SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A

SERS-Based Colloidal Aptasensors for Quantitative Determination of Influenza Virus

“…SERS-based assays with the highest performance consume solid nanostructured substrates with complex surfaces [3]. For example, aptamer-covered multilayer substrates made up of polyethylene naphthalate, gold, perfluorodecanethiol and one more gold layer were used to detect influenza A virus with a limit of detection of nearly 100 pfu/mL (10 4 VP/mL [30]) [13]. This technique was shown to be useful for virus quantitation, as opposed to another aptamer-based technique with a similar limit of detection that was useful for qualitative analysis only [17].…”

“…Another antibody-based assay had a dynamic range over 4•× 10 5 -10 9 VP/mL with setup duration more than 3.5 h for step-by-step adsorption of the assay components [12]. The aptasensor with similar characteristics allowed determination of 10 4 -10 6 VP/mL; the experimental setup was rather quick (>10 min) but needed a sophisticated multilayer substrate [13]. Our aptasensor had LOD 2•× 10 5 VP/mL and a dynamic range of 2•× 10 5 -2•× 10 6 VP/mL; however, the time of the assay was below 15 min, and the AgNP preparation was very simple.…”

“…Their limit of detection (LOD) is higher by 3-4 orders compared to PCR techniques, being more than 10 6 viral particles per mL (VP/mL) [7][8][9][10]. A number of extremely sensitive techniques have been developed, but they have not been optimized for routine usage because of long-lasting sophisticated sample preparation and/or usage of expensive high-technology equipment [3,[12][13][14][15]. The development of simple, rapid and sensitive techniques for virus determination is in progress [3].…”

Recent Advances in Aptamer Sensors

“…The aptamers can be functional for native conformations of target molecules on live cells, so cell surface transmembrane proteins can be considered as targets [3][4][5][6]. The 3D folded structure permits the formation of stable complexes with various targets, such as proteins, nucleic acids, and small molecules [7][8][9][10][11][12][13]. Compared to antibodies, these aptamers are characterized by numerous advantages such as a wide range of targeted molecules (inorganics, organics, cells, viruses, and bacteria, among others), facile preparation on a massive scale, low molecular weights, high temperature and pH stability, easier modification, and long-term storage stability.…”

Pre-clinically evaluated visual lateral flow platform using influenza A and B nucleoprotein as a model and its potential applications

“…Of those technologies developed for a rapid pathogen detection i.e. isothermal nucleic acid amplication, micro-uidic, three-dimensional nanostructured substrates, and mobile handheld technologies, [9][10][11][12][13] and lateral ow immunoassay, the latter technique has been used increasingly as POC and point-of-need applications, 14 especially in low-resource settings where laboratory facilities are limited. 15,16 These include the tests on biomarkers, 17 infectious agents, 18 and toxic compounds.…”