Sertoli cell‐derived exosomal MicroRNA‐486‐5p regulates differentiation of spermatogonial stem cell through PTEN in mice

Li, Quan; Li, Hanhao; Liang, Jinlian; Mei, Jiaxin; Cao, Zhen; Zhang, Lei; Luo, Jiao; Tang, Yan; Huang, Rufei; Xia, Huan; Zhang, Qihao; Xiang, Qi; Yang, Yan; Huang, Yadong

doi:10.1111/jcmm.16347

Cited by 29 publications

(17 citation statements)

References 32 publications

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“…The observations of Li et al 2021 about Sertoli cell miR-486-5p containing exosomes adds to the evolving school of thought on how undifferentiated spermatogonia enter meiosis ( 54 ). Spermatogonial differentiation and meiotic entry is established to be highly dependent on retinoic acid (RA) signaling ( 55 , 56 ).…”

Section: Endocrine and Paracrine Signalsmentioning

confidence: 93%

“…Paracrine signaling from Sertoli to germ cells by exosomes containing microRNA would putatively be to silence genes. Indeed, a recent report by Li et al 2021, revealed that Sertoli exosomes contain the microRNA miR-486-5p ( 54 ). The authors used a culture system of adult Sertoli cells and P6 germ cells enriched for spermatogonial stem cells.…”

Section: Endocrine and Paracrine Signalsmentioning

confidence: 99%

“…and Sycp3 were indirectly up-regulated in spermatogonial stem cells by the decrease in repressive PTEN. Ultimately this exosome exchange would seem to be part of the differentiation signal from Sertoli cells to spermatogonia ( 54 ).…”

Section: Endocrine and Paracrine Signalsmentioning

confidence: 99%

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Updates in Sertoli Cell-Mediated Signaling During Spermatogenesis and Advances in Restoring Sertoli Cell Function

Ruthig

Lamb

2022

Front. Endocrinol.

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Since their initial description by Enrico Sertoli in 1865, Sertoli cells have continued to enchant testis biologists. Testis size and germ cell carrying capacity are intimately tied to Sertoli cell number and function. One critical Sertoli cell function is signaling from Sertoli cells to germ cells as part of regulation of the spermatogenic cycle. Sertoli cell signals can be endocrine or paracrine in nature. Here we review recent advances in understanding the interplay of Sertoli cell endocrine and paracrine signals that regulate germ cell state. Although these findings have long-term implications for treating male infertility, recent breakthroughs in Sertoli cell transplantation have more immediate implications. We summarize the surge of advances in Sertoli cell ablation and transplantation, both of which are wedded to a growing understanding of the unique Sertoli cell niche in the transitional zone of the testis.

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Section: Endocrine and Paracrine Signalsmentioning

confidence: 93%

Section: Endocrine and Paracrine Signalsmentioning

confidence: 99%

See 1 more Smart Citation

Updates in Sertoli Cell-Mediated Signaling During Spermatogenesis and Advances in Restoring Sertoli Cell Function

Ruthig

Lamb

2022

Front. Endocrinol.

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“…MiR-34c promotes SSC differentiation by inhibiting the function of the NANOS2 gene, leading to the up-regulation of meiotic-related proteins, STRA8, in mice ( 56 ). Similarly, miR-486-5p secreted by Sertoli cells stimulates differentiation of SSCs in mice by up-regulating the expression of STRA8 and SYCP3 ( 57 ). Further, impaired spermatogenesis is observed in mice carrying a deficiency in miR-17-92 or a gene deletion of miR-17-92 ( 58 , 59 ).…”

Section: Regulation Of Sscsmentioning

confidence: 99%

Roles of Spermatogonial Stem Cells in Spermatogenesis and Fertility Restoration

et al. 2022

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Spermatogonial stem cells (SSCs) are a group of adult stem cells in the testis that serve as the foundation of continuous spermatogenesis and male fertility. SSCs are capable of self-renewal to maintain the stability of the stem cell pool and differentiation to produce mature spermatozoa. Dysfunction of SSCs leads to male infertility. Therefore, dissection of the regulatory network of SSCs is of great significance in understanding the fundamental molecular mechanisms of spermatogonial stem cell function in spermatogenesis and the pathogenesis of male infertility. Furthermore, a better understanding of SSC biology will allow us to culture and differentiate SSCs in vitro, which may provide novel stem cell-based therapy for assisted reproduction. This review summarizes the latest research progress on the regulation of SSCs, and the potential application of SSCs for fertility restoration through in vivo and in vitro spermatogenesis. We anticipate that the knowledge gained will advance the application of SSCs to improve male fertility. Furthermore, in vitro spermatogenesis from SSCs sets the stage for the production of SSCs from induced pluripotent stem cells (iPSCs) and subsequent spermatogenesis.

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“…Another non-coding RNAs, miRNAs, are members of endogenous small RNA with the length of about 18–24 nucleotides, involved in the regulation of mRNA expression. By binding to the 3′-untranslated region, miRNAs block the translation or induce the degradation of the target mRNA, thus modulating cell differentiation, growth, proliferation and apoptosis ( Li et al, 2021 ; Mohammadi et al, 2021 ; Xiang et al, 2021 ). miRNAs have been reported as diagnostic markers and therapeutic targets for the control of diseases ( Broermann et al, 2020 ; Tombolan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

Liu

et al. 2022

Front. Cell Dev. Biol.

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The larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the liver in response to E. multilocularis infection will help us to understand its pathogenesis. Using liver perfusion, different cell populations including the hepatic cells, hepatic stellate cells and Kupffer cells were isolated from mice interperitoneally inoculated with protoscoleces. Their transcriptional profiles including lncRNAs, miRNAs and mRNAs were done by RNA-seq. Among these cell populations, the most differentially-expressed (DE) mRNA, lncRNAs and miRNAs were annotated and may involve in the pathological processes, mainly including metabolic disorders, immune responses and liver fibrosis. Following the integrative analysis of 38 differentially-expressed DEmiRNAs and 8 DElncRNAs, the lncRNA-mRNA-miRNA networks were constructed, including F63-miR-223-3p-Fbxw7/ZFP36/map1b, F63-miR-27-5p-Tdrd6/Dip2c/Wdfy4 and IFNgAS1-IFN-γ. These results unveil the presence of several potential lncRNA-mRNA-miRNA axes during E. multilocularis infection, and further exploring of these axes may contribute to better understanding of the pathogenic mechanisms.

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Sertoli cell‐derived exosomal MicroRNA‐486‐5p regulates differentiation of spermatogonial stem cell through PTEN in mice

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 29 publications

References 32 publications

Updates in Sertoli Cell-Mediated Signaling During Spermatogenesis and Advances in Restoring Sertoli Cell Function

Updates in Sertoli Cell-Mediated Signaling During Spermatogenesis and Advances in Restoring Sertoli Cell Function

Roles of Spermatogonial Stem Cells in Spermatogenesis and Fertility Restoration

Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis

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