Serum and cerebral spinal fluid levels of chemokines and Th2 cytokines in <i>Schistosoma mansoni</i> myeloradiculopathy

Sousa-Pereira, Sílvio Roberto; Teixeira, Antônio Lúcio; Silva, L. C. S.; Souza, Adriano Lopes de; Antunes, Carlos; Teixeira, Mauro Martins; Lambertucci, José Roberto

doi:10.1111/j.1365-3024.2006.00896.x

Cited by 29 publications

(27 citation statements)

References 20 publications

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“…The main possible weakness was the use of a convenience sample, which makes homogeneity among the selected groups very difficult to obtain, since the levels of proteinuria, the presence and previous use of immunosuppressive drugs and the stage of disease activity at the time of collection may interfere with cytokine and chemokine measurements. Nevertheless, some aspects of the study may increase the strength of our findings, such as the utilization of strictly defined inclusion and exclusion criteria and a well-established protocol for the measurements of cytokines and chemokines with very low intra-assay variability (24).…”

Section: Discussionmentioning

confidence: 99%

“…Plasma and urinary levels of MCP-1/CCL2 and IL-8/CXCL8, plasma TGF-␤ 1 and urinary RANTES/CCL5 were measured by specific enzyme-linked immunoassay (ELISA) kits (R&D Systems, Minneapolis, MN), following the manufacturer's instructions, as described elsewhere (24). Urine chemokine levels were standardized to urine creatinine measured in the same spot urine and expressed as pg/mg cr.…”

Section: Methodsmentioning

confidence: 99%

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Immune Mediators in Idiopathic Nephrotic Syndrome: Evidence for a Relation Between Interleukin 8 and Proteinuria

et al. 2008

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ABSTRACT:The pathogenesis of idiopathic nephrotic syndrome (INS) remains unknown. Several findings suggest a role for the immune system. This study aimed to evaluate immune mediators in INS by measuring plasma and urinary levels of transforming growth factor ␤ 1 (TGF-␤ 1 ), monocyte chemoattractant protein-1 (MCP-1/ CCL2), regulated on activation normal T-cell expressed and secreted (RANTES/CCL5) and IL-8 (IL-8/CXCL8) in pediatric patients with INS and in age-matched healthy controls. Patients were divided according to their response to corticosteroids: steroid-sensitive (SS, n ϭ 8), or steroid-resistant (SR, n ϭ 24). Immune mediators were also compared in regard with disease activity (relapse and remission). Immune mediators were measured by ELISA. Plasma TGF-␤ 1 levels in SR patients were approximately 2.8-fold higher than control values (p Ͻ 0.05). Urinary IL-8/CXCL8 was 2.9-fold higher in INS patients in relapse (proteinuria Ͼ100 mg/m 2 /24 h) when compared with patients in remission (p Ͻ 0.05), and levels had a positive correlation with individual proteinuria values (p Ͻ 0.05). Urinary IL-8/CXCL8 was significantly higher in relapsed SR than in SS patients in remission. No changes in MCP-1/CCL2 and RANTES/CCL5 levels were detected. Our findings suggest that IL-8/CXCL8 and TGF-␤ 1 are involved in the pathogenesis of INS: IL-8/CXCL8 associated with local changes in glomerular permeability and TGF-␤ 1 could be related to worse response to corticosteroids. (Pediatr Res 64: 637-642, 2008)

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Immune Mediators in Idiopathic Nephrotic Syndrome: Evidence for a Relation Between Interleukin 8 and Proteinuria

et al. 2008

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“…For chemokine analysis, plasma samples were thawed and excess proteins, mainly albumin, were removed by acid ⁄salt precipitation (Sousa-Pereira et al 2006). Briefly, an equal volume of plasma and 1.2% trifluoroacetic acid ⁄1.35 M NaCl were mixed and left at room temperature for 10 min.…”

Section: Treatment Of Chc Virus Infection -mentioning

confidence: 99%

Soluble inflammatory markers as predictors of virological response in patients with chronic hepatitis C virus infection treated with interferon-α plus ribavirin

Moura

Carmo

Teixeira

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…However, in the case of our patient, it is difficult to choose an explanation for the spinal cord involvement. No biopsy of the spinal cord to confirm the presence of Schistosoma mansoni eggs 30 was available and hence associated vasculitis cannot be ruled out 33 . It is worth mentioning that there was extensive involvement of the spinal cord in the case presented here (the lesion extended from T1 to T11, as documented by magnetic resonance imaging), which was followed by severe spinal cord atrophy 25 .…”

Section: Discussionmentioning

confidence: 99%

Simultaneous occurrence of brain tumor and myeloradiculopathy in schistosomiasis mansoni: case report

Lambertucci

Souza-Pereira

Carvalho

2009

Rev. Soc. Bras. Med. Trop.

