, type II strains are involved in Ͼ96% of consecutive cases of congenital toxoplasmosis in France (2), in 85% of immunocompromised patients who acquired Toxoplasma infection in Europe (4), and in up to 100% of animals from several European countries (from 73% to 100%) (10,13,18). Furthermore, serotyping analysis confirmed the predominance of type II serotypes not only in chronically infected persons but also in patients with ocular disease in Europe (26,28).…”

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confidence: 97%

Direct Genotyping of Toxoplasma gondii in Ocular Fluid Samples from 20 Patients with Ocular Toxoplasmosis: Predominance of Type II in France

et al. 2011

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We report the direct genotyping analysis of Toxoplasma gondii in ocular samples collected from 20 patients, as well as associated clinical and epidemiological data. This work was aimed at better understanding the impact of genotypes of Toxoplasma gondii strains on toxoplasmic retinochoroiditis. For this purpose, we studied the aqueous humor (AH) or vitreous humor (VH) of 20 patients presenting with ocular toxoplasmosis (OT) in 2 hospitals in France. Genetic characterization was obtained with microsatellite markers in a multiplex PCR assay. In contrast to the results of previous studies, we found no association between atypical Toxoplasma gondii genotypes and the occurrence of OT. Considering the local epidemiological data, our OT patients seemed to be infected more frequently by ordinary type II strains found in the environment. In conclusion, direct genotyping of Toxoplasma gondii strains from aqueous or vitreous humor showed a predominance of the type II genotype in ocular toxoplasmosis; this may be due to a high exposure rate of this genotype in humans.

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“…For more than a decade there was a debate over the efficacy of prenatal treatment; however recent publications that include a systematic review comparing cohorts in Europe, North America and South America 6 , as well other observational studies 8 , indicate there is a benefit in prenatal treatment to prevent serious conditions in the infant. On the other hand, findings indicate that in South America, the infection is more severe than in Europe or North America, not only because of the higher frequency of cases but because there are more severe clinical presentations and a higher mortality rate 6,7 , given the higher virulence of the strains found in this geographical area [8][9][10][11] .…”

Section: Introductionmentioning

confidence: 92%

“…In Colombia, more than half of pregnant women (50-60%) have anti-Toxoplasma antibodies, suggesting there is a high exposition and circulation of the parasite in the country 12,11 . It is expected that between 0.6 to 3% of women acquire the infection during pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Clinical practice guideline. Integral Care Guidelines for the prevention, early detection and treatment of pregnancy, childbirth and puerperium complications: Section on toxoplasmosis in pregnancy

Cortés

Gómez

Silva

et al. 2017

Infect

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Cómo citar este artículo: Cortes JA, et al. Clinical practice guideline. Integral Care Guidelines for the prevention, early detection and treatment of pregnancy, childbirth and puerperium complications: Section on toxoplasmosis in pregnancy. Infectio 2017. http://dx.doi.org/10.22354/in.v21i2.654 AbstractToxoplasma infection, an aspect included in the Integral Care Guidelines for prevention, early detection and treatment of complications during pregnancy developed and sponsored by the Department of Science, Technology and Innovation, Colciencias and the Ministry of Health and Social Protection; was selected to develop recommendations that may contribute to its prevention diagnostic and treatment. Guidelines were developed through systematic review of literature, two panels of evaluation, gradation of evidence: SIGN (Scottish Intercollegiate Guidelines Network), discussion panel with users and patients, external evaluation and socioeconomical evaluation of the impact of recommendations. The Toxoplasma gondii infection that occurs during pregnancy may cause serious complications for the fetus and important sequelae for the newborn. A clinical guideline based on the best available scientific evidence, with emphasis on Colombian references, was performed. Experts in parasitology, neonatology and infectious diseases (adult and pediatric) developed the recommendations. It is suggested that the recommendations included in this guideline be followed by healthcare professionals in pregnancy care programs nationwide, with the purpose of lowering the morbidity and mortality related to this disease. Specific recommendations for diagnosis during the first trimester of the pregnancy, recommendations for prevention in noninfected women, identification of fetal or newborn infection and recommendations for treatment in those given cases are included. Guia de atencion integral para la prevencion, deteccion temprana y tratamiento de las complicaciones del embarazo: Sección de Toxoplasmosis ResumenComo parte de la Guia de atencion integral para la prevencion, deteccion temprana y tratamiento de las complicaciones del embarazo, desarrollada y financiada por el Departamento de Ciencia, Tecnologia e Innovacion, Colciencias, y el Ministerio de Salud y Proteccion Social de Colombia, se seleccionó a la infeccion por Toxoplasma para el desarrollo de recomendaciones para su prevencion, diagnostic° y tratamiento. La infeccion por Toxoplasma gondii (T. gondii) durante el embarazo puede resultar en graves complicaciones para el feto y dejar importantes secuelas al recien nacido. Se realizo una guia basada en la mejor evidencia disponible en ARTÍCULO ORIGINAL Integral Care Guidelines for the prevention, early detection and treatment of pregnancy, childbirth and puerperium complications. 103la literatura cientifica, con especial pertinencia a la informacion colombiana. Un consenso de expertos en parasitologia, ginecologia, neonatologia e infectologia, tanto de adultos como pediatrica, desarrollo las recomendaciones. Se propone que las recomendac...

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Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Cited by 67 publications

References 29 publications

Toxoplasmosis in HIV/AIDS Patients - A Living Legacy

Toxoplasmosis in HIV/AIDS Patients - A Living Legacy

Direct Genotyping of Toxoplasma gondii in Ocular Fluid Samples from 20 Patients with Ocular Toxoplasmosis: Predominance of Type II in France

Clinical practice guideline. Integral Care Guidelines for the prevention, early detection and treatment of pregnancy, childbirth and puerperium complications: Section on toxoplasmosis in pregnancy

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