Serum Adipocytokines Levels and Their Association with Insulin Sensitivity in Morbidly Obese Individuals Undergoing Bariatric Surgery

Sachan, Astha; Singh, Archna; Shukla, Sagrika; Aggarwal, Sandeep; Mir, Ishfaq A.; Yadav, Rakhee

doi:10.7570/jomes20090

Cited by 8 publications

(11 citation statements)

References 37 publications

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“…The control group (n=30) included age matched individuals (18–60 years’ age) with BMI within normal range (<25 kg/m2), undergoing laparoscopic cholecystectomy, hernia repair etc. similar to our previous cross-sectional study [ 10 ]. Subjects were excluded if they had concomitant known acute or chronic disorder of the immune system, like auto-immune disorders.…”

Section: Methodssupporting

confidence: 89%

“…Plasma/serum was separated after centrifuging at 3000 rpm for 15 min. Plasma was used for measuring glucose and Insulin, while serum was transferred and stored in sterile Eppendorf tubes at −80 °C for the subsequent cytokine analysis [ 10 ]. Sample collection and processing was done in a similar manner when procuring venous blood in obese group of individuals undergoing bariatric surgery, at two more time points: at time of discharge from hospital, and at 6-month follow up visit.…”

Section: Methodsmentioning

confidence: 99%

“…This system has an edge over the individual cytokine assay using conventional ELISA. Its major strength lies in the fact that it minimises reaction to reaction variations as might be seen while using separate ELISA for each cytokine and can be done in serum samples as low as 25 μL [ 10 , 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Upon reviewing the scientific literature, it is evident that there is an altered expression of inflammatory cytokines and adipokines, such as increased production of pro-inflammatory and decreased production of anti-inflammatory adipo-cytokines in obesity [ 6 , 8 , 9 ]. In our cross-sectional study also, we found that the levels of CRP, IL-8, MCP-1 and TNF-α were higher in individuals with obesity as compared to non-obese [ 10 ]. Pro-inflammatory adipokines like IL-6 and 8, TNF-α and CRP have been found to be involved in increasing vascular reactivity, thrombosis, angiogenesis, insulin resistance, sympathetic nervous activity and inflammation, while anti-inflammatory adipokine like adiponectin were have been found to be protective against obesity and related complications [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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An immediate post op and follow up assessment of circulating adipo-cytokines after bariatric surgery in morbid obesity

et al. 2022

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Background Bariatric surgery has emerged as a promising treatment for improving adipose tissue dysfunction in obesity, but the mechanisms for such amelioration are still not known. This study comprehensively explores a panel of adipo-cytokines in individuals with obesity undergoing bariatric surgery, in conjunction with markers of insulin resistance, at three time points i.e., pre-op, immediate post-op and 6 months post-surgery. Methods It is a case-control prospective study among obese individuals undergoing bariatric surgery (BMI ≥35 kg/m2, n=30) and non-obese subjects (BMI <25 kg/m2, n=30), comparing the levels of serum adiponectin, resistin, C-Reactive Protein (CRP), Interleukin (IL)-6 and 8, Monocyte chemoattractant protein (MCP)-1 and Tumor necrosis factor (TNF)-α between them. The same were followed at immediate and 6-month post-op periods in the former group. The serum markers were correlated with the markers of Insulin resistance like HOMA-IR, HOMA-β and QUICKI. Results A significant increase in adiponectin was seen after weight loss in obese group (17.54 ± 1.31 μg/mL at baseline vs 68.76 ± 1.84 μg/mL at 6- month post-surgery). CRP being an acute phase protein showed significant higher levels at immediate post-op period but declined even below its baseline at 6 months after surgery (33.34 ± 16.85 μg/mL at baseline vs 59.85 ± 23.12 μg/mL at immediate post-op vs 9.66 ± 1.84 μg/mL at 6 months post-operatively). Few inconsistencies were observed in the trajectories of IL-6 and TNF-α, while other pro-inflammatory markers indicated resolution after surgery. Conclusion Bariatric surgery alleviated the systemic inflammation, correlating with improved insulin resistance in individuals with obesity.

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Section: Methodssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An immediate post op and follow up assessment of circulating adipo-cytokines after bariatric surgery in morbid obesity

et al. 2022

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“…In addition, there were also significant between-group differences in correlation coefficients between the serum adiponectin levels and the [ 11 C]PK11195 BP ND values in other ROIs including the bilateral prefrontal, anterior cingulate, and temporal cortices, consistently indicating negative correlations in the MDD group and positive correlations in the control groups. In our study, we used BMI as a covariate since BMI had a weak inverse relationship with blood adiponectin levels [ 64 ] and TSPO binding [ 19 ]. In any event, BMI cannot be a factor in our study, since BMI was matched between the groups (23.6 ± 4.1 vs. 23.6 ± 3.3, p = 0.998).…”

