SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Wu, Yixuan; Dang, Hajun; Park, Sung‐Gyu; Chen, Lingxin; Choo, Jaebum

doi:10.1016/j.bios.2021.113736

Cited by 40 publications

(32 citation statements)

References 31 publications

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“…SERS is an effective technique for the sensitive detection of biomolecules, and various SERS-based immunoassays have thus been developed for the detection of infectious viruses ( Saviñon-Flores et al, 2021 ). Following the emergence of the COVID-19 pandemic, several SERS-based methods for SARS-CoV-2 have been applied ( Zhang et al, 2021 ; Wu et al, 2022 ; Yang et al, 2021 ; Yu et al, 2021 ; Srivastav et al, 2021 ; Sanchez et al, 2021 ; Payne et al, 2021 ; Liu et al, 2021 ; Li et al, 2021 ; Huang et al, 2021 ; Gao et al, 2021 ; Chen et al, 2021 ; Chen et al, 2021 , 2021 ; Huang et al, 2021 , 2021 ; Pramanik et al, 2021 ; Zavyalova et al, 2021 ). However, the current magnetically assisted SERS immunoassay is the only assay developed so far that facilitates the on-site diagnosis of the samples of COVID-19 patients.…”

Section: Discussionmentioning

confidence: 99%

“…However, recent advancements in SERS have led to the development of sophisticated sensing methods that facilitate precise analysis and generation of high-quality SERS-active nanostructures ( Hwang et al, 2019 ; Shin et al, 2018 ). In particular for the combat with COVID-19, SERS technology has not only exhibited less time-cost advantages than PCR for detection SARS-CoV-2 and antibodies, but also exhibited more advantages such as identifying infectiousness of SARS-CoV-2 ( Peng et al, 2021a , Peng et al, 2021b ; Zhang et al, 2021 ; Wu et al, 2022 ; Yang et al, 2021 ; Yu et al, 2021 ; Srivastav et al, 2021 ; Sanchez et al, 2021 ; Payne et al, 2021 ; Liu et al, 2021 ; Li et al, 2021 ; Huang et al, 2021 ; Gao et al, 2021 ; Chen et al, 2021 ; Chen et al, 2021 , 2021 ; Huang et al, 2021 , 2021 ; Pramanik et al, 2021 ; Zavyalova et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cha

Kim

Joung

et al. 2022

Biosensors and Bioelectronics

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Cha

Kim

Joung

et al. 2022

Biosensors and Bioelectronics

Self Cite

View full text Add to dashboard Cite

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“…Based on the aggregation of AuNPs, the presented biosensor achieved a LOD of femtomole in all modes, which is 160 fM in absorbance mode, 259 fM in fluorescence mode, and 395 fM in SERS mode. Wu and co-workers [ 92 ] suggested a SERS-PCR strategy for the detection of SARS-CoV-2 genes (E and RdRP genes) using AuNP-internalized Au nanodimple substrates (AuNDS). According to the obtained results from RT-PCR, SERS-PCR, and AuNDS-based SERS-PCR, the detectable concentrations of genes were found to be 3.36 × 10 12 , 3.28 × 10 9 , and 2.56 × 10 7 copies μL −1 , respectively.…”

Section: Sers-based Sensing Methodsmentioning

confidence: 99%

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

2022

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The outbreak rate of human coronaviruses (CoVs) especially highly pathogenic CoVs is increasing alarmingly. Early detection of these viruses allows treatment interventions to be provided more quickly to people at higher risk, as well as helping to identify asymptomatic carriers and isolate them as quickly as possible, thus preventing the disease transmission chain. The current diagnostic methods such as RT-PCR are not ideal due to high cost, low accuracy, low speed, and probability of false results. Therefore, a reliable and accurate method for the detection of CoVs in biofluids can become a front-line tool in order to deal with the spread of these deadly viruses. Currently, the nanomaterial-based sensing devices for detection of human coronaviruses from laboratory diagnosis to point-of-care (PoC) diagnosis are progressing rapidly. Gold nanoparticles (AuNPs) have revolutionized the field of biosensors because of the outstanding optical and electrochemical properties. In this review paper, a detailed overview of AuNP-based biosensing strategies with the varied transducers (electrochemical, optical, etc.) and also different biomarkers (protein antigens and nucleic acids) was presented for the detection of human coronaviruses including SARS-CoV-2, SARS-CoV-1, and MERS-CoV and lowly pathogenic CoVs. The present review highlights the newest trends in the SARS-CoV-2 nanobiosensors from the beginning of the COVID-19 epidemic until 2022. We hope that the presented examples in this review paper convince readers that AuNPs are a suitable platform for the designing of biosensors. Graphical abstract

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“… 10 After several weeks of infection, IgG reactivity reaches >98%, but the duration of this antibody response is still unclear. Currently, serological tests, 11 , 12 , 13 lateral‐flow immunoassay, 14 chemiluminescence immunoassay, 13 surface‐enhanced Raman scattering (SERS)‐PCR, 15 , 16 , 17 and fluorescence immunoassay 18 are important tools for monitoring immunity after a SARS‐CoV‐2 infection or vaccination. These methods, however, suffer from low sensitivity and specificity and are unable to detect low concentrations of antibodies.…”

Section: Introductionmentioning

confidence: 99%

N protein‐based ultrasensitive SARS‐CoV‐2 antibody detection in seconds via 3D nanoprinted, microarchitected array electrodes

Ali

Zhang

et al. 2022

Journal of Medical Virology

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Rapid detection of antibodies to SARS-CoV-2 is critical for COVID-19 diagnostics, epidemiological research, and studies related to vaccine evaluation. It is known that the nucleocapsid (N) is the most abundant protein of SARS-CoV-2 and can serve as an excellent biomarker due to its strong immunogenicity. This paper reports a rapid and ultrasensitive 3D biosensor for quantification of COVID-19 antibodies in seconds via electrochemical transduction. This sensor consists of an array of threedimensional micro-length-scale electrode architecture that is fabricated by aerosol jet 3D printing, which is an additive manufacturing technique. The micropillar array is coated with N proteins via an intermediate layer of nano-graphene and is integrated into a microfluidic channel to complete an electrochemical cell that uses antibodyantigen interaction to detect the antibodies to the N protein. Due to the structural innovation in the electrode geometry, the sensing is achieved in seconds, and the sensor shows an excellent limit of detection of 13 fm and an optimal detection range of 100 fm to 1 nM. Furthermore, the sensor can be regenerated at least 10 times, which reduces the cost per test. This work provides a powerful platform for rapid screening of antibodies to SARS-CoV-2 after infection or vaccination.

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SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

Cited by 40 publications

References 31 publications

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

N protein‐based ultrasensitive SARS‐CoV‐2 antibody detection in seconds via 3D nanoprinted, microarchitected array electrodes

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