Sertoli cell ultrostructure. I. A comparative study in immature, pubescent, adult and cryptorchid pigs

Chevalier, Michelle

doi:10.1051/rnd:19780703

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…The Sertoli-Sertoli cell junctional specializations appeared to maintain their structural integrity even among cells in advanced stages of degeneration. This observation is similar to that made of cryptorchid pigs [3], and it emphasizes the remarkable resistance of these junctions to deleterious influences [ 131.…”

Section: Discussionsupporting

confidence: 86%

Ultrastructure of Sertoli Cells in Cryptorchid Goats

Singh

Ezeasor

1989

Archives of Andrology

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Sertoli cells of intra-abdominal testes from 25 naturally occurring, unilaterally cryptorchid, West African pygmy goats between the ages of 1-30 months were morphologically examined by light and transmission electron microscopy. Ultrastructurally, Sertoli cells in the intra-abdominal testes from 1-month-old goats were columnar and contained ovoid nuclei. The cytoplasm depicted clusters of rough endoplasmic reticulum cisternae (RER). In 3- to 4-month-old animals nucleolonemas of the Sertoli cells were associated with vesicles; the cytoplasm contained RER and areas of whorls of smooth endoplasmic reticulum. An abundance of lipid droplets was accompanied by lipofuscin granules in the cytoplasm. In 6- to 8-month-old goats RER cisternae presented circular or irregular profiles in the cells with an augmentation of cytoplasmic lipid droplets and lipofuscin granules. In 12- to 15-month-old animals intercellular spaces in the seminiferous epithelium exhibited focal expansions. Nuclear profiles of the Sertoli cells were irregular. In 24- to 30-month-old animals the Sertoli cells were in an advanced stage of degeneration.

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Section: Discussionsupporting

confidence: 86%

Ultrastructure of Sertoli Cells in Cryptorchid Goats

Singh

Ezeasor

1989

Archives of Andrology

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“…In addition to these easily recognizable Sertoli cells, another group of cells was immunoreactive for GATA-4. These type B Sertoli cells had small round nuclei containing distinct nucleoli, substantially less cytoplasm, and were always located very near the basement membrane of the seminiferous epithelium as described by Chevalier [6]. Type B Sertoli cells are not easily recognizable as Sertoli cells using light microscopy, and it is probable that their nuclei are not classified as Sertoli cell nuclei using classical evaluation procedures.…”

Section: Methodsmentioning

confidence: 93%

“…In the sexually mature boar testis, two types of Sertoli cells can be identified at the electron microscope level. Type A Sertoli cells have fine structural characteristics similar to Sertoli cells observed in other species; i.e., a light staining nucleus, and contain numerous filaments and ribosomes, abundant smooth endoplasmic reticulum (SER), and typical junctions with adjacent Sertoli cells and germ cells [6]. These cells extend from the basement membrane to the lumen of the tubule and are routinely classified as Sertoli cells even at the light microscope level.…”

Section: Introductionmentioning

confidence: 97%

“…Type B Sertoli cells are less numerous and are found only in close apposition to the basement membrane of the seminiferous epithelium. They are small cells with dark nuclei and limited cytoplasm containing SER and numerous filaments [6]. At the light microscope level, type B Sertoli cells are difficult to identify as they are similar in appearance to adjacent differentiating spermatogonia.…”

Section: Introductionmentioning

confidence: 99%

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Specific Staining of Sertoli Cell Nuclei and Evaluation of Sertoli Cell Number and Proliferative Activity in Meishan and White Composite Boars During the Neonatal Period1

McCoard

Lunstra

Wise

et al. 2001

Biology of Reproduction

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The positive relationship between Sertoli cell number and testicular size emphasizes the importance of determining factors involved in the regulation of the Sertoli cell population. Based on data from other species and indirect evidence in the boar, it is generally accepted that porcine Sertoli cells proliferate rapidly throughout the early postnatal period. However, direct evaluation of Sertoli cell number and the proliferative activity of Sertoli cells during the early postnatal period in boars have not been reported. Stereological enumeration of Sertoli cells is a labor-intensive process and would be greatly facilitated by a marker for these cells especially in the sexually mature male. Thus, the first objective of this study was to determine if expression of the transcription factor GATA-4 is an effective marker for fetal, postnatal, and adult Sertoli cells to facilitate enumeration procedures. The second objective was to evaluate the proliferative activity and growth of the Sertoli cell population in neonatal White Composite and Meishan boars, known to differ in mature testis size and Sertoli cell number, to determine the importance of this developmental period for the adult Sertoli cell population. GATA-4 was abundantly expressed by Sertoli cells throughout fetal and prepubertal stages of development and specifically stained both type A and B Sertoli cell nuclei in the sexually mature boar. Immunoreactivity was never observed in the germ cells regardless of their stage of development, illustrating that GATA-4 is a useful marker for both developing and adult Sertoli cells in the boar. Testicular size did not differ between breeds on Day 1 postpartum, but by 14 days postpartum White Composite boars had significantly larger testes compared to Meishan boars (P: < 0.001). Similarly, Sertoli cell number did not differ between breeds at 1 day postpartum; however, at 14 days postpartum White Composite boars had a significantly larger Sertoli cell population compared to Meishan boars (P: < 0.05). Surprisingly, despite having more Sertoli cells than Meishan boars at 14 days postpartum, the proportion of actively proliferating Sertoli cells in the White Composite boars was almost 50% less than the Meishan boars. This result illustrates that rapid rates of Sertoli cell proliferation probably occurred prior to 14 days postpartum in the White Composite boars. Collectively, these results illustrate that the relationship between testicular size and Sertoli cell number is manifested very early in the postnatal period for these two breeds. The substantial difference in the size of the Sertoli cell population and their proliferative activity between Meishan and White Composite boars during the early postnatal period emphasizes the importance of this early period for the establishment of the Sertoli cell population and subsequent adult testicular size.

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“…In a boar, CHEVA-L I E R (1979) encountered light and dark supporting cells in a mature testis, two months following the removal of the hypophysis in this animal. According to him it was caused by a different reactivity to the lack of gonadotropic hormones, of two types of cells which appear in a normal testis of those species (CHEVALIER, 1978). The A cells, with a more differentiated structure do not alter following the removal of the hypophysis, while B cells, normally con-served: the light and the dark ones.…”

Section: Resultsmentioning

confidence: 99%

Ultrastructure of the Bull Seminiferous Tubule Supporting Cells with Special Consideration of the Cell Junctions

Bielańska-Osuchowska

Sysa

1981

Anat Histol Embryol

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This study represents an e1ect;onmicroscopic examination of seminiferous tubule epithclium of the bull, with special attention to the cell types and junctional complexes betwcen cells whim contribute to the blood-testis barrier. In general seminiferous epithelium of the bull resembles that of other animals with some alterations in supportive cell types and organization of junctional complexes between epithelial cells. These differences are illustrated a n d discussed in regard to their possible functional significance.

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Sertoli cell ultrostructure. I. A comparative study in immature, pubescent, adult and cryptorchid pigs

Abstract: Summary. Sertoli Introduction.

Cited by 10 publications

References 18 publications

Ultrastructure of Sertoli Cells in Cryptorchid Goats

Ultrastructure of Sertoli Cells in Cryptorchid Goats

Specific Staining of Sertoli Cell Nuclei and Evaluation of Sertoli Cell Number and Proliferative Activity in Meishan and White Composite Boars During the Neonatal Period1

Ultrastructure of the Bull Seminiferous Tubule Supporting Cells with Special Consideration of the Cell Junctions

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