Serum Activity of Proteolytic Enzyme Trypsin in Rats under Conditions of Water and Food Deprivation

Kuzmina, I. V.; Ovchinnikova, N. V.; Tolpygo, S. M.

doi:10.1007/s10517-023-05910-2

Bull Exp Biol Med

2023

DOI: 10.1007/s10517-023-05910-2

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Serum Activity of Proteolytic Enzyme Trypsin in Rats under Conditions of Water and Food Deprivation

I. V. Kuzmina,

N. V. Ovchinnikova,

S. M. Tolpygo

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2024

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Cited by 2 publications

References 13 publications

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Biochemical indices of blood protein metabolism as criteria of broiler nutrition adequacy

Kuzmina,

Ovchinnikova

2024

Sib. vestn. s-h. nauki

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The experiments were conducted on the broiler chickens of the cross "Smena-9" in the vivarium of the Selective Genetic Centre "Zagorskoe Experimental Breeding Farm" in Sergiev Posad. The results of the blood plasma biochemical tests showed wavelike changes in the trypsin activity at all ages stu- died. The increased concentration of total protein at 7 days of age in all groups could have been induced by switching from endogenous feed when receiving exogenous feed containing more protein than the yolk sac. The age dynamics of the alkaline phosphatase activity had sharp fluctuations throughout the whole rearing period. At 1–7 days of age, there was an increase in the phosphatase activity with a subsequent decrease until 35 days of age relative to the control in all groups. The peak of high activity of this enzyme occurs on the 7th day – 31030 units/l in the control group and on the 14th day in the experimental group 2. Thereafter, there is a gradual decrease in the activity of this enzyme. A sharp increase in the level of alkaline phosphatase in the blood of broiler chickens at 7–21 days of age indicates intensive growth and formation of the organism, but a sharp surge in activity at 7 days of age could also be caused by the transition to a new diet, which in itself is a stress factor. A decrease in the activity of this enzyme at 28–35 days of age indicates a slowdown in the growth process. Consequently, the purpose of this study was to investigate the biochemical composition of blood in different periods of post-embryogenesis of broiler chickens of the cross "Smena-9" at changing the level of protein in the diet, which in this case remains a relevant and informative source of information on the effect of changes in the ingredient composition of feed on the bird organism as a whole.

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Biochemical indices of blood protein metabolism as criteria of broiler nutrition adequacy

Kuzmina,

Ovchinnikova

2024

Sib. vestn. s-h. nauki

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A New Method for Determining the Taste and Nutritional Properties of White Lupine in Broiler Chicken Feed

Vertiprahov,

Sergeenkova,

Bezzubenko

et al. 2024

Bulletin of KSAU

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The purpose of the study is to develop a new method for determining the taste and nutritional properties of protein supplements for broiler chickens. The experiments were performed on 20–42-day-old broiler chickens of the ROSS308 cross with a duodenal fistula. Duodenal contents were collected 60 and 120 minutes after feeding the birds to determine the taste (complex reflex phase of digestion regulation) and nutritional (neurochemical phase) properties of the protein supplement. 4 groups of chickens were formed (5 heads each): 1st control – received complete feed; 2nd experimental – grain mixture consisting of 25.0 % white lupine and 74.0 % wheat, 1.0 % premix; 3rd experimental, respectively, the ratio is 50.0/49.0 %; 4th experimental – 75.0/24.0 %, respectively. The results showed that trypsin activity 60 minutes after feeding increased by 37.6 % (p < 0.05) in the group of chickens receiving a feed mixture consisting of 25.0 % white lupine and 74.0 % wheat, compared to complete feed. The lowest digestibility was a grain mixture consisting of 75.0 % white lupine and 24.0 % wheat; trypsin activity in chyme decreased by 26.4 % (p < 0.05) compared to complete feed. At the same time, in the blood serum of broilers of the experimental group (25.0 % white lupine/74.0 % wheat), trypsin activity decreased compared to the control group by 21.5 %, which indicates a decrease in metabolic processes. Thus, the method of studying the activity of trypsin in duodenal contents in different phases of the regulation of digestion allows us to determine an effective protein supplement.

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Serum Activity of Proteolytic Enzyme Trypsin in Rats under Conditions of Water and Food Deprivation

Cited by 2 publications

References 13 publications

Biochemical indices of blood protein metabolism as criteria of broiler nutrition adequacy

Biochemical indices of blood protein metabolism as criteria of broiler nutrition adequacy

A New Method for Determining the Taste and Nutritional Properties of White Lupine in Broiler Chicken Feed

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