SERS Blinking on Anisotropic Nanoparticles

Wrzosek, Beata; Kitahama, Yasutaka; Ozaki, Yukihiro

doi:10.1021/acs.jpcc.0c03445

Cited by 10 publications

(7 citation statements)

References 78 publications

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“…Anisotropic plasmonic nanostructures may exhibit SERS blinking, which can affect the stability and reproducibility of the signal. 76 In addition, the optical response of anisotropic nanostructures may vary, impacting the uniformity of Raman enhancement.…”

Section: Anisotropic Plasmonic Nanostructuresmentioning

confidence: 99%

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Ranasinghe,

Wang,

Huang

2024

Nanoscale

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Section: Anisotropic Plasmonic Nanostructuresmentioning

confidence: 99%

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Ranasinghe,

Wang,

Huang

2024

Nanoscale

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“…Since the SERS spectra notoriously variate [44], we used the following protocol. We acquired each spectrum for 30 s to average the blinking of the spectral signals of the molecules, performing a kind of "random walk" on the metal surface [45], especially in the absence of a functionalization. Indeed, the blinking mechanism usually ranges in a timescale of seconds [46].…”

Section: Raman and Sers Measurementsmentioning

confidence: 99%

Molecular Fingerprinting of the Omicron Variant Genome of SARS-CoV-2 by SERS Spectroscopy

et al. 2022

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The continuing accumulation of mutations in the RNA genome of the SARS-CoV-2 virus generates an endless succession of highly contagious variants that cause concern around the world due to their antibody resistance and the failure of current diagnostic techniques to detect them in a timely manner. Raman spectroscopy represents a promising alternative to variants detection and recognition techniques, thanks to its ability to provide a characteristic spectral fingerprint of the biological samples examined under all circumstances. In this work we exploit the surface-enhanced Raman scattering (SERS) properties of a silver dendrite layer to explore, for the first time to our knowledge, the distinctive features of the Omicron variant genome. We obtain a complex spectral signal of the Omicron variant genome where the fingerprints of nucleobases in nucleosides are clearly unveiled and assigned in detail. Furthermore, the fractal SERS layer offers the presence of confined spatial regions in which the analyte remains trapped under hydration conditions. This opens up the prospects for a prompt spectral identification of the genome in its physiological habitat and for a study on its activity and variability.

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“…These localized SERS hotspots are much smaller than the wavelength of the illuminating light, perhaps approaching atomic dimensions. , The resonance behavior of these hotspots depends highly on the local nanoenvironment. With such nanofocused light, single-molecule behavior is readily observed, , with one of the hallmarks being fluctuations in the overall SERS intensity and spectral features. − Recent experiments done with extremely high-speed imaging revealed that the fluctuations occur over a wide range of time-scales, from tens of seconds down to single microseconds. , Super-resolution imaging and experiments with multiple laser wavelengths and polarization states showed that SIFs occur on the subnanoparticle level in hyper-localized (<10 nm) regions of the larger nanoparticle (∼100 nm). These experiments suggest that intensity fluctuations, which occur in different combinations of probe molecule and nanoparticle shape, size, and material, are a universal and fundamental characteristic of the SERS effect that becomes more and more relevant with high-speed or single-molecule experiments.…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule SERS Hotspot Dynamics in Both Dry and Aqueous Environments

2022

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Fluctuations in Surface Enhanced Raman Spectroscopy (SERS) signals are a hallmark of few-to-single molecule events. Recent experiments have shown these SERS intensity fluctuations (SIFs) to occur over an extremely wide range of time scales, from seconds to microseconds. While many mechanisms have been proposed, such as molecular diffusion or transient plasmonic hotspot generation, the underlying source of these fluctuations is likely to be a complex interplay of different effects. For example, SERS experiments done with fully coated, but dry, nanoparticles would have less movement of the probe molecule but may still have transient hotspot generation due to the mobility of metallic atoms. Alternatively, experiments done with low concentrations of a molecular probe in liquid might tend to see fluctuations caused by freely diffusing molecules that visit and become trapped within a static hotspot. In this paper we compare high-speed SIF activity in both dry and wet nanoparticle environments. By carefully analyzing the overall SERS signal fluctuations, such as the timing statistics, we propose a simple model that accounts for the contributions from the various mechanisms discussed above. These results provide a deeper physical understanding of the SERS effect and may promote further research into singlemolecule SERS, its experimental optimization, and potential applications.

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SERS Blinking on Anisotropic Nanoparticles

Cited by 10 publications

References 78 publications

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Unveiling brain disorders using liquid biopsy and Raman spectroscopy

Molecular Fingerprinting of the Omicron Variant Genome of SARS-CoV-2 by SERS Spectroscopy

Single-Molecule SERS Hotspot Dynamics in Both Dry and Aqueous Environments

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