Section: Discussionmentioning

confidence: 99%

In Vivo Cerebral Translocator Protein (TSPO) Binding and Its Relationship with Blood Adiponectin Levels in Treatment-Naïve Young Adults with Major Depression: A [11C]PK11195 PET Study

et al. 2021

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Adiponectin is an adipokine that mediates cellular cholesterol efflux and plays important roles in neuroinflammatory processes. In this study, we undertook positron emission tomography (PET) with the translocator protein (TSPO) ligand [11C]PK11195 and measured serum adiponectin levels in groups of treatment-naïve young adult patients with major depressive disorder (MDD) and matched healthy controls. Thirty treatment-naïve MDD patients (median age: 24 years) and twenty-three healthy controls underwent [11C]PK11195 PET. We quantified TSPO availability in brain as the [11C]PK11195 binding potential (BPND) using a reference tissue model in conjunction with the supervised cluster analysis (SVCA4) algorithm. Age, sex distribution, body mass index, and serum adiponectin levels did not differ between the groups. Between-group analysis using a region-of-interest approach showed significantly higher [11C]PK11195 BPND in the left anterior and right posterior cingulate cortices in MDD patients than in controls. Serum adiponectin levels had significant negative correlations with [11C]PK11195 BPND in the bilateral hippocampus in MDD patients, but significant positive correlations in the bilateral hippocampus in the control group. Our results indicate significantly higher TSPO binding in the anterior and posterior cingulate cortices in treatment-naïve young MDD patients, suggesting microglial activation in these limbic regions, which are involved in cognitive and emotional processing. The opposite correlations between [11C]PK11195 BPND in the hippocampus with serum adiponectin levels in MDD and control groups suggest that microglial activation in the hippocampus may respond differentially to adiponectin signaling in MDD and healthy subjects, possibly with respect to microglial phenotype.

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Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR‐19b‐3p/SOCS3/STAT3 axis mediated autophagy

Yang,

Tan,

et al. 2024

IUBMB Life

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The purpose of this study was to analyze the mechanism by which irisin affects β‐cell pyroptosis in type 2 diabetes mellitus (T2DM). The in vivo T2DM model was established by raised with high‐fat diet and intraperitoneally injection of streptozocin. Min6 cells were divided into four groups: negative control (NC), high glucose (HG), HG + irisin, and HG + irisin+3‐MA. The cell viability was determined by CCK‐8 assay. Dual‐luciferase gene reporter assay was conducted to confirm the binding between miR‐19b‐3p and SOCS3. The expression level of FNDC5 and GSDMD was visualized using the immunofluorescence assay. The protein level of FNDC5, Beclin1, LC3II/I, NLRP3, cleaved‐caspase‐1, GSDMD‐N, STAT3, p‐STAT3, and SOCS3 was determined by Western blotting. The secretion of irisin, lactate dehydrogenase (LDH), and insulin was checked by ELISA. In vivo results showed that pathological changes in islet tissues with declined number of β cells, elevated FBG value, decreased FIN and HOMA‐β value, elevated autophagy‐associated proteins expressions, and activated NLRP3 signaling in T2DM mice, which were dramatically reversed by FNDC5 overexpression. Furthermore, the declined level of miR‐19b‐3p and p‐STAT3, as well as the upregulation of SOCS3, was greatly rescued by FNDC5 overexpression. The in vitro data confirmed the binding site between SOCS3 and miR‐19b‐3p. SOCS3 was downregulated and p‐STAT3 was upregulated in miR‐19b‐3p mimic‐treated Min6 cells. In HG‐stimulated Min6 cells, the elevated cell viability, increased production of insulin, decreased release of LDH, and inactivated NLRP3 signaling induced by irisin were abolished by miR‐19b‐3p inhibitor and STAT3 inhibitor. The increased level of autophagy‐related proteins and activated SOCS3/STAT3 axis induced by irisin in HG‐stimulated Min6 cells were abolished by miR‐19b‐3p inhibitor. The inhibitory effect of irisin against NLRP3 signaling in HG‐stimulated Min6 cells was abrogated by 3‐MA. In conclusion, irisin alleviated the pyroptosis of β cells in T2DM by inhibiting NLRP3 signaling through miR‐19b‐3p/SOCS3/STAT3 axis mediated autophagy.

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Serum Adipocytokines Levels and Their Association with Insulin Sensitivity in Morbidly Obese Individuals Undergoing Bariatric Surgery

Cited by 8 publications

References 37 publications

An immediate post op and follow up assessment of circulating adipo-cytokines after bariatric surgery in morbid obesity

An immediate post op and follow up assessment of circulating adipo-cytokines after bariatric surgery in morbid obesity

In Vivo Cerebral Translocator Protein (TSPO) Binding and Its Relationship with Blood Adiponectin Levels in Treatment-Naïve Young Adults with Major Depression: A [11C]PK11195 PET Study

Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR‐19b‐3p/SOCS3/STAT3 axis mediated autophagy

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