Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children

Marti, Amelia; Morell‐Azanza, Lydia; Rendo-Urteaga, Tara; García-Calzón, Sonia; Ojeda‐Rodríguez, Ana; Martín‐Calvo, Nerea; Moreno-Aliaga, María J.; Martínez, J. Alfredo; Azcona, Cristina

doi:10.1111/pedi.12561

Cited by 31 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results show that those children who had a late dinner had 1.4-fold CRP and 1.6-fold IL-6 values than early eaters. These two pro-inflammatory biomarkers have been related to BMI and have been involved in the pathogenesis of obesity [34,35], which suggests a deleterious association of late eating with inflammatory markers at these ages. Interleukine-6 (IL-6) is the main regulator of the acute inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Late Eating Is Associated with Obesity, Inflammatory Markers and Circadian-Related Disturbances in School-Aged Children

Martínez-Lozano

Tvarijonaviciute

Ríos³

et al. 2020

Nutrients

View full text Add to dashboard Cite

Late eating has been shown to promote metabolic dysregulation and to be associated with obesity in adults. However, few studies have explored this association in children. We compared the presence of obesity, metabolic alterations and circadian-related disturbances between school-aged children who were early dinner eaters (EDE) or late dinner eaters (LDE). School-age children (n = 397; 8–12 years; mean BMI (range): 19.4 kg/m2 (11.6–35.1); 39% overweight/obesity) from Spain were classified into EDE and LDE, according to dinner timing (Median: 21:07). Seven-day-dietary-records were used to assess food-timing and composition. Non-invasive tools were used to collect metabolic biomarkers (saliva), sleep and circadian-related variables (body-temperature and actigraphy). Compared to EDE, LDE were less likely to be overweight/obese [OR: 2.1 (CI: 1.33, 3.31); p = 0.002], and had higher waist-circumference and inflammatory markers, such as IL-6 (1.6-fold) (p = 0.036)) and CRP (1.4-fold) than EDE (p = 0.009). LDE had alterations in the daily patterns of: (a) body-temperature, with a phase delay of 26 min (p = 0.002), and a reduced amplitude (LDE = 0.028 (0.001) and EDE = 0.030 (0.001) (Mean (SEM); p = 0.039); (b) cortisol, with a reduced amplitude (LDE = 0.94 (0.02) and EDE = 1.00 (0.02); p = 0.035). This study represents a significant step towards the understanding of novel aspects in the timing of food intake in children.

show abstract

Section: Discussionmentioning

confidence: 99%

Late Eating Is Associated with Obesity, Inflammatory Markers and Circadian-Related Disturbances in School-Aged Children

Martínez-Lozano

Tvarijonaviciute

Ríos³

et al. 2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…After 4 weeks, blood samples were collected via ocular sampling for isolated serum by clot 2 h at the room temperature and were then centrifuged at 1100 × g for 15 min as previously described. [26] Then liver tissue and colon content were collected and transferred to liquid nitrogen. Serum was collected to analyze LPS, blood glucose, TC, TG, high-density lipoprotein cholesterol (HDL-c), LDL-c, pancreas tissue for gene expression of phosphoinosmde-3-kinase (PI3K), protein kinase B (Akt), IL-2, IL-6, TNF-α, TGF-β, and contents of colon for gut microbiota.…”

Section: Sample Collection and Measurementmentioning

confidence: 99%

Stachyose Improves Inflammation through Modulating Gut Microbiota of High‐Fat Diet/Streptozotocin‐Induced Type 2 Diabetes in Rats

Liu

Jia

Liang

et al. 2018

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…Also, they observed a positive association between CT-1 and arterial damage (increase intima-media thickness). In addition, in obese children, CT-1 transcript levels were reduced after lifestyle interference [80]. Furthermore, Anik Ilhan et al [81] demonstrated that CT-1 levels were found to be positively correlated with DBP and triglyceride levels in MS women with polycystic ovaries.…”

Section: Discussionmentioning

confidence: 99%

N-acetyl Cysteine Can Blunt Metabolic and Cardiovascular Effects via Down-regulation of Cardiotrophin-1 in Rat Model of Fructose-induced Metabolic Syndrome

Abdelhaffez

El-Aziz

Tohamy

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Chronic fructose consumption is associated with development of obesity, insulin resistance (IR) and metabolic syndrome (MS). Cardiovascular diseases are linked to metabolic deregulation observed in MS. N-acetylcysteine (NAC) is a sulfur containing compound of Allium plants (such as garlic and onion) that increases intracellular reduced glutathione concentrations, which is an endogenous antioxidant. Methods This study investigated the ability of N-acetyl cysteine (NAC) to alleviate the metabolic disorders in fructose-induced MS in male Wistar rats and to examine its protective effect on aortic and cardiac tissues via its influence on cardiotrophin-1 (CT-1) expression. NAC (20 mg/kg b.w./day) was administered to fructose (20% w/v) induced MS animals for twelve weeks. Results Chronic fructose consumption increased body weight gain, relative heart weight, systolic blood pressure (SBP), diastolic blood pressure (DBP), IR and associated with metabolic alterations. Histological and immunohistochemical examination revealed aortic stiffness and myocardial degeneration and fibrosis together with increased CT-1 expression. Treatment with NAC improved IR, SBP, DBP and mitigated atherogenic dyslipidaemia and oxidative stress (OS). Additionally, NAC down-regulated CT-1 expression in the heart and aorta. Furthermore, CT-1 expression in the heart and aorta was positively correlated with basal glycemia, final SBP and DBP, total cholesterol, triglycerides, low density lipoproteins and negatively correlated with high density lipoproteins levels. Conclusion The findings reported here demonstrated, for the first time, the protective effect of NAC against aortic and myocardial degeneration and fibrosis through down-regulation of CT-1 in fructose induced MS animal model. Also, our results revealed the infallible role of NAC to blunt the cardiometabolic deregulation observed in MS.

show abstract

Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children

Abstract: We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children. More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.

Cited by 31 publications

References 30 publications

Late Eating Is Associated with Obesity, Inflammatory Markers and Circadian-Related Disturbances in School-Aged Children

Late Eating Is Associated with Obesity, Inflammatory Markers and Circadian-Related Disturbances in School-Aged Children

Stachyose Improves Inflammation through Modulating Gut Microbiota of High‐Fat Diet/Streptozotocin‐Induced Type 2 Diabetes in Rats

N-acetyl Cysteine Can Blunt Metabolic and Cardiovascular Effects via Down-regulation of Cardiotrophin-1 in Rat Model of Fructose-induced Metabolic Syndrome

Contact Info

Product

Resources

About