SERS study of methylated and nonmethylated ribonucleosides and the effect of aggregating agents

Hobro, Alison J.; Abdali, Salim; Blanch, Ewan W.

doi:10.1002/jrs.3029

Cited by 15 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectral peaks in Fig. 6c are also somewhat similar to the spectra of adenosine and guanosine 24,32 , albeit with a peak shift towards the red of 20 cm À 1 .…”

Section: Nature Communications | Doi: 101038/ncomms6144mentioning

confidence: 53%

“…This emphasizes the importance of spatial resolution and targeted nanoparticle fabrication. In typical SERS measurements of a purified analyte, aggregation of nanoparticles is often intentionally induced since it changes the amount of detectable signal 24 . In the photoreduction process, the detection of SERS from fabricated regions likely occurs due to the growth of small particles, trapping biological molecules between particles, at hotspots in the enhanced field, creating ideal conditions for measurement.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

Self Cite

View full text Add to dashboard Cite

Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

show abstract

“…The spectral peaks in Fig. 6c are also somewhat similar to the spectra of adenosine and guanosine 24,32 , albeit with a peak shift towards the red of 20 cm À 1 .…”

Section: Nature Communications | Doi: 101038/ncomms6144mentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hobro, Abdali, and Blanch reported a SERS study of methylated and nonmethylated ribonucleosides and the effect of aggregating agents. Their work shows that the selection of aggregating agent plays a crucial role for SERS analysis of these nucleosides with K 2 SO 4 generating immediate enhancement, whereas NaCl gives immediate enhancement only for the pyrimidine nucleosides . Jurasekova et al .…”

Section: Sersmentioning

confidence: 99%

Recent advances in linear and nonlinear Raman spectroscopy. Part VII

Nafie

2013

J Raman Spectroscopy

View full text Add to dashboard Cite

show abstract

“…However, label-free methods do have the distinct advantage that they are intrinsically sensitive to chemical modifications of DNA, since chemical modification of nucleobases causes characteristic changes in their Raman spectra. [6][7][8][9][10][11][12][13][14] For example, it has been shown that substitution of even a single base in 25-mer oligodeoxynucleotides (ODNs) can be detected using SERS difference spectroscopy. 6 This sensitivity to changes in chemical structure means that SERS may be also used to detect post-transcription modifications of nucleobases, which are both important from a physiological/clinical viewpoint and difficult to detect through hybridisation assays.…”

Section: Introductionmentioning

confidence: 99%

Quantitative surface-enhanced Raman spectroscopy of single bases in oligodeoxynucleotides

Dick

Bell

2017

Faraday Discuss.

View full text Add to dashboard Cite

To address the question of whether the SERS signals of ss-DNA are simply combinations of the signals from the individual bases that comprise the sequence, SERS spectra of unmodified ss-DNA sequences were obtained using a hydroxylamine-reduced Ag colloid aggregated with MgSO. Initially, synthetic oligodeoxynucleotides with systematic structural variations were used to investigate the effect of adding single nucleobases to the 3' terminus of 10-mer and 20-mer sequences. It was found that the resulting SERS difference spectra could be used to identify the added nucleobases since they closely matched reference spectra of the same nucleobase. Investigation of the variation in intensity of an adenine probe which was moved along a test sequence showed there was a small end effect where nucleobases near the 3' terminus gave slightly larger signals but the effect was minor (30%). More significantly, in a sample set comprising 25-mer sequences where A, T or G nucleobases were substituted either near the centres of the sequences or the 5' or 3' ends, the SERS difference spectra only matched the expected form in approximately half the cases tested. This variation appeared to be due to changes in secondary structure induced by altering the sequences since uncoiling the sequences in a thermal pre-treatment step gave difference spectra which in all cases matched the expected form. Multivariate analysis of the set of substitution data showed that 99% of the variance could be accounted for in a model with just three factors whose loadings matched the spectra of the A, T, and G nucleobases and which contained no positional information. This suggests that aside from the differences in secondary structure which can be eliminated by thermal pre-treatment, the SERS spectra of the 25-mers studied here are simply the sum of their component parts. Although this means that SERS provides very little information on the primary sequence it should be excellent for the detection of post-transcription modifications to DNA which can occur at multiple positions along a given sequence.

show abstract

SERS study of methylated and nonmethylated ribonucleosides and the effect of aggregating agents

Cited by 15 publications

References 30 publications

Laser-targeted photofabrication of gold nanoparticles inside cells

Laser-targeted photofabrication of gold nanoparticles inside cells

Recent advances in linear and nonlinear Raman spectroscopy. Part VII

Quantitative surface-enhanced Raman spectroscopy of single bases in oligodeoxynucleotides

Contact Info

Product

Resources

About