Serum amyloid A protein is elevated in relapsing–remitting multiple sclerosis

Ristori, Giovanni; Laurenti, F; Stacchini, Paolo; Gasperini, Claudio; Buttinelli, Carla; Pozzilli, Carlo; Salvetti, Marco

doi:10.1016/s0165-5728(98)00037-x

Cited by 29 publications

(17 citation statements)

References 21 publications

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“…In earlier studies it was shown to be elevated in the serum of RR multiple sclerosis patients [21], and also a transient increase of SAA was observed after intramuscular interferon-β 1a administration [22]. …”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

Füvesi

Hanrieder

Bencsik

et al. 2012

IJMS

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Multiple Sclerosis (MS) is a chronic disease, but in rare fulminant cases rapid progression may lead to death shortly after diagnosis. Currently there is no diagnostic test to predict disease course. The aim of this study was to identify potential biomarkers/proteins related to rapid progression. We present the case history of a 15-year-old male MS patient. Cerebrospinal fluid (CSF) was taken at diagnosis and at the time of rapid progression leading to the patient’s death. Using isobaric tag labeling and nanoflow liquid chromatography in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry we quantitatively analyzed the protein content of two CSF samples from the patient with fulminant MS as well as one relapsing-remitting (RR) MS patient and one control headache patient, whose CSF analysis was normal. Seventy-eight proteins were identified and seven proteins were found to be more abundant in both fulminant MS samples but not in the RR MS sample compared to the control. These proteins are involved in the immune response, blood coagulation, cell proliferation and cell adhesion. In conclusion, in this pilot study we were able to show differences in the CSF proteome of a rapidly progressing MS patient compared to a more typical clinical form of MS and a control subject.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

Füvesi

Hanrieder

Bencsik

et al. 2012

IJMS

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“…Moreover, several transcription factors controlling MHC expression in multiple sclerosis lesions, such as STAT1 and IRF1 (Gobin et al, 2001), were also highly elevated in our sick mice (+15.2 and +10.3, respectively). Strong activation of the immune system in the target organ was suggested by the strong upregulation of genes for MHC Class I and II expression, β‐2 microglobulin, complement components C3 (C3; +11.4), thought to play a pivotal role in the degeneration of oligodendrocytes (Nataf et al, 2000), C1q (3 C1q subunits; from +8.2–+12.2), which contributes to the development of neurological diseases including EAE (Dietzschold et al, 1995), and serum amyloid A3 (SAA, +126.5), a bioactive protein produced during inflammation and found in to be elevated in relapsing‐remitting MS patients (Ristori et al 1998; Boylan et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

CNS gene expression pattern associated with spontaneous experimental autoimmune encephalomyelitis

Matejuk

Hopke

Dwyer

et al. 2003

J of Neuroscience Research

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Transgenic mice with T-cell receptor (TCR) specific for myelin basic protein (MBP)-Ac1-11 peptide and homozygous for the RAG-1 mutation (T/R- mice) spontaneously develop acute progressive experimental autoimmune encephalomyelitis (Sp-EAE) mediated by CD4+ T cells. Microarray analysis of spinal cord tissue obtained from symptomatic versus non-symptomatic T/R- mice revealed strongly upregulated transcripts for genes involved in antigen presentation and processing, signal transduction, transcription regulation, metabolism, development, cell cycle, and many other processes involved in the induction of clinical and pathological signs of Sp-EAE. Several highly expressed genes were related directly to inflammation, including cytokines/receptors, chemokines/receptors, acute phase, complement molecules, and others. Many CNS-specific genes were also upregulated in sick mice. Abundance of message for the Tg TCR BV8S2 gene as well as several monocyte/macrophage-associated genes would suggest that both components play a crucial role in the pathogenesis of Sp-EAE. The profile of transcriptional changes found during the development of Sp-EAE provides the first description of the encephalitogenic process in the absence of purposeful immunization with myelin peptides and immune-enhancing adjuvants. This unique approach is the first to implicate molecules and pathways that contribute naturally to onset of paralysis and demyelination, and thus may provide unique insights and novel treatment strategies for human diseases such as multiple sclerosis.

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“…SAA association with lipid metabolism has been confirmed in multiple studies that investigate the role of SAA in coronary artery disease (CAD) and production of atherosclerotic plaques in blood vessels (Xie et al, 2010, Fyfe et al, 1997, Johnson et al, 2004). The systematic effects of the increased SAA concentration in circulation are also induced by several chronic inflammatory conditions, such as rheumatoid arthritis, multiple sclerosis, type-2 diabetes and some types of cancer (Cunnane, 2001, Artl et al, 2000, Zhao et al, 2010, Ristori et al, 1998). In addition, immune-related and anti-inflammatory roles of SAA have been reported (Badolato et al, 1994, Aldo-Benson and Benson, 1982, Eklund et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of quantitative mass spectrometric immunoassay for serum amyloid A

et al. 2016

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Objective Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states. Methods We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms. Results Intra- and inter-day precision revealed CVs<10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman-Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples, revealed 7 diverse SAA polymorphic types and 12 different proteoforms. Conclusion The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.

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Serum amyloid A protein is elevated in relapsing–remitting multiple sclerosis

Cited by 29 publications

References 21 publications

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

Proteomic Analysis of Cerebrospinal Fluid in a Fulminant Case of Multiple Sclerosis

CNS gene expression pattern associated with spontaneous experimental autoimmune encephalomyelitis

Development of quantitative mass spectrometric immunoassay for serum amyloid A

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