Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs

Bird, Lina J.; Kuenen, J.G.; Osburn, M. R.; Tomioka, Naotaka; Ishii, Shun’ichi; Barr, Casey; Nealson, Kenneth H.; Suzuki, Shino

doi:10.1099/ijsem.0.004945

Cited by 25 publications

(9 citation statements)

References 43 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the relative abundances of Hydrogenophaga/Serpentinomonas and unclassified Comamonadaceae on mica schist were the highest in the methanol-amended microcosms. Hydrogenophaga and Serpentinomonas strains have been shown to grow on acetate and formate ( Bird et al, 2021 ). Correspondingly, one mica schist epilithic Serpentinomonas MAG had genes for acetate metabolism ( Nuppunen-Puputti et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater

et al. 2023

View full text Add to dashboard Cite

Microbial life in the deep subsurface occupies rock surfaces as attached communities and biofilms. Previously, epilithic Fennoscandian deep subsurface bacterial communities were shown to host genetic potential, especially for heterotrophy and sulfur cycling. Acetate, methane, and methanol link multiple biogeochemical pathways and thus represent an important carbon and energy source for microorganisms in the deep subsurface. In this study, we examined further how a short pulse of low-molecular-weight carbon compounds impacts the formation and structure of sessile microbial communities on mica schist surfaces over an incubation period of ∼3.5 years in microcosms containing deep subsurface groundwater from the depth of 500 m, from Outokumpu, Finland. The marker gene copy counts in the water and rock phases were estimated with qPCR, which showed that bacteria dominated the mica schist communities with a relatively high proportion of epilithic sulfate-reducing bacteria in all microcosms. The dominant bacterial phyla in the microcosms were Proteobacteria, Firmicutes, and Actinobacteria, whereas most fungal genera belonged to Ascomycota and Basidiomycota. Dissimilarities between planktic and sessile rock surface microbial communities were observed, and the supplied carbon substrates led to variations in the bacterial community composition.

show abstract

Section: Discussionmentioning

confidence: 99%

Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater

et al. 2023

View full text Add to dashboard Cite

show abstract

“…While calcium carbonate is mostly insoluble and thus unavailable as a carbon source, it has nevertheless been shown to support the growth of some serpentinite-hosted microorganisms. This might be the result of local redissolution into bicarbonate catalyzed by the carbonic anhydrase ( Suzuki et al, 2014 ; Fones et al, 2019 ; Bird et al, 2021 ) ( Table 1 ). Alternatively, small organic molecules may serve as primary source of carbon.…”

Section: Metabolic Strategies Of Serpentinite-hosted Ecosystemsmentioning

confidence: 99%

“…Transferred across the cell membrane via specialized transporters, formate can be oxidized to CO 2 via the formate dehydrogenase in the pH-neutral cytoplasm ( Brazelton et al, 2022 ). Likewise, bicarbonate can be reduced to CO 2 via the carbonic anhydrase ( Suzuki et al, 2014 ; Bird et al, 2021 ). The produced CO 2 is subsequently introduced to different carbon fixation pathways yielding acetyl-CoA.…”

Section: Metabolic Strategies Of Serpentinite-hosted Ecosystemsmentioning

confidence: 99%

“…This includes a major proportion of Serpentinimonas (formerly grouped under Betaproteobacteria). Serpentinimonas is one of the taxa most commonly associated with serpentinization, and represented by some of the few available isolates from serpentinite-hosted ecosystems ( Suzuki et al, 2014 ; Bird et al, 2021 ). So far, all of those isolated strains originate from The Cedars.…”

Section: Diversity Of Identified Metabolic Key Playersmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic challenges and key players in serpentinite-hosted microbial ecosystems

Popall¹,

Postec²,

Lecoeuvre³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Serpentinite-hosted systems are amongst the most challenging environments for life on Earth. Serpentinization, a geochemical alteration of exposed ultramafic rock, produces hydrothermal fluids enriched in abiotically derived hydrogen (H2), methane (CH4), and small organic molecules. The hyperalkaline pH of these fluids poses a great challenge for metabolic energy and nutrient acquisition, curbing the cellular membrane potential and limiting electron acceptor, carbon, and phosphorous availability. Nevertheless, serpentinization supports the growth of diverse microbial communities whose metabolic make-up might shed light on the beginning of life on Earth and potentially elsewhere. Here, we outline current hypotheses on metabolic energy production, carbon fixation, and nutrient acquisition in serpentinizing environments. A taxonomic survey is performed for each important metabolic function, highlighting potential key players such as H2 and CH4 cycling Serpentinimonas, Hydrogenophaga, Methanobacteriales, Methanosarcinales, and novel candidate phyla. Methodological biases of the available data and future approaches are discussed.

show abstract

“…Afterward, Macromonas bipunctata , another species of the genus Macromonas , was described by Dubinina and Grabovich [ 5 ] with a strain isolated from the precipitates of sewage aeration tanks. Due to the absence of available cultures and 16S rRNA gene sequences for M. mobilis , only Macromonas bipunctata has been designated as the type species of the genus for taxonomic classification [ 6 , 7 ]. The type strain Macromonas bipunctata IAM 14880 T exhibits characteristics such as being Gram-stain-negative, non-pigmented, oxidase-positive, catalase-negative, motile due to polar flagella, and aerobic.…”

Section: Introductionmentioning

confidence: 99%

Macromonas nakdongensis sp. nov., Isolated from Freshwater and Characterization of Bacteriophage BK-30P—The First Phage That Infects Genus Macromonas

Baek,

Choi

2023

Microorganisms

View full text Add to dashboard Cite

A Gram-stain-negative, non-motile, non-pigmented, rod-shaped bacterium was isolated from a freshwater sample of Nakdong River in South Korea and designated as strain BK-30T. An analysis of the 16S rRNA gene sequence of strain BK-30T revealed its closest phylogenetic neighbors were members of the genus Macromonas. Specifically, the strain formed a robust clade with Macromonas bipunctata DSM 12705T, sharing 98.4% similarity in their 16S rRNA gene sequences. The average nucleotide identity value between strain BK-30T and M. bipunctata DSM 12705T was 79.8%, and the genome-to-genome distance averaged 21.3%, indicating the representation of a novel genomic species. Strain BK-30T exhibited optimum growth at 30 °C and pH 7.0, in the absence of NaCl. The major respiratory isoprenoid quinone identified was ubiquinone-8 (Q-8). The principal fatty acids detected were C16:1 ω7c and/or C16:1 ω6c (49.6%), C16:0 (27.5%), and C18:1 ω7c and/or C18:1 ω6c (9.2%). The DNA G+C content of the strain was determined to be 67.3 mol%. Based on these data, we propose a novel species within the genus Macromonas, named Macromonas nakdongensis sp. nov., to accommodate the bacterial isolate. Strain BK-30T is designated as the type strain (=KCTC 52161T = JCM 31376T = FBCC-B1). Additionally, we present the isolation and complete genome sequence of a lytic phage infecting strain BK-30T, named BK-30P. This bacteriophage is the first reported to infect Macromonas, leading us to propose the name “Macromonasphage”.

show abstract

Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs

Cited by 25 publications

References 43 publications

Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater

Implications of a short carbon pulse on biofilm formation on mica schist in microcosms with deep crystalline bedrock groundwater

Metabolic challenges and key players in serpentinite-hosted microbial ecosystems

Macromonas nakdongensis sp. nov., Isolated from Freshwater and Characterization of Bacteriophage BK-30P—The First Phage That Infects Genus Macromonas

Contact Info

Product

Resources

About