SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates

Andrei, Cristina-Cassiana; Moraillon, A.; Larquet, Éric; Potara, Monica; Aştilean, Simion; Jakab, Endre; Bouckaert, Julie; Rosselle, Léa; Skandrani, Nadia; Boukherroub, Rabah; Ozanam, F.; Szunerits, Sabine; Gouget-Laemmel, Anne Chantal

doi:10.1007/s00216-020-03106-5

Cited by 20 publications

(12 citation statements)

References 62 publications

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“…As a result, the interest in SERS detection and identification of biological samples such as proteins, viruses, fungal cells, and cancer cells has increased significantly. − Moreover, numerous scientific publications have proven that SERS can be applied for reliable identification and detection of various bacterial strains. − This is an important issue, especially when considering bacterial pathogens responsible for different human diseases.…”

mentioning

confidence: 99%

In Search of Spectroscopic Signatures of Periodontitis: A SERS-Based Magnetomicrofluidic Sensor for Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans

et al. 2021

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Recently, Porphyromonas gingivalis, the keystone pathogen implicated in the development of gum disease (periodontitis), was detected in the brains of Alzheimer's disease patients, opening up a fascinating possibility that it is also involved in the pathobiology of this neurodegenerative illness. To verify this hypothesis, an unbiased, specific, and sensitive method to detect this pathogen in biological specimens is needed. To this end, our interdisciplinary studies demonstrate that P. gingivalis can be easily identified by surface-enhanced Raman scattering (SERS). Moreover, based on SERS measurements, P. gingivalis can be distinguished from another common periodontal pathogen, Aggregatibacter actinomycetemcomitans, and also from ubiquitous oral Streptococcus spp. The results were confirmed by principal component analysis (PCA). Furthermore, we have shown that different P. gingivalis and A. actinomycetemcomitans strains can easily adsorb to silver-coated magnetic nanoparticles (Fe 2 O 3 @AgNPs). Thus, it is possible to magnetically separate investigated bacteria from other components of a specimen using the microfluidic chip. To obtain additional enhancement of the Raman signal, the NPs adsorbed to bacterial cells were magnetically attracted to the Si/Ag SERS platform. Afterward, the SERS spectra could be recorded. Such a time-saving procedure can be very helpful in rapid medical diagnostics and thus in starting the appropriate pharmacological therapy to prevent the development of periodontitis and associated comorbidities, e.g., Alzheimerʼs disease.

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confidence: 99%

In Search of Spectroscopic Signatures of Periodontitis: A SERS-Based Magnetomicrofluidic Sensor for Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans

et al. 2021

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“…The SERS spectroscopy has been widely used for analyzing various biosamples, including DNA (Pyrak et al, 2019;Zhang et al, 2019a), RNA (Lee et al, 2019;Han et al, 2021), cancer markers (Choi et al, 2020), bacteria (Andrei et al, 2021), viruses (Chen et al, 2020), genes (Vo-Dinh et al, 2002), drugs (Jaworska et al, 2016), pathological markers on cellular membranes and tissues (Wallace and Masson, 2020), other biomolecules, ion concentrations, and redox potential in cells (Jaworska et al, 2021), and even in vivo SERS measurements on mice (Wen et al, 2021). In many cases, the results obtained are at a level of detection not achievable by other analytical methods.…”

Section: Surface-enhanced Raman Scattering For (Bio)medical Applicationsmentioning

confidence: 99%

Applications of Surface-Enhanced Raman Scattering in Biochemical and Medical Analysis

Szaniawska

Kudelski

2021

Front. Chem.

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In this mini-review, we briefly describe certain recently developed applications of the surface-enhanced Raman spectroscopy (SERS) for determining various biochemically (especially medically) important species from ones as simple as hydrogen cations to those as complex as specific DNA fragments. We present a SERS analysis of species whose characterization is important to our understanding of various mechanisms in the human body and to show its potential as an alternative for methods routinely used in diagnostics and clinics. Furthermore, we explain how such SERS-based sensors operate and point out future prospects in this field.

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“…The surface enhancement principle relies on the hot spots created between gold nanoparticles. , The classical SERS active substrate is gold nanoparticles, which are easy to prepare and have good biocompatibility and unique physical compatibility. − However, a SERS-based biosensor has low selectivity for the detection of pathogens. Most reported SERS tags used for the detection of pathogens are absorbed on the surface of nanoparticles, which were easy to eliminate and destroy in the washing process. − The specific recognition ability of the detector is not enough, which is likely to lead to false positive and false negative causalities. To solve this problem, the introduction of aptamers on a SERS biosensor is an effective strategy.…”

Section: Introductionmentioning

confidence: 99%

Trace Detection of E. coli O157:H7 Cells by an Au Nanoparticle-Based SERS Aptasensor

Tian

Zhao

et al. 2023

ACS Appl. Nano Mater.

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Drinking polluted water can cause deadly diseases, and clean water is becoming increasingly scarce in the environment. Various pathogens in water will affect environmental safety and human health, so it is necessary to detect pathogens with sensitivity, specificity, simplicity, and celerity. Here, we designed a surface-enhanced Raman scattering (SERS) adaptive sensor based on rolling cycle amplification (RCA), in which the introduced Au nanoparticle-based SERS aptasensor could transform the concentration signal of Escherichia coli into a Raman signal, realizing the sensitive analysis of E. coli O157:H7 (E. coli O157:H7). In this strategy, the magnetic beads were modified with double-stranded DNA (dsDNA) to recognize E. coli and initiate RCA. In the presence of E. coli, one strand of dsDNA will recognize it and unchained from dsDNA and the other strand of dsDNA will act as a trigger for RCA. Then, the RCA reaction was initiated. A large number of SERS probes can be attached to RCA products to achieve a strong Raman signal. Under the optimal conditions, we obtained a low limit of detection (LOD = 0.3 CFU/mL) and a wide detection range (102–107 CFU/mL). Also, this SERS aptasensor can complete the detection within 45 min. It can be concluded that this SERS aptasensor has wide application prospects in food safety inspections, environmental monitoring, and clinical diagnosis, as it can provide a fast, sensitive, and one-pot platform for bacterial detection.

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SERS characterization of aggregated and isolated bacteria deposited on silver-based substrates

Cited by 20 publications

References 62 publications

In Search of Spectroscopic Signatures of Periodontitis: A SERS-Based Magnetomicrofluidic Sensor for Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans

In Search of Spectroscopic Signatures of Periodontitis: A SERS-Based Magnetomicrofluidic Sensor for Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans

Applications of Surface-Enhanced Raman Scattering in Biochemical and Medical Analysis

Trace Detection of E. coli O157:H7 Cells by an Au Nanoparticle-Based SERS Aptasensor

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