Serum alanine assay with an enzymic micromethod (L-alanine dehydrogenase)

Berg, Aloys; Gerok, W.; Keul, J.

doi:10.1016/0009-8981(78)90463-1

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1979

2019

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Cited by 4 publications

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“…enzymic methods based either on A-alanine-2-oxoglutarate-aminotransferase (EC 2.6.1.2) coupled to lactate dehydrogenase (EC 1.1.1.27) (10,15) or on L-alanine-NAD+oxidoreductase, also called T-alanine dehydrogenase (3,22,24,25). In these enzymic techniques, the deamina tion of Z,-alanine is followed spectrophotometrically at 340 nm or spectrofluorimetrically at 420 nm.…”

Section: Introductionmentioning

confidence: 99%

Semiautomated Enzymic Microassay for Plasma L-Alanine

Stahl¹,

Frick²,

Imler³

et al. 1979

Enzyme

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Plasma L-alanine is deaminated by bacterial alanine-dehydrogenase; the resulting ammonia is dialyzed out and measured by use of a continuous flow phenol-hypochlorite colorimetric microassay. Concentrations in the range of 10 -1,000 μmol alanine/l can be determined in a 50-μl sample. The optimal conditions for the assay are specified. Study of the analytical qualities of the technique shows high specificity, good reproducibility, and a detection limit of 6 μmol/l. Usual values in human plasma from arterial or venous blood are respectively 296 ± 166 and 376 ± 214 ;umol alanine/1 (x ± 2 SD). The usual values in rats are close to those found in man.

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Section: Introductionmentioning

confidence: 99%