Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells

Li, Ying-Bo; Wang, Rui; Wu, Hongli; Li, Yuhua; Zhong, Liang; Yu, He-Ming; Li, Xuejun

doi:10.3892/or_00000041

Cited by 6 publications

(2 citation statements)

References 4 publications

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“…The identified triterpenes have adaptogenic, antihypertensive, and anti-allergic effects. Furthermore, they can inhibit tumor invasion by reducing the expression of metalloproteinases [25] and decreasing tumor metastasis by limiting attachment to endothelial cells [26]. Some polysaccharides present in Reishi, such as beta-glucans, have demonstrated anticancer and immunostimulant activities [27].…”

Section: Mechanism Of Actionmentioning

confidence: 99%

"Medicinal Mushrooms, an Emerging Resource for Human Well-Being in Supplementation and Nutrition. A Special Focus on Ganoderma Lucidum and Agaricus Subrufescens"

Passerini

2023

BJSTR

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Health professionals often know mushrooms mainly from their toxicological aspect. Throughout history, however, mushrooms have occupied an inseparable part of the diet in many countries and are considered a rich source of phytonutrients such as polysaccharides, dietary fiber and other micronutrients, as well as proteins of high biological value. In general, mushrooms offer a wide range of health benefits with a broad spectrum of pharmacological properties, including antitumoral, antidiabetic, antioxidant, antiviral, antibacterial, osteoprotective, nephroprotective, hepatoprotective, etc. In fact, mushrooms are used in the production of nutraceuticals and pharmaceuticals to act as key ingredient in the so-called mycotherapy. This review aims to provide a quick overview of the food and medicinal aspects of mushrooms with an in-depth look at the two most commonly used mushrooms, Ganoderma lucidum and Agaricus subrufescens.

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Section: Mechanism Of Actionmentioning

confidence: 99%

"Medicinal Mushrooms, an Emerging Resource for Human Well-Being in Supplementation and Nutrition. A Special Focus on Ganoderma Lucidum and Agaricus Subrufescens"

Passerini

2023

BJSTR

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“…The AC and DC lens proteins were dissolved in rehydration buffer to a final volume of 150 μl and used for 2-DE as previously described (Li et al, 2008). Firstdimension isoelectric focusing (IEF) involved the use of a 7-cm, pH 3-10, linear-immobilized pH-gradient gel (IPG) strips (Bio-Rad, Hercules, CA).…”

Section: Two-dimensional Gel Electrophoresis (2-de)mentioning

confidence: 99%

Differential Protein Expression between Type 1 Diabetic Cataract and Age-Related Cataract Patients

Qianqian,

Yong,

Zhaodong

et al. 2015

Fol. Biol.

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Diabetes has become one of the major diseases affecting human health. Diabetic cataracts (DCs) are considered a common complication in diabetic patients. The present study investigated differences in lens proteomic profiles between DCs and age-related cataracts (ACs) to determine the mechanism underlying the formation of DCs. Intrasurgical samples were collected from eight DC patients and 12 AC patients, and lens proteins were extracted by lysis and separated using two-dimensional gel electrophoresis (2-DE). The electrophoretic bands were analysed using PD-Quest software 8.0.1. Differentially expressed proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprinting combined with protein database searching. In the 2-DE maps, the DC and AC lens proteins migrated in the region of pH 5–9 with a relative molecular weight (RMW) of 14–97 kDa, whereas the RMW of more abundant crystallin was 20–31 kDa. Approximately three protein spots with differential intensity were detected. Two crystallin proteins (αB and βB1) were identified using MALDI-TOF-MS. Proteomic analysis of the crystalline humour is feasible, and the proteins can be well separated; moreover, differentially expressed lens proteins can be analysed using 2-DE and mass spectrometry to compare DC and AC. The present results indicate that the αB and βB1 crystallins may accelerate the development of DCs. These techniques offer new avenues for mechanistic evaluation and future prevention or therapy of DCs.

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<p>Effect and Mechanism of <em>Ganoderma lucidum</em> Spores on Alleviation of Diabetic Cardiomyopathy in a Pilot in vivo Study</p>

Shaher¹,

Wang²,

Qiu³

et al. 2020

DMSO

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Background Ganoderma lucidum spores (GLS) exhibit disease prevention properties, but no study has been carried out on the anti-diabetic cardiomyopathy property of GLS. The aim of this study was to evaluate the hyperglycemia-mediated cardiomyopathy protection and mechanisms of GLS in streptozotocin (STZ)induced diabetic rats. Methods Male SD rats were randomly divided into three groups. Two groups were given STZ (50 mg/kg, i.p.) treatment and when their fasting plasma glucose was above 16.7 mmol/L, among them, one group was given placebo, as diabetic group, and another group was given GLS (300 mg/kg) treatment. The group without STZ treatment was given placebo as a control group. The experiment lasted 70 days. The histology of myocardium and biomarkers of antioxidants, myocardial injury, pro-inflammatory cytokines, pro-apoptotic proteins and phosphorylation of key proteins in PI3K/AKT pathway were assessed. Results Biochemical analysis showed that GLS treatment significantly reduced the blood glucose (−20.3%) and triglyceride (−20.4%) levels compared to diabetic group without treatment. GLS treatment decreased the content of MDA (−25.6%) and activity of lactate dehydrogenase (−18.9%) but increased the activity of GSH-Px (65.4%). Western blot analysis showed that GLS treatment reduced the expression of both alpha-smooth muscle actin and brain natriuretic peptide. Histological analysis on the cardiac tissue micrographs showed that GLS treatment reduced collagen fibrosis and glycogen reactivity in myocardium. Both Western blot and immunohistochemistry analyses showed that GLS treatment decreased the expression levels of pro-inflammatory factors (cytokines IL-1β, and TNF-α) as well as apoptosis regulatory proteins (Bax, caspase-3 and −9), but increased Bcl-2. Moreover, GLS treatment significantly increased the phosphorylation of key proteins involved in PI3K/AKT pathway, eg, p-AKT p-PI3K and mTOR. Conclusion The results indicated that GLS treatment alleviates diabetic cardiomyopathy by reducing hyperglycemia, oxidative stress, inflammation, apoptosis and further attenuating the fibrosis and myocardial dysfunction induced by STZ through stimulation of the PI3K/Akt/mTOR signaling pathway.

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Serum amyloid A mediates the inhibitory effect of Ganoderma lucidum polysaccharides on tumor cell adhesion to endothelial cells

Cited by 6 publications

References 4 publications

"Medicinal Mushrooms, an Emerging Resource for Human Well-Being in Supplementation and Nutrition. A Special Focus on Ganoderma Lucidum and Agaricus Subrufescens"

"Medicinal Mushrooms, an Emerging Resource for Human Well-Being in Supplementation and Nutrition. A Special Focus on Ganoderma Lucidum and Agaricus Subrufescens"

Differential Protein Expression between Type 1 Diabetic Cataract and Age-Related Cataract Patients

<p>Effect and Mechanism of <em>Ganoderma lucidum</em> Spores on Alleviation of Diabetic Cardiomyopathy in a Pilot in vivo Study</p>

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