SERS‐Marker für die Anregung mit rotem Laserlicht: Glasverkapselte SAMs auf Gold/Silber‐Nanoschalen

Abstract

SAM im Glashaus: Glasverkapselte selbstorganisierte Monolagen (SAMs) auf durchstimmbaren Gold/Silber‐Nanoschalen wurden als Markierungsreagens für die oberflächenverstärkte Raman‐Streuung (SERS) eingesetzt. Dieses Konzept vereint die spektroskopischen Vorteile einer maximalen Oberflächenbedeckung und der einheitlichen molekularen Orientierung der Raman‐Markermoleküle innerhalb der vollständigen Monolage mit der hohen Stabilität einer Glashülle.

SERS Microscopy: Nanoparticle Probes and Biomedical Applications

“…[18] In contrast to the sub-monolayer coverage with Raman labels, a SAM leads to a higher sensitivity. Using gold/silver nanoshells and red laser excitation (632.8 nm), it has been shown experimentally that a complete SAM yields ca.…”

Direct Silica Encapsulation of Self‐Assembled‐Monolayer‐Based Surface‐Enhanced Raman Scattering Labels with Complete Surface Coverage of Raman Reporters by Noncovalently Bound Silane Precursors

“…[1,2] Functionalized metal colloids for use as surfaceenhanced Raman scattering (SERS) labels offer attractive advantages over conventional strategies. [12] An optional shell for protection and further biofunctionalization is favorable. [7][8][9][10][11] A SERS label consists of a metal nanoparticle for signal enhancement upon excitation of the localized surface plasmon resonance and organic molecules as the Raman reporters adsorbed onto the metal surface for spectral identification.…”

“…[3][4][5][6] A particular advantage is the extremely high spectral multiplexing capacity, that is, the ability to spectrally differentiate many labels within only a single measurement due to the small linewidth of vibrational Raman bands. Different shell types, including (bio)polymers [13,14] and silica, [12,[15][16][17][18] have been reported. A complete self-assembled monolayer (SAM) of Raman reporters provides maximum signal levels combined with reproducible Raman signatures due to the uniform orientation of the reporters within the SAM.…”

“…[12] b) A previously reported synthesis that uses Raman reporter molecules with covalently bound terminal SiO 2 precursors. SAM-based SERS labels for silica encapsulation.…”

“…SAM-based SERS labels for silica encapsulation. [12] A disadvantage of this approach is the number of synthesis steps because losses are associated with each step. [12] b) A previously reported synthesis that uses Raman reporter molecules with covalently bound terminal SiO 2 precursors.…”