Serum aminotransferase activity as a predictor of clearance of drugs metabolized by CYP isoforms in rats with acute hepatic failure induced by carbon tetrachloride

Yokogawa, Koiçhi; Watanabe, Motoko; Takeshita, Harunori; Nomura, Masatoshi; Mano, Yasunari; Miyamoto, Ken Ichi

doi:10.1016/j.ijpharm.2003.09.045

Cited by 37 publications

(39 citation statements)

References 26 publications

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“…The MTT assay was commonly used to define as quantitation of living cells still mitochondrially active in cultures (Brown et al 1994). Also, LDH released into the medium provides an index of cell death and membrane permeability to LDH and an increase in LDH activity in the medium occurs as a result of cell membrane disintegration and enzyme leakage (Yokogawa et al 2004). Applied all methods are often preferred in cytotoxicity studies.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Aydın¹,

Türkez²,

Hacımüftüoğlu³

2017

AKU-J. Sci. Eng.

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Silicon nanoparticles are widely used for various applications including environmental, biological, chemical and physical. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly silicon (as silicon carbide, silicon dioxide, silicon nitride) nanoparticles in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these nanoparticles. HPAEpiC and HPPC cells were treated with silicon (0-100 µg/mL), nanoparticles for 72 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array -RT-PCR assay. According to the results of MTT and LDH assays, all tested nanoparticles induced cytotoxicity on both HPAEpiC and HPPC cells in dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, silicon nanoparticles showed changes in genes related to apoptosis, DNA damage or repair and oxidative stress. This study of gene expression profiles affected by nanotoxicity provides critical information for the clinical and environmental applications of silicon nanoparticles. Silikon Nanopartikül Maruziyetine Bağlı Olarak Oluşan İn Vitro Sitotoksik ve Moleküler Etkiler Anahtar kelimelerAlveolar epitel hücreleri; Genotoksisite; In vitro gen ekspresyonu; Nanotoksisite; Silikon nanopartikülleri ÖzetSilikon nanopartikülleri çevresel, biyolojik, kimyasal ve fiziksel amaçlarla çeşitli alanlarda yaygın olarak kullanılmaktadır. Bu nanopartiküllerin klinik ve endüstriyel alanlarda kullanılabilmesi için güvenilirlikleri özellikle genotoksisite ve sitotoksisite açısından doğrulanmalıdır. Bu yüzden mevcut çalışmada, yaygın olarak kullanılan silikon nanopartiküllerinin ( silikon karbid, silikon dioksit, silikon nitrit) insan alveolar epitel (HPAEpiC) ve farinks (HPPC) hücrelerindeki sitotoksisitesi ve gen ekspresyon profillerindeki değişimlerin araştırılması amaçlanmıştır. HPAEpiC ve HPPC hücreleri 72 saat boyunca silikon nanopartikülleriyle (0-100 µg/mL) muamele edildi. Nanopartiküllerin sitotoksisite değerlendirmeleri için 3-(4,5 dimetylthiazol -2-yl) -2,5 diphenltetrazolium bromide (MTT) ve laktat dehidrogenaz salınım (LDH) yöntemleri kullanılırken; genotoksisite analizi için cDNA array -RT-PCR yöntemi kullanıldı. MTT ve LDH yöntemi sonuçlarına göre, uygulanan bütün test nanopartikülleri hem HPAEpiC hem de HPPC hücre hatlarında doza bağlı olarak sitotoksisiteyi indüklemiştir. HPAEpiC ve HPPC hücrelerinin ilgili genler açısından (apoptozis, DNA fasarı ve tamiri, oksidatif stres) gen ekspresyon profilleri incelendiğinde silikon naopartiküllerinin ekspresyonu değiştirdiği gözlenmiştir. Bu çalışmadan elde edilen nanotoksisiteye bağlı olarak ol...

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Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Aydın¹,

Türkez²,

Hacımüftüoğlu³

2017

AKU-J. Sci. Eng.

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“…The product size was estimated by comparison with a 100 bp DNA ladder initial denaturation at 94 8C for 3 min, repeated denaturation at 94 8C for 45 s, followed with annealing at 66 8C for 45 s for CYP2E1 and 67 8C for 45 s for b-actin, primer extension at 72 8C for 45 s, and final extension at 72 8C for 3 min. The other conditions for RT-PCR were as described previously [17].…”

Section: 6mentioning

confidence: 99%

“…Tissue microsomes were prepared according to Yokogawa et al [17] with slight modifications. Liver, kidney or abdominal fat was homogenized with phosphate buffer (50 mM K 2 HPO 4 containing 0.1 mM EDTA, pH 7.4).…”

Section: Preparation Of Microsomesmentioning

confidence: 99%

Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats

Khemawoot

Yokogawa

Shimada

et al. 2007

Biochemical Pharmacology

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“…Cell death leads to a rise in the concentration of the LDH enzyme in serum and tissues. LDH released into the medium provides an index of cell death and membrane permeability to LDH and an increase in LDH activity in the medium occurs as a result of cell membrane disintegration and enzyme leakage (Yokogawa et al 2004). In addition, cytotoxicity, the degree to which a chemical can cause cell damage, is assessed in this study by the means of MTT assay.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and oxidative damage potentials in human lymphocytes after exposure to terpinolene in vitro

et al. 2014

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Terpinolene (TPO) is a monocyclic monoterpene found in the essential oils of various fir and pine species. Recent reports indicated that several monoterpenes could exhibit antioxidant effects in both human and animal experimental models. However, so far, the nature and/or biological roles of TPO have not been elucidated in human models yet. The aim of this study was to investigate the genetic, oxidative and cytotoxic effects of TPO in cultured human blood cells (n = 5) for the first time. Human blood cells were treated with TPO (0-200 mg/L) for 24 and 48 h, and then cytotoxicity was detected by lactate dehydrogenase (LDH) release and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, while DNA damage was also analyzed by micronucleus assay, sister chromatid exchanges assay and 8-oxo-2-deoxyguanosine (8-OH-dG) level. In addition, biochemical parameters [total antioxidant capacity (TAC) and total oxidative stress (TOS)] were examined to determine oxidative effects. The results of LDH and MTT assays showed that TPO (at concentrations greater than 100 mg/L) decreased cell viability. In our in vitro test systems, it was observed that TPO had no genotoxicity on human lymphocytes. Again, TPO (at 10, 25, 50 and 75 mg/L) treatment caused statistically important (p \ 0.05) increases of TAC levels in human lymphocytes without changing TOS levels. In conclusion, TPO can be a new resource of therapeutics as recognized in this study with its non-genotoxic and antioxidant features.

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Serum aminotransferase activity as a predictor of clearance of drugs metabolized by CYP isoforms in rats with acute hepatic failure induced by carbon tetrachloride

Cited by 37 publications

References 26 publications

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

In Vitro Cytotoxicity and Molecular Effects Related to Silicon Nanoparticles Exposures

Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats

Genotoxic and oxidative damage potentials in human lymphocytes after exposure to terpinolene in vitro

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