SERS Analysis of Bacteria, Human Blood, and Cancer Cells: a Metabolomic and Diagnostic Tool

Premasiri, W. Ranjith; Lemler, Paul M.; Chen, Y.; Gebregziabher, Yoseph; Ziegler, L. D.

doi:10.1002/9781118703601.ch12

Cited by 13 publications

(9 citation statements)

References 88 publications

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“…The spectral features evolved from an adenine-like species to the hypoxanthine-like species (The vertical black line at 736 cm –1 is to guide the identification of the Raman shift). These adenine-related species were secreted from cells as a consequence of the metabolic process and of the responses to the nutrient-free environments . Therefore, an improper detection environment could distort the native fingerprint information on living cells, making the identification of cancer cells and normal cells unreliable.…”

Section: Direct Bioanalytical Sers Detectionmentioning

confidence: 99%

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Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges

et al. 2018

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Surface-enhanced Raman spectroscopy (SERS) inherits the rich chemical fingerprint information on Raman spectroscopy and gains sensitivity by plasmon-enhanced excitation and scattering. In particular, most Raman peaks have a narrow width suitable for multiplex analysis, and the measurements can be conveniently made under ambient and aqueous conditions. These merits make SERS a very promising technique for studying complex biological systems, and SERS has attracted increasing interest in biorelated analysis. However, there are still great challenges that need to be addressed until it can be widely accepted by the biorelated communities, answer interesting biological questions, and solve fatal clinical problems. SERS applications in bioanalysis involve the complex interactions of plasmonic nanomaterials with biological systems and their environments. The reliability becomes the key issue of bioanalytical SERS in order to extract meaningful information from SERS data. This review provides a comprehensive overview of bioanalytical SERS with the main focus on the reliability issue. We first introduce the mechanism of SERS to guide the design of reliable SERS experiments with high detection sensitivity. We then introduce the current understanding of the interaction of nanomaterials with biological systems, mainly living cells, to guide the design of functionalized SERS nanoparticles for target detection. We further introduce the current status of label-free (direct) and labeled (indirect) SERS detections, for systems from biomolecules, to pathogens, to living cells, and we discuss the potential interferences from experimental design, measurement conditions, and data analysis. In the end, we give an outlook of the key challenges in bioanalytical SERS, including reproducibility, sensitivity, and spatial and time resolution.

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Section: Direct Bioanalytical Sers Detectionmentioning

confidence: 99%

Section: Direct Bioanalytical Sers Detectionmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges

et al. 2018

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“…For example, renal cells can start to secrete molecules in response to environmental conditions which may introduce complications in the reproducibility of the cell line's fingerprints. 310 Also, using PCA to detect differences in the spectra of membrane components between normal and cancerous cells can be quite complex. A simpler alternative for determination of cancer cells has been proposed by looking at the ratio of the bands 722/655 cm −1 .…”

Section: Cancer Cellsmentioning

confidence: 99%

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

2015

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“…Another major application advantage of such a tandem-SERS platform is to enrich the bacterial cells and subsequently improve the detection sensitivity. Although SERS can theoretically detect a single molecule/cell, its real world application can only detect ~10 3 CFU/mL of bacteria, mainly due to the interference from the sample matrix components [ 94 ]. Therefore, a relatively large amount of samples therefore is required for the production of a meaningful SERS signal readout.…”

Section: Tandem-sers For Sensing Bacteria In a Sample Matrixmentioning

confidence: 99%

Detection and Characterization of Antibiotic-Resistant Bacteria Using Surface-Enhanced Raman Spectroscopy

Wang

Petersen

et al. 2018

Nanomaterials

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This mini-review summarizes the most recent progress concerning the use of surface-enhanced Raman spectroscopy (SERS) for the detection and characterization of antibiotic-resistant bacteria. We first discussed the design and synthesis of various types of nanomaterials that can be used as the SERS-active substrates for biosensing trace levels of antibiotic-resistant bacteria. We then reviewed the tandem-SERS strategy of integrating a separation element/platform with SERS sensing to achieve the detection of antibiotic-resistant bacteria in the environmental, agri-food, and clinical samples. Finally, we demonstrated the application of using SERS to investigate bacterial antibiotic resistance and susceptibility as well as the working mechanism of antibiotics based on spectral fingerprinting of the whole cells.

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SERS Analysis of Bacteria, Human Blood, and Cancer Cells: a Metabolomic and Diagnostic Tool

Cited by 13 publications

References 88 publications

Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges

Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges

SERS Nanoparticles in Medicine: From Label-Free Detection to Spectroscopic Tagging

Detection and Characterization of Antibiotic-Resistant Bacteria Using Surface-Enhanced Raman Spectroscopy

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