SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation

Moisoiu, Vlad; Ştefancu, Andrei; Iancu, Ștefania D.; Moisoiu, Tudor; Loga, Luminița; Dican, Lucia; Alecsa, Cristian Daniel; Boros, Imre; Jurj, Anca; Dima, Delia; Bagacean, Cristina; Tetean, R.; Burzo, Emil; Tomuleasa, Ciprian; Elec, Florin Ioan; Leopold, Nicolae

doi:10.1007/s00216-019-02213-2

Cited by 30 publications

(41 citation statements)

References 17 publications

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“…It is well known that cancer DNA has a tendency towards hypomethylation. When DNA was extracted from an acute myeloid leukemia (AML) cell line, we found a decreased intensity of the 1005 cm -1 band of 5-methylcytosine compared to normal DNA, determined by the hypomethylation of the AML cells (31). This finding was then further tested on peripheral blood genomic DNA samples, yielding a high discriminative accuracy (82%).…”

Section: Tumor Biomarkers From Body Fluids Targeted With Sersmentioning

confidence: 82%

“…Recent fundamental studies indicate that the SERS spectra of cationic and anionic analytes are switched on in a specific manner by the type of adsorbed ion (adion) (28)(29)(30), the role of adions such as Ag + , Ca 2+ , Mg 2+ , Cl -, Br -, I -, being explained in the frame of the proposed adion-specific adsorption model (28). Thus, the SERS spectra of uric acid, xanthine and hypoxanthine purine metabolites are enhanced after activation of silver nanoparticles with cationic analytes such as Ca 2+ or Mg 2+ (11,12), whereas albumin and DNA detection require the activation of silver nanoparticles with Iand Cl -, respectively (27,31).…”

Section: Methods and Samplesmentioning

confidence: 99%

“…Recently, we have shown the correlation between DNA methylation pattern and SERS spectra, shedding light on a potential new method for cancer diagnosis (31,61). It is well known that cancer DNA has a tendency towards hypomethylation.…”

Section: Tumor Biomarkers From Body Fluids Targeted With Sersmentioning

confidence: 95%

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Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

et al. 2020

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As colorectal cancer (CRC) is one of the forms of cancer with the highest prevalence globally and with a high mortality, screening and early detection remains a major issue. Colonoscopy is still the gold standard for detecting premalignant lesions, but it is burdened by some complications. For instance, it is laborious, with some difficulties of acceptance for some patients, and is ultimately an imperfect standard, given that some premalignant lesions or incipient malignancies can be missed by colonoscopic evaluation. In this context, new non-invasive approaches such as surface-enhanced Raman spectroscopy (SERS) based liquid biopsy have gained ground in recent years, showing promising results in oncological pathology diagnosis. These new methods have enabled the detection of subtle molecular profile alterations prior to any macroscopic morphological changes, thus providing a useful tool for early CRC detection. In the present review, we provide a summary of published studies applying SERS in CRC detection, along with our personal experience in using SERS in the diagnosis of different oncological pathologies, including CRC.

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Section: Tumor Biomarkers From Body Fluids Targeted With Sersmentioning

confidence: 82%

Section: Methods and Samplesmentioning

confidence: 99%

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Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

et al. 2020

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“…Raman Spectroscopy is a non-destructive form of optical spectroscopy that relies on the interaction of light with the vibrational energy structure of chemical bonds of molecules and detects the inelastic scattering of laser photons. Subsequently, the frequency-shifted photons provide detailed information about the molecular structure of the sample ( 76 , 77 ).…”

Section: Surface-enhanced Raman Scattering (Sers) As An Innovative Hmentioning

confidence: 99%

“…Thus, its low sensitivity often limits its use. To overcome this challenge and enhance the sensitivity of the method, Surface-enhanced Raman scattering (SERS), a technique that involves metal nanostructures for the amplification of the Raman signal of molecules, has been developed ( 76 , 78 ). When the target of interest adsorbs onto nanometer-sized metal substrates such as silver or gold colloidal nanoparticles, the Raman signal of the target molecule is dramatically augmented ( 77 , 79 ).…”

