SERS-Microfluidic Approach for the Quantitative Detection of miRNA Using DNAzyme-Mediated Reciprocal Signal Amplification

Ma, Lindong; Ye, Sujuan; Wang, Xingxiang; Zhang, Jihua

doi:10.1021/acssensors.1c00063

Cited by 45 publications

(18 citation statements)

References 32 publications

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“…Nucleic acid engineering can produce many cyclic products from a single target by rationally-designed nucleic acid sequences. Based on this concept, nuclease-assisted amplification methods have been adopted to fabricate miRNAs assays 94 , 95 . Briefly, DNA in the hybrid duplex chain of DNA and miRNA will hydrolyze in the presence of exonuclease or duplex-specific nuclease.…”

Section: Introductionmentioning

confidence: 99%

SERS Tags for Biomedical Detection and Bioimaging

Liu¹,

Gao²,

Xu³

et al. 2022

Theranostics

161

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Surface-enhanced Raman scattering (SERS) has emerged as a valuable technique for molecular identification. Due to the characteristics of high sensitivity, excellent signal specificity, and photobleaching resistance, SERS has been widely used in the fields of environmental monitoring, food safety, and disease diagnosis. By attaching the organic molecules to the surface of plasmonic nanoparticles, the obtained SERS tags show high-performance multiplexing capability for biosensing. The past decade has witnessed the progress of SERS tags for liquid biopsy, bioimaging, and theranostics applications. This review focuses on the advances of SERS tags in biomedical fields. We first introduce the building blocks of SERS tags, followed by the summarization of recent progress in SERS tags employed for detecting biomarkers, such as DNA, miRNA, and protein in biological fluids, as well as imaging from in vitro cell, bacteria, tissue to in vivo tumors. Further, we illustrate the appealing applications of SERS tags for delineating tumor margins and cancer diagnosis. In the end, perspectives of SERS tags projecting into the possible obstacles are deliberately proposed in future clinical translation.

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Section: Introductionmentioning

confidence: 99%

SERS Tags for Biomedical Detection and Bioimaging

Liu¹,

Gao²,

Xu³

et al. 2022

Theranostics

161

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“…MicroRNAs (miRNAs) are a sort of small noncoding and single-stranded RNA with the length of 18–25 nucleotides, which play critical roles in the regulation of gene expression at the translation level. − Many reported studies have shown that the abnormal or mutation expression of certain miRNAs is connected with the pathogenesis of some cancers, making them a valuable biomarker for early diagnosis of various cancers including lung, breast, colorectal, prostate, and so forth. − For instance, in a perspective view, miRNA-21 was reported to have overexpression in various cancer cell lines and was regarded to be closely related to the chemotherapeutic sensitivity of tumors. Thus, the development of analytical technologies that can accurately detect miRNA expression levels is very important for the early disease diagnosis and the monitoring of the therapeutic effect. ,, However, the highly efficient analysis of miRNAs is especially challenging due to their short length, facile degradation, low cellular abundance, and high sequence homology. , In the last several years, many efforts have been made to build biosensing platforms for miRNA assay, for example, fluorescence, , colorimetry, , electrochemiluminescence, − surface-enhanced Raman spectroscopy, , electrochemical , and photoelectrochemical (PEC) , methods, and so forth. Among these platforms, the PEC biosensor has attracted widespread attention due to its advantages of low background signal, excellent sensitivity and stability, especially the advantage of both optical and electrochemical methods by combining photoexcitation and electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

Jiang

et al. 2023

Anal. Chem.

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Sensitive and specific assay of microRNAs (miRNAs) is beneficial to early disease screening. Herein, we for the first time proposed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-mediated photoelectrochemical biosensors for the direct assay of miRNA-21. In this study, compared with traditional nucleic acid-based signal amplification strategies, the CRISPR/Cas13a system can greatly improve the specificity and sensitivity of target determination due to its accurate recognition and high-efficient trans-cleavage capability without complex nucleic acid sequence design. Moreover, compared with the CRISPR/Cas12a-based biosensing platform, the developed CRISPR/Cas13a-mediated biosensor can directly detect RNA targets without signal transduction from RNA to DNA, thereby avoiding signal leakage and distortion. Generally, the proposed biosensor reveals excellent analysis capability with a wider linear range from 1 fM to 5 nM and a lower detection limit of 1 fM. Additionally, it also shows satisfactory stability in the detection of human serum samples and cell lysates, manifesting that it has great application prospects in the areas of early disease diagnosis and biomedical research.

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“…In order to improve the analytical sensitivity in the quantification of miRNAs, signal amplification technologies have been introduced, such as rolling circle amplification (RCA), , exonuclease assisted amplification, , catalytic hairpin self-assembly (CHA), − hybrid chain reaction (HCR), , strand displacement reaction (SDR), , and deoxyribozyme (DNAzyme). , Multicomponent nucleic acid enzymes (MNAzyme) are derived from DNAzyme with higher selectivity and more flexible design . MNAzyme distributes the catalytic core into two partial enzymes (partzyme A and partzyme B), which are composed of a partial catalytic core, a substrate arm and a sensor arm.…”

Section: Introductionmentioning

confidence: 99%

DNA Tetrahedron-Based MNAzyme for Sensitive Detection of microRNA with Elemental Tagging

Liu

et al. 2021

ACS Appl. Mater. Interfaces

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Heterogeneous immunoassay based on magnetic separation is commonly used in inductively coupled plasma-mass spectrometry (ICP-MS)-based biomedical analysis with elemental labeling. However, the functionalized magnetic beads (MBs) often suffer from non-specific adsorption and random distribution of the functional probes. To overcome these problems, DNA tetrahedron (DT)-functionalized MBs were designed and further conjugated with substrate modified Au NPs (Sub-AuNP). Based on the prepared MB-DT-AuNP probes, an MB-DT based multicomponent nucleic acid enzyme (MNAzyme) system involving Au NPs as the elemental tags was proposed for highly sensitive quantification of miRNA-155 by ICP-MS. Target miRNA would trigger the assembly of MNAzyme, and Sub-AuNP would be cleaved from the MB-DT-AuNP probe, resulting in a cyclic amplification. Single-stranded DNA-functionalized MB (MB-ssDNA)-AuNP probes were prepared as well. Comparatively, the amount of Au NPs grafted onto MB-ssDNA-AuNP probes was higher than that grafted onto MB-DT-AuNP probes. Meanwhile, a higher signal-to-noise ratio was obtained by using MB-DT-AuNP probes over MB-ssDNA-AuNP probes in the MNAzyme system. Under the optimal experimental conditions, the limit of detection for target miRNA obtained by using MB-DT-AuNP probes was 1.15 pmol L–1, improved by 23 times over that obtained by the use of MB-ssDNA-AuNP probes. The proposed MB-DT-MNAzyme-ICP-MS method was applied to the analysis of miRNA-155 in serum samples, and recoveries of 86.7–94.6% were obtained. This method is featured with high sensitivity, good specificity, and simple operation, showing a great application potential in biomedical analysis.

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SERS-Microfluidic Approach for the Quantitative Detection of miRNA Using DNAzyme-Mediated Reciprocal Signal Amplification

Cited by 45 publications

References 32 publications

SERS Tags for Biomedical Detection and Bioimaging

SERS Tags for Biomedical Detection and Bioimaging

Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

DNA Tetrahedron-Based MNAzyme for Sensitive Detection of microRNA with Elemental Tagging

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