Serotyping of Vibrio anguillarum

Abstract

A serotyping scheme based on the detection of 0 antigens by slide agglutination in fish-pathogenic strains of Vibrio anguillarum is presented. Over a period of 5 years 270 Vibrio strains from feral and cultured fish, 189 strains from the environment, and 36 strains from invertebrates were collected. The strains were divided into 10 distinct serotypes (01 through 010). More than 90% of the fish-pathogenic strains, but only 40% of the environmental strains, were typable; 71% of the strains isolated from cultured rainbow trout were serotype 01, whereas 78% of the strains isolated from feral fish were serotype 02. No dominating environmental serotype was found. A serotyping system for V. anguillarum is proposed. A total of 90 strains received from culture collections and laboratories in different countries were typed according to the present system.

Role of the metalloprotease Vvp and the virulence plasmid pR99 of Vibrio vulnificus serovar E in surface colonization and fish virulence

“…Antisera against whole cells of CECT4999 were obtained as previously described (Sorensen and Larsen, 1986). All sera were titrated against the homologous strain by dot blotting as previously described (Cipriano et al, 1985) and were stored in aliquots and frozen at -80°C until used.…”

Structural characterization of the lipopolysaccharide O‐antigen and capsular polysaccharide of Vibrio ordalii serotype O : 2

“…Both polymers were shown to be composed of linear tetrasaccharide repeating units, having the structure :where Am ϭ acetamidino; Fo ϭ formyl; AN ϭ amide, and Sug ϭ 2-acetamido-2,6-dideoxy-D-xylohexos-4-ulose.Keywords : lipopolysaccharide ; capsular polysaccharide ; Vibrio ordalii; NMR.Vibrio anguillarum is one of the most common causes of ture of the LPS O-chain and CPS from V. anguillarum serotype O:2 [7]. The purpose of the present study was to isolate and vibriosis among feral and farmed fish and shellfish [1]. Once referred to as V. anguillarum biotypes 1 and 2, biotype 2 has characterise the cell-surface antigens from V. ordalii, in order to provide immunochemical rationale for the serological relation-been classified as a separate species V. ordalii [2, 3].…”

“…V. anguillarum serotypes O:1 and O :2 and V. ordalii strains were found ship between V. ordalii and V. anguillarum O:2 strains. to cause the majority of the infections in salmonid fish [1]. Based on the agglutination reaction with rabbit serum against V. anguillarum serotype O :2 strains, V. ordalii strains were classi-EXPERIMENTAL PROCEDURES fied as serotype O :2 pathogens [1].…”

“…Vibrio anguillarum is one of the most common causes of ture of the LPS O-chain and CPS from V. anguillarum serotype O:2 [7]. The purpose of the present study was to isolate and vibriosis among feral and farmed fish and shellfish [1]. Once referred to as V. anguillarum biotypes 1 and 2, biotype 2 has characterise the cell-surface antigens from V. ordalii, in order to provide immunochemical rationale for the serological relation-been classified as a separate species V. ordalii [2, 3].…”

“…It was shown by sugar and methylation analysis that the glucan, which NMR spectroscopy. 1 H-and 13 C-NMR spectra were obtained with a Bruker AMX 500 or AMX 600 spectrometer using co-extracted with the LPS from V. ordalii serotype O:2, had a branched glycogen-type structure, similar to that of the glucan standard Bruker software. The observed 1 H and 13 C chemical shifts are reported relative to the methyl group of internal ace-from V. anguillarum serotype O :2 [7].…”