Section: Surface-enhanced Raman Scattering (Sers) As An Innovative Hmentioning

confidence: 99%

SERS-Based Assessment of MRD in Acute Promyelocytic Leukemia?

et al. 2020

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Acute promyelocytic leukemia (APL) is characterized by a unique chromosome translocation t(15;17)(q24;q21), which leads to the PML/RARA gene fusion formation. However, it is acknowledged that this rearrangement alone is not able to induce the whole leukemic phenotype. In addition, epigenetic processes, such as DNA methylation, may play a crucial role in leukemia pathogenesis. DNA methylation, catalyzed by DNA methyltransferases (DNMTs), involves the covalent transfer of a methyl group (-CH3) to the fifth carbon of the cytosine ring in the CpG dinucleotide and results in the formation of 5-methylcytosine (5-mC). The aberrant gene promoter methylation can be an alternative mechanism of tumor suppressor gene inactivation. Understanding cancer epigenetics and its pivotal role in oncogenesis, can offer us not only attractive targets for epigenetic treatment but can also provide powerful tools in monitoring the disease and estimating the prognosis. Several genes of interest, such as RARA, RARB, p15, p16, have been studied in APL and their methylation status was correlated with potential diagnostic and prognostic significance. In the present manuscript we comprehensively examine the current knowledge regarding DNA methylation in APL pathogenesis. We also discuss the perspectives of using the DNA methylation patterns as reliable biomarkers for measurable residual disease (MRD) monitoring and as a predictor of relapse. This work also highlights the possibility of detecting aberrant methylation profiles of circulating tumor DNA (ctDNA) through liquid biopsies, using the conventional methods, such as methylation-specific polymerase chain reaction (MS-PCR), sequencing methods, but also revolutionary methods, such as surface-enhanced Raman spectroscopy (SERS).

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Discernable machine learning methods for Raman micro‐spectroscopic stratification of mitoxantrone‐induced drug‐resistant cells in acute myeloid leukemia

Anjikar,

Iwasaki,

Paneerselvam

et al. 2024

J Raman Spectroscopy

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Drug resistance plays a vital role in both cancer treatment and prognosis. Especially, early insights into such drug‐induced resistance in acute myeloid leukemia (AML) can help to improve treatment plans, reduce costs, and bring overall positive outcomes for patients. Raman spectroscopy provides precise biomolecular information and can provide all these necessities effectively. In this study, we employed machine learning (ML) discrimination of Raman micro‐spectroscopic data of myelocytic leukemia cell line HL‐60 from its drug‐resistant counterpart HL‐60/MX2. Principal component analysis (PCA), linear discriminant analysis (LDA), and logistic regression (LR) methods were evaluated for their ability to identify and discriminate drug resistance in AML cells. Our study demonstrates the power of ML to classify drug‐induced resistance in AML cells utilizing subtle variations in biomolecular information contained in molecular spectroscopic data by obtaining 94.11% and 97.05% classification accuracies by LDA and LR models, respectively. We also showed that the ML methods are discernable. Our findings depict the importance of automation and its optimal usage in cancer study and diagnosis. The results of our study are expected to take ML‐assisted Raman spectroscopy one step closer to making it a generalized tool in medical diagnosis in the future.

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SERS assessment of the cancer-specific methylation pattern of genomic DNA: towards the detection of acute myeloid leukemia in patients undergoing hematopoietic stem cell transplantation

Cited by 30 publications

References 17 publications

Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

SERS-Based Assessment of MRD in Acute Promyelocytic Leukemia?

Discernable machine learning methods for Raman micro‐spectroscopic stratification of mitoxantrone‐induced drug‐resistant cells in acute myeloid leukemia

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