SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate

Lemma, Tibebe; Saliniemi, Alex; Hynninen, Ville; Hytönen, Vesa P.; Toppari, J. Jussi

doi:10.1016/j.vibspec.2016.01.006

Cited by 51 publications

(51 citation statements)

References 27 publications

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“…[25,26] In this respect, different types of SERS-substrates have been developed to obtain plasmon enhancement and record Raman spectra from (sub)cellular components down to the single biomolecule level. [27] These can be, but are not limited to, well defined nanostructured surfaces of gold [28] or silver [29] and (intracellular) aggregated Ag [30] or AuNP [31] . Both Ag-nanograin coated chips and precipitated AuNP clusters were recently applied for bulk EV measurements.…”

Section: Introductionmentioning

confidence: 99%

Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

Stremersch

Marro

Pinchasik

et al. 2016

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Exosome-like vesicles (ELVs) are a novel class of biomarkers that are receiving a lot of attention for the detection of cancer in an early stage. In this study the feasibility of using a Surface Enhanced Raman Spectroscopy (SERS) based method to distinguish between ELVs derived from different cellular origins is evaluated. A gold nanoparticle based shell is deposited on the surface of ELVs derived from cancerous and healthy cells which enhances the Raman signal while maintaining a colloidal suspension of individual vesicles. This nano-coating allows the recording of SERS spectra from single vesicles. By using Partial Least Square Discriminative Analysis (PLS-DA) on the obtained spectra, vesicles from different origin can be distinguished, even when present in the same mixture. This proof-of-concept study paves the way for non-invasive (cancer) diagnostic tools based on exosomal SERS fingerprinting in combination with multivariate statistical analysis.

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Section: Introductionmentioning

confidence: 99%

Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

Stremersch

Marro

Pinchasik

et al. 2016

Small

160

167

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“…The chemical composition of the cell content and cell surface are closely related with respect to the cell state. Therefore, the analysis of cell lysate and cell wall can provide valuable information. This is a very difficult task and at present mainly simple approaches are applied where the cell lysate is introduced to NP suspension directly on the chip, without any extensive sample pretreatment.…”

Section: Current Application Trendsmentioning

confidence: 99%

“…For example, the group of Popp was able to distinguish six mycobacteria species , whereas Lemma et al. designed a system for recognition of gram‐positive and gram‐negative bacteria . Similar approaches are used also for investigation of cell walls.…”

Section: Current Application Trendsmentioning

confidence: 99%

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Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

2017

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Surface-enhanced Raman spectroscopy (SERS) is an extremely powerful analytical tool, which not only yields information about the molecular structure of the analyte in the form of characteristic vibrational spectrum but also gives sensitivities approaching those in fluorescence spectroscopy. The SERS measurement on the microfluidic platform provides possibility to manufacture the device with design perfectly fulfilling the needs of the application with minimal sample consumption. This review aims at describing basic strategies for SERS measurement in microfluidic devices published in the last decade and covers current trends in microfluidics with SERS detection in the field of bioanalysis and approaches toward on-line coupling of liquid-based separation techniques with SERS detection.

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“…The development of sensitive techniques for the detection and quantitative analysis of biomolecules is important for trace element detection, environmental monitoring, and early stage diagnosis and treatment of diseases. Detection methods include high‐performance liquid chromatography, liquid chromatography mass spectrometry, and enzyme‐linked immunosorbent assays . A limit of detection of 1 ng/ml has been achieved with these platforms, a sensitivity that is among the best currently achievable in biological sensing.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform

Hayden

Wang

et al. 2019

J Raman Spectroscopy

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Surface enhanced Raman spectroscopy (SERS) holds great promise in biosensing because of its single‐molecule, label‐free sensitivity. We describe here the use of a graphene–gold hybrid plasmonic platform that enables quantitative SERS measurement. Quantification is enabled by normalizing analyte peak intensities to that of the graphene G peak. We show that two complementary quantification modes are intrinsic features of the platform and that through their combined use, the platform enables accurate determination of analyte concentration over a concentration range spanning seven orders of magnitude. We demonstrate, using a biologically relevant test analyte, the amyloid β‐protein (Aβ), a seminal pathologic agent of Alzheimer's disease, that linear relationships exist between (a) peak intensity and concentration at a single plasmonic hot spot smaller than 100 nm and (b) frequency of hot spots with observable protein signals, that is, the colocation of an Aβ protein and a hot spot. We demonstrate the detection of Aβ at a concentration as low as 10−18 M after a single 20 μl aliquot of the analyte onto the hybrid platform. This detection sensitivity can be improved further through multiple applications of analyte to the platform and by rastering the laser beam with smaller step sizes.

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SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate

Cited by 51 publications

References 27 publications

Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

Recent strategies toward microfluidic‐based surface‐enhanced Raman spectroscopy

Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform

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