Cyclic deformation of a superduplex stainless steel at temperatures ranging from room temperature to 475°C was evaluated for two different strain amplitudes. Cyclic hardening-softening response and the corresponding substructural features within each constituent phase of the alloy were characterized. Experimental evidence, such as abnormal cyclic hardening, inverse strain rate sensitivity (SRS), and serrated flow, reveals the existence of dynamic strain aging (DSA) in the studied temperature range. Substructural evolution suggests that DSA induces changes in the distribution of plastic strain between austenite and ferrite. In the case of tests performed at 475°C, there exists a significant influence of thermal embrittlement too.