Sertoli cell origin of testicular androgen-binding protein (ABP)

Hagenäs, Lars; Ritzén, E. M.; Plöen, L.; Hansson, V.; French, Frank S.; Nayfeh, Shihadeh N.

doi:10.1016/0303-7207(75)90021-0

Cited by 122 publications

(35 citation statements)

References 18 publications

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“…This approach has been applied in blood plasma of several animal species, including man [5] and also for analyzing the testicle, epididymis, and occasionally seminal plasma. Hagenas et al [13] localized in rat seminal fluid a protein probably of testicular origin capable of binding androgens (ABP). Later the same group 1141 reported that the ABP was originated in the Sertoli cells.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Characterization of a New Androgen-Binding Protein in Human Seminal Plasma

Barahona

Banuelos

Solís

et al. 1980

Archives of Andrology

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In order to obtain comparative analysis between the steroid binding proteins from blood serum and seminal plasma, fresh samples were incubated and assayed in parallel with the following tritiated steroids: dihydrotestosterone, testosterone, cortisol, progesterone and estradiol. Thereafter, and using techniques such as (1) polyacrilamide disk gel electrophoresis with elution of proteins from the gel slices, (2) ammonium sulphate precipitation, (3) enzymatic hydrolysis, and (4) gel filtration on Sephadex G-100, there was a resemblance in the electrophoretic patterns confirming the presence in both fluids of proteins like TeBG, CBG, and Albumin, previously described in blood serum. In seminal fluid, but not in blood serum, another small protein with androgen binding capacity was found and its preliminary characterization demonstrated: disappearance with enzymatic treatment, low molecular weight, negative charge, and precipitation range with ammonium sulphate from 30 to 35%. Its elution profile on Sephadex G-100 was contaminated with albumin.

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Section: Introductionmentioning

confidence: 99%

Preliminary Characterization of a New Androgen-Binding Protein in Human Seminal Plasma

Barahona

Banuelos

Solís

et al. 1980

Archives of Andrology

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“…During spermatogenesis in the testis, Sertoli cells keep contact with germ cells after the preleptotene stage, and secrete androgen-binding protein (ABP). However, FSH stimulates Sertoli cells to produce cAMP and secretes ABP into the seminiferous tubules, and eventually ABP binding testosterone maintains spermatogenesis (Hagenas et al 1975). Sertoli cells also contribute to germ cell development by the secretion of transferrin, an iron transporter (Skinner et al 1984), ceruloplasmine-like protein (Skinner & Griswold 1983) and plasminogen activator (Lacroix et al 1977), together with a supply of pyruvic acid and lactic acid to germ cells as an energy source (Jutte et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of headless sperms in adolescent rat urine

Shimomura¹,

Sakurai²,

Shimada³

et al. 2008

Lab Anim

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SummaryIncreased incidence of headless sperms (HS) was spontaneously observed in the urine of adolescent naïve male SPF/VAF Crl:CD(SD) rats. To clarify the factors contributing to this event, the HS incidence in urine and the epididymis was periodically examined in conjunction with measurements of testis and epididymis weights, motility and morphology of sperms and testosterone, transferrin or follicle-stimulating hormone (FSH) concentrations in serum and/or the testis. The urinary HS incidence was 61%, 69%, 44%, 30%, 14%, 9% and 7% in 100 sperms counted at ages 8, 9, 10, 11, 12, 13 and 14 weeks, respectively; namely, HS peaked at 9 weeks, gradually decreased from 10 weeks and became almost a plateau from 12 weeks onwards. The epididymal HS incidence, which was lower than that in urine, peaked at 8 weeks, decreased from 10 weeks and became almost zero from 12 weeks. By scanning electron microscopy of HS in the epididymis, a narrow gap between the sperm head and neck was clearly seen along with the posterior ring. Concentrations of testicular testosterone and transferrin, a marker for Sertoli cell maturation, reached mature animal levels at 12 weeks. In contrast, no change in serum FSH concentration was seen throughout the study period. These results demonstrate that a marked increase in urinary HS incidence in naïve rats at ages 8-11 weeks would be a physiological phenomenon seen in connection with the process of Sertoli cell maturation.

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“…In vivo, il n'y a pas de raison pour que la SBPh, produite majoritairement par le foie et confin6e au compartiment plasmatique [30] entre en contact avec les cellules de la lign6e germinale situ6es au delh de la barri6re sang-testicule, /~ l'inverse de I'ABP produite par les cellules de Sertoli [10,19,24,39]. N6anmoins on sait que ces prot6ines ont un g6ne commun [24] et que du point de vue de leur s6quence en AA la SBPh et l'ABPr ont une homologie de 68% sur les 373 r6sidus qu'elles comportent l'une et l'autre [23,24].…”

Section: Figure 11-13 : L N T E R N a L I S A T I O N Du Complexe S Bunclassified

Germinal cell lineage possess the capacity of sex steroid-binding protein (hSBP) internalization: Transmission electron microscopy (TEM) autohistoradiographic study

Bedjou¹,

Gérard

Felden

et al. 1995

Androl.

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RESUMEI1 a dt~ rdcemment ddmontrd que les cellules spermatogdndtiques de rat sont capables de lier et d'internaliser spdcifiquement l'androgen-binding protein de rat (ABPr). De m~me les cellules spermatog~ndtiques du macaque, sont capables de lier et d'internaliser spdcifiquement la sex steroid-binding protein humaine (SBPh).Cette dtude consiste ~ tester si des interactions entre les cellules spermatogdndtiques et les prot~ines de liaison des st~roides existent dgalement chez l'homme. Les cellules germinales proviennent d'une part des biopsies testiculaires de patients hypofertiles et d'autre part de pulpectomies indiqu~es pour cancer prostatique.Les observations en MET rdv~lent la prdsence de deux types de structures relids ~ l'endocytose dans les cellules spermatogdndtiques. Premi~rement, les puits et vdsicules recouverts de 96 _+ 10 nm de diam~tre, associds ~ la membrane plasmique. Deuxi~mement, les endosomes prdcoces (vdsicules non recouvertes) de 225 -+ 60 nm de diam~tre localisds ~ la pdriph~rie du cytoplasme ainsi que les endosomes tardifs, souvent organisds en corps multiv~siculaires (CMV) dans le cytoplasme pdrinucldaire. Ces deux types de structures de l'appareil endocytaire sont prdsents ~ tous les stades de maturation des cellules germinales. Les vdsicules non recouvertes sont toujours plus nombreuses que les vdsicules recouvertes quel que soit le stade.Les cellules germinales isoldes et in situ maintenues dans l'dpithdlium sdminif/~re ont ~td exposdes au milieu de culture contenant le complexe SBPh purifi~e (~ partir de sdrum de femme enceinte) -A6-testotdrone tritide (80 000 cpm/ml, 30 ng) photomarqude. L'~tude des interactions SBPh/ cellules germinales est fond~e sur les 528 analyses qualitatives et quantitatives du marquage r~v~l~ par autohistoradiographie en MET.

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Sertoli cell origin of testicular androgen-binding protein (ABP)

Cited by 122 publications

References 18 publications

Preliminary Characterization of a New Androgen-Binding Protein in Human Seminal Plasma

Preliminary Characterization of a New Androgen-Binding Protein in Human Seminal Plasma

Occurrence of headless sperms in adolescent rat urine

Germinal cell lineage possess the capacity of sex steroid-binding protein (hSBP) internalization: Transmission electron microscopy (TEM) autohistoradiographic study

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