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Simultaneous occurrence of brain tumor and myeloradiculopathy in cases of Manson's schistosomiasis have only rarely been described. We report the case of a 38-year-old man who developed seizures during a trip to Puerto Rico and in whom a brain tumor was diagnosed by magnetic resonance imaging: brain biopsy revealed the diagnosis of schistosomiasis. He was transferred to a hospital in the United States and, during hospitalization, he developed sudden paraplegia. The diagnosis of myeloradiculopathy was confirmed at that time. He was administered praziquantel and steroids. The brain tumor disappeared, but the patient was left with paraplegia and fecal and urinary dysfunction. He has now been followed up in Brazil for one year, and his clinical state, imaging examinations and laboratory tests are presented here. Key-words:Brain tumor. Neuroschistosomiasis. Myeloradiculopathy. RESUMOTem sido descrita, raramente, na esquistossomose mansônica, a ocorrência simultânea de tumor cerebral e mielorradiculopatia. Relatamos aqui o caso de um homem de 38 anos que desenvolveu convulsões, durante viagem a Porto Rico, e um tumor cerebral foi diagnosticado à ressonância magnética: a biópsia do cérebro revelou o diagnóstico de esquistossomose. Ele foi transferido para hospital na América do Norte e durante a hospitalização desenvolveu súbita paraplegia. O diagnóstico de mielorradiculopatia foi confirmado na ocasião. Ele recebeu praziquantel e esteróides. O tumor cerebral desapareceu, mas o paciente permaneceu com paraplegia, disfunção urinária e fecal. Ele tem sido acompanhado no Brasil no último ano e o seu estado clínico, os métodos de imagem e os exames de laboratório são apresentados aqui. Palavras-chaves:Tumor cerebral. Neuroesquistossomose. Mielorradiculopatia. Neurological involvement is associated with infection by the three main species of Schistosoma (Schistosoma mansoni, Schistosoma japonicum and Schistosoma haematobium) 1 2 9 11 13 21 . Although myeloradiculopathy is the most common neurological complication of Schistosoma mansoni infection, the prevalence of cerebral and spinal cord schistosomiasis is unknown. Neuroschistosomiasis is probably underdiagnosed, underestimated and underreported 8 12 14 .Severe neurological involvement has been described during the early post-infective stage in non-immune patients 10 15 . Acute encephalitis and cerebral vasculitis have been reported during primary infection with Schistosoma mansoni 9 28 . Asymptomatic neuroschistosomiasis appears to be more frequent in association with the more severe chronic forms of Schistosoma mansoni. A Brazilian autopsy case series revealed scattered ova of Schistosoma mansoni in 25% of the brains of patients (without neurological deficit) who died due to hepatosplenic schistosomiasis 24 .Schistosoma mansoni can also cause a brain tumor-like syndrome due to slowly expanding cerebral and cerebellar lesions 4 6 15 18 23 24 26 27 .Schistosomal myeloradiculopathy is the most severe and disabling ectopic form of Schistosoma mansoni infection 11 13 1...

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Serum and cerebral spinal fluid levels of chemokines and Th2 cytokines in Schistosoma mansoni myeloradiculopathy

Cited by 29 publications

References 20 publications

Immune Mediators in Idiopathic Nephrotic Syndrome: Evidence for a Relation Between Interleukin 8 and Proteinuria

Immune Mediators in Idiopathic Nephrotic Syndrome: Evidence for a Relation Between Interleukin 8 and Proteinuria

Soluble inflammatory markers as predictors of virological response in patients with chronic hepatitis C virus infection treated with interferon-α plus ribavirin

Simultaneous occurrence of brain tumor and myeloradiculopathy in schistosomiasis mansoni: case report

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