Serratiopeptidase Niosomal Gel with Potential in Topical Delivery

Abstract

The objective of present study was to develop nonionic surfactant vesicles of proteolytic enzyme serratiopeptidase (SRP) by adapting reverse phase evaporation (REV) technique and to evaluate the viability of SRP niosomal gel in treating the topical inflammation. The feasibility of SRP niosomes by REV method using Span 40 and cholesterol has been successfully demonstrated in this investigation. The entrapment efficiency was found to be influenced by the molar ratio of Span 40 : cholesterol and concentration of SRP in noisome. The developed niosomes were characterized for morphology, particle size, and in vitro release. Niosomal gel was prepared by dispersing xanthan gum into optimized batch of SRP niosomes. Ex vivo permeation and in vivo anti-inflammatory efficacy of gel formulation were evaluated topically. SRP niosomes obtained were round in nanosize range. At Span 40 : cholesterol molar ratio 1 : 1 entrapment efficiency was maximum, that is, 54.82% ± 2.08, and showed consistent release pattern. Furthermore ex vivo skin permeation revealed that there was fourfold increase in a steady state flux when SRP was formulated in niosomes and a significant increase in the permeation of SRP, from SRP niosomal gel containing permeation enhancer. In vivo efficacy studies indicated that SRP niosomal gel had a comparable topical anti-inflammatory activity to that of dicolfenac gel.

Etodolac Containing Topical Niosomal Gel: Formulation Development and Evaluation

“…The right paw served as a reference of noninflamed paw for comparison. The percentage difference between right and left paw volumes was taken as percent edema produced (“as discussed by Shinde and Kanojiya [16]”). The percent edema produced with test samples was subtracted from percent edema produced in control group to obtain percent edema inhibition by respective groups.…”

Niosomes: a review of their structure, properties, methods of preparation, and medical applications

“…Therefore, a topical formulation of serratiopeptidase that potentially reduces adverse effects and increase local effects has been developed by Shinde and Kanojiya using niosomes. The results showed that the newly formulated serratiopeptidase niosomal gel had anti-inflammatory activity comparable to that of diclofenac gel [84].…”

Anti-Epileptic Drug Loaded Niosomal Transdermal Patch for Enhanced Skin Permeation

“…The stability studies of optimised midazolam loaded niosome (N5) and midazolam loaded niosomal transdermal patch (NT5) was done in the refrigerator (4±2 °C) and room temperature (30±2 °C)/65±5%RH for 90 d [94]. The optimised midazolam loaded niosome was subjected to particle size analysis which reviewed that there are no major variations in its stability in both room and refrigerator temperatures as illustrated in fig.…”