SERS detection of hepatitis B virus DNA in a temperature‐responsive sandwich‐hybridization assay

Zengi̇n, Adem; Tamer, Uğur; Çaykara, Tuncer

doi:10.1002/jrs.5109

Cited by 44 publications

(23 citation statements)

References 22 publications

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“…However, EM is certainly inappropriate for routine clinical diagnosis because of the required time and cost, as well as many safety concerns. Furthermore, although the simple structure of virus particles allows the features of diagnostic relevance to be easily defined and tested, it presents limitations on the use of their characteristics for practical applications, especially with the increasing number of discovered viruses and recorded viral infections 14 , 16 , 17 . In addition, this structural simplicity confers viruses with rapid rates of spontaneous adaptation and evolution that may occur through direct genetic mutation, genetic substitution, or recombination.…”

Section: Viruses: Nanoscale Pathogens With a Global Burdenmentioning

confidence: 99%

Applications of gold nanoparticles in virus detection

2018

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Viruses are the smallest known microbes, yet they cause the most significant losses in human health. Most of the time, the best-known cure for viruses is the innate immunological defense system of the host; otherwise, the initial prevention of viral infection is the only alternative. Therefore, diagnosis is the primary strategy toward the overarching goal of virus control and elimination. The introduction of a new class of nanoscale materials with multiple unique properties and functions has sparked a series of breakthrough applications. Gold nanoparticles (AuNPs) are widely reported to guide an impressive resurgence in biomedical and diagnostic applications. Here, we review the applications of AuNPs in virus testing and detection. The developed AuNP-based detection techniques are reported for various groups of clinically relevant viruses with a special focus on the applied types of bio-AuNP hybrid structures, virus detection targets, and assay modalities and formats. We pay particular attention to highlighting the functional role and activity of each core Au nanostructure and the resultant detection improvements in terms of sensitivity, detection range, and time. In addition, we provide a general summary of the contributions of AuNPs to the mainstream methods of virus detection, technical measures, and recommendations required in guidance toward commercial in-field applications.

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Section: Viruses: Nanoscale Pathogens With a Global Burdenmentioning

confidence: 99%

Applications of gold nanoparticles in virus detection

2018

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“…This high enhancement makes it possible to detect DNA at concentrations of about 1 fM and a one-base mismatch. Silicon was also used in a SERS substrate in the form of Si-poly(MeO 2 MA)-SH@Au in a "sandwich" sensor for the detection of the hepatitis B virus DNA [75], and as silica wafers decorated with silver nanoparticles [76].…”

Section: Sandwich Sensors With Rr Connected To Dnamentioning

confidence: 99%

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

et al. 2019

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A sensitive and accurate identification of specific DNA fragments (usually containing a mutation) can influence clinical decisions. Standard methods routinely used for this type of detection are PCR (Polymerase Chain Reaction, and its modifications), and, less commonly, NGS (Next Generation Sequencing). However, these methods are quite complicated, requiring time-consuming, multi-stage sample preparation, and specially trained staff. Usually, it takes weeks for patients to obtain their results. Therefore, different DNA sensors are being intensively developed by many groups. One technique often used to obtain an analytical signal from DNA sensors is Raman spectroscopy. Its modification, surface-enhanced Raman spectroscopy (SERS), is especially useful for practical analytical applications due to its extra low limit of detection. SERS takes advantage of the strong increase in the efficiency of Raman signal generation caused by a local electric field enhancement near plasmonic (typically gold and silver) nanostructures. In this condensed review, we describe the most important types of SERS-based nanosensors for genetic studies and comment on their potential for becoming diagnostic tools.

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“…Zengin, Tamer, and Caykara reported SERS detection of hepatitis B virus DNA in a temperature‐responsive sandwich‐hybridization assay. They expect this highly sensitive and robust hybrid silicon substrate‐based SERS platform can be extended to detect other biomolecules and biochemical species such as viruses, proteins, and small molecules without any labeling …”

Section: Biosciencesmentioning

confidence: 99%

“…They expect this highly sensitive and robust hybrid silicon substratebased SERS platform can be extended to detect other biomolecules and biochemical species such as viruses, proteins, and small molecules without any labeling. [78] 11 | NANNOMATERIALS Nanomaterials, including fullerenes, nanotubes, graphene, and nanosized particles, represents an exciting area of growth and application for a variety of forms of Raman spectroscopy. The papers highlighted here have their principal emphasis on these materials, but additional papers relating to nanomaterials can be found in other sections of this review classified primarily by, for example, SERS.…”

Section: Medical and Biomedical Applications Tissues Plants And mentioning

confidence: 99%

Recent advances in linear and nonlinear Raman spectroscopy. Part XII

Nafie

2018

J Raman Spectroscopy

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This annual review is an overview of advances in the field of Raman spectroscopy as found in papers published in the previous calendar year in the Journal of Raman Spectroscopy (JRS), as well as in trends over the past decade across journals that have published papers important to the field of Raman spectroscopy. This information is gleaned from statistical data on article counts obtained from the Clarivate Analytic's Web of Science Core Collection by year and by subfield of Raman spectroscopy. Additional information is gleaned from presentations at the XXVI International Conference on Raman Spectroscopy (ICORS 2018) in Jeju Island, Korea in August 2018, and contributions on Raman scattering at SCIX 2018, organized by the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) in Atlanta, Georgia, USA in October 2018. Coverage is also provided for topics from the European Conference on Nonlinear Optical Spetroscopy (ECONOS 2018) held in April in Milan, Italy and GeoRaman 2018 in Catania, Italy in June. Finally, papers published in JRS in 2017 are highlighted and arranged by topics at the frontier of Raman spectroscopy. On the basis of this survey information, it is clear that Raman spectroscopy continues as in recent years as a rapidly expanding field of research across a wide range of novel disciplines and applications that provide sensitive photonic information of matter at the molecular level.

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SERS detection of hepatitis B virus DNA in a temperature‐responsive sandwich‐hybridization assay

Cited by 44 publications

References 22 publications

Applications of gold nanoparticles in virus detection

Applications of gold nanoparticles in virus detection

Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Recent advances in linear and nonlinear Raman spectroscopy. Part XII

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