SERS and Cryo-EM Directly Reveal Different Liposome Structures during Interaction with Gold Nanoparticles

Živanović, Vesna; Kochovski, Zdravko; Lü, Yan; Kneipp, Janina

doi:10.1021/acs.jpclett.8b03191

Cited by 39 publications

(85 citation statements)

References 46 publications

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“…42 In these cells, a few spectra also show signals at the typical frequencies of the lipid ester C=O stretching vibration around 1740 cm -1 , and most bands associated with C-H deformation vibrations have a Raman shift of 1432 cm -1 or of 1440 cm -1 characteristic of CH 2 groups that could be part of long lipid chains ( Figure 5E). 67 The interaction of the nanostars with a lipid rich environment is also visible in the characteristic Raman shift of the CH 2 deformation of lipids that occurs around 1365 cm -1 ( Figure 5F) or the =C-H deformation around 1273 cm -1 . 67 Such interfacial interactions can be related to the processing of gold nanoparticles by the cells, 38 since the protein corona plays an important role as mediator between the pristine nanostructure and the cellular environment.…”

Section: Biomolecular Interactions At the Nanostar Surface In Living mentioning

confidence: 91%

Intracellular optical probing with gold nanostars

et al. 2021

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Section: Biomolecular Interactions At the Nanostar Surface In Living mentioning

confidence: 91%

Intracellular optical probing with gold nanostars

et al. 2021

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“…Probing of intrinsic cellular structures without reporter molecules using lipid coated Au@BaTiO 3 particles in macrophage cells is presented in Figure E. The SERS spectra show lipid bands due to the lipid coating of the nanoprobes or of cellular membranes, and also bands that are characteristic of other molecules in the cells. For example, while the band at 714 cm −1 , present in most of the spectra, is very characteristic for the C–N stretching of the choline group in lipids, the bands at 827 and 853 cm −1 can be assigned to aromatic amino acids with high Raman cross sections that are abundant in proteins .…”

Section: Resultsmentioning

confidence: 99%

Gold‐ and Silver‐Coated Barium Titanate Nanocomposites as Probes for Two‐Photon Multimodal Microspectroscopy

Madzharova

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Živanović

et al. 2019

Adv Funct Materials

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Improved multiphoton-excited imaging and microspectroscopy require nanoprobes that can give different nonlinear optical signals. Here, composite nanostructures with a barium titanate core and a plasmonic moiety at their surface are synthesized and characterized. It is found that the core provides a high second-order nonlinear susceptibility for sensitive second harmonic generation (SHG) imaging in living cells. As a second function in the twophoton regime, the plasmonic part yields high local fields for resonant and nonresonant surface enhanced hyper Raman scattering (SEHRS). SEHRS complements the one-photon surface enhanced Raman scattering (SERS) spectra that are also enhanced by the plasmonic shells. Barium titanate silver core-shell (Ag@BaTiO 3 ) composites are specifically suited for SEHRS and SHG excited at 1064 nm, while gold at barium titanate (Au@BaTiO 3 ) nanoparticles can be useful in a combination of SHG and SERS at lower wavelengths, here at 785 nm and 850 nm. The theoretical models show that the optical properties of the BaTiO 3 dielectric core depend on probing frequency, shape, size, and plasmonic properties of the surrounding gold nanoparticles so that they can be optimized for a particular type of experiment. These versatile, tunable probes give new opportunities for combined multiphoton probing of morphological structure and chemical properties of biosystems.very attractive in laser microspectroscopy and imaging. They include two-photonexcited fluorescence, [3] second harmonic generation (SHG), [4] stimulated Raman scattering [5] and coherent anti-Stokes Raman scattering [6] microscopies, and imaging based on spontaneous hyper Raman scattering (HRS). [7] Nonlinear processes are attractive in microscopy and spectroscopy since they can be excited with light in the near-infrared, which offers several advantages. The latter include deep tissue penetration capability, reduced photo damage due to the lower photon energy, and spatially more confined probed volume, which can result in an improved lateral resolution. [8] Nevertheless, when multiphoton-excited processes are complemented with their one-photon-excited counterparts, this can yield unprecedented structural information from a sample. As an example, the spontaneous twophoton-excited Raman process, termed HRS, is governed by different selection rules than the (conventional) spontaneous one-photon-excited RS. Both HRS and RS can be enhanced in the local fields of plasmonic nanostructures, resulting in surface enhanced hyper Raman scattering (SEHRS) [9] and surface enhanced Raman scattering (SERS) spectra, respectively. While imaging based on vibrational spectra gives detailed molecular structure, other nonlinear optical signals, such as SHG, are less specific but lead to information about the microscopic morphology of a sample. SHG is a two-photon process, where a

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“…Živanović and co‐workers presented structural information on liposomes at the site of interaction with AuNPs by using the SERS method (Figure B) . Knowing the interaction of NPs and liposomes can be advantageous in drug delivery and curing infected cells .…”

Section: Sers‐based Biosensorsmentioning

confidence: 99%

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

et al. 2019

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Label‐free biosensors offer accurate sensing capabilities due to the reliable quantification of biological and biochemical processes. These devices function by establishing a dynamic interaction of analyte and receptor molecules and convert this interaction into a measurable signal through a transducer. In recent decades, label‐free biosensors have attracted attention in biomedical applications due to the ease of linking nanomaterials with bioreceptor molecules. In this review, recent advances in sensitivity, specificity, and sensing mechanism related to label‐free biosensors of metallic nanoparticles of gold, silver, aluminium, copper, and zinc oxide are presented. Selected sensing methods based on fluorescence, surface plasmon resonance, surface‐enhanced Raman scattering, metal‐enhanced fluorescence, and electrochemical sensors are discussed. New measurement techniques and rapid progress of label‐free biosensors are going to play a vital role in the real‐time detection of biomarkers in clinical samples, such as blood plasma, serum, and urine, as well as in targeted drug delivery. Future trends of these label‐free biosensing mechanisms and their development are also discussed.

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SERS and Cryo-EM Directly Reveal Different Liposome Structures during Interaction with Gold Nanoparticles

Cited by 39 publications

References 46 publications

Intracellular optical probing with gold nanostars

Intracellular optical probing with gold nanostars

Gold‐ and Silver‐Coated Barium Titanate Nanocomposites as Probes for Two‐Photon Multimodal Microspectroscopy

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

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