Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls

Kumar, Pradeep; Suman, .; Pawaria, Shikha; Dalal, Jasmer; Bhardwaj, Sonam; Patil, Sunil Kumar; Adnot, Jérôme; Sharma, Rakesh

doi:10.1016/j.anireprosci.2019.03.010

Cited by 25 publications

(27 citation statements)

References 39 publications

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“…These results indicate that IGF‐I in seminal plasma can be useful for breeders scoring system, such as the testicular IGF‐I has high and positive correlation with sperm quality (Nakayama et al., 1999). In buffalo bulls, the serum IGF‐I was related to sperm concentration and motility, and IGF‐I at 250 ng/ml added to semen improved the motility, membrane integrity and sperm longevity post‐cryopreservation only in fertile, but it not in sub‐fertile bulls (Kumar et al., 2019). Moreover, the authors also found higher serum IGF‐I concentration in fertile than sub‐fertile males (Kumar et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

Souza¹,

Martins

Emerick

et al. 2021

Reprod Domestic Animals

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This study aimed to evaluate the correlation of seminal plasma IGF‐I and total proteins from peripubertal bulls with semen characteristics and onset of puberty. Sixteen dairy Gyr bulls were separated into early (n = 8) and regular (n = 8) groups, according to the onset of puberty. Semen was monthly collected by electroejaculation from 14 to 26 months of age, and the onset of puberty was retrospectively determined (17.0 ± 1.6 and 19.2 ± 1.5 months, to early and regular group, respectively). Five time points were evaluated (day −60, day −30, day 0, day 30 and day 60 days of puberty), being day 0 considered as beginning of puberty. Scrotal circumference and body weight were also assessed. Semen characteristics were evaluated, the seminal plasma was separated by centrifugation and total protein and IGF‐I concentrations were determined. There was no difference between groups to concentration of the seminal plasma total proteins, but we found an interaction between group and age. Seminal plasma IGF‐I concentrations were not different between early and regular groups; thus, the data were combined for analysis. Combined IGF‐I concentrations were positively correlated with sperm motility and concentration, age, body weight and scrotal circumference. Negative correlation was found between IGF‐I concentration and total sperm defects. Increased IGF‐I was observed in day −30 and day 0 of puberty in early and regular groups, respectively. Seminal plasma total proteins are involved in peripubertal modifications and IGF‐I from Zebus dairy bulls can influence the seminal characteristics and the growth factor increase occurs concomitantly with body growth but cannot be used to define puberty bulls earlier.

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Section: Discussionmentioning

confidence: 99%

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

Souza¹,

Martins

Emerick

et al. 2021

Reprod Domestic Animals

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“…After thawing, the contents of the straw (0.21 ml) were transferred to 2 ml tubes maintained in a water bath at 37°C. Sperm motility was subjectively assessed at 0, 30, 60, 90, and 120 min by using a phase‐contrast microscope (Kumar et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Escherichia coli membrane‐derived oxygen‐reducing enzyme system (Oxyrase) protects bubaline spermatozoa during cryopreservation

Dalal

Chandolia

Jan

et al. 2020

Molecular Reproduction Devel

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The objective of this study was to determine the effectiveness of deoxygenation of semen extender using Escherichia coli membrane-derived oxygen scavenger (Oxyrase) on post-thaw quality of buffalo (Bubalus bubalis) spermatozoa. Sixteen semen ejaculates, four each from four bulls, were each divided into five equal fractions, diluted using Tris-egg yolk extender supplemented with different concentrations of Oxyrase (0, 0.3, 0.6, 0.9, and 1.2 U/ml), designated as treatments T1, T2, T3, T4, and T5, respectively, and cryopreserved. Immediately after thawing, Oxyrase did not improve sperm kinetics and motility; however, it improved the keeping quality (significantly lower deterioration of post-thaw sperm motility after incubation for 120 min) in T3. Further, T3 reduced (p < .05) cholesterol efflux and protected the intactness of the sperm plasma membrane. Flow cytometry with Fluo-3 AM/propidium iodide (PI) dual staining revealed the highest (p < .05) proportion of live spermatozoa with low intracellular calcium in T3. Oxyrase supplementation protected spermatozoa from premature capacitation which was confirmed by low expression of tyrosine-phosphorylated proteins (32, 75, and 80 kDa) and a relatively lower percentage of F-pattern (uncapacitated spermatozoa) in chlortetracycline assay. Importantly, the Oxyrase fortification decreased superoxide anion in a dose-dependent manner indicating reduced availability of oxygen at sperm mitochondrial level. Similarly, in Oxyrase-fortified sperm, malondialdehyde concentration, an index of lipid peroxidation, is also reduced in a dose-dependent manner. In conclusion, we demonstrate that deoxygenation of buffalo semen by Oxyrase has the potential of improving post-thaw sperm quality by overcoming the problem of cryocapacitation and oxidative damage during cryopreservation process.

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“…The methanol extracts of extenders were prepared as described by Kumar et al (2019). Briefly, 1‐ml extender was mixed with 10 ml of 80% methanol (80:20, v/v) and pH adjusted to 1.5 with 1‐M HCl.…”

Section: Methodsmentioning

confidence: 99%

Low‐density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action

Dalal

Chandolia

Pawaria

et al. 2020

Molecular Reproduction Devel

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This study was carried out to reveal factors and the mechanism of action by which lowdensity lipoproteins (LDLs) protect sperm better than egg yolk (EY) during cryopreservation. We extracted LDL from EY and compared the amount of calcium, progesterone, and antioxidants in EY and LDL. We found a very high concentration of progesterone (1423.95 vs. 10.46 ng/ml) and calcium (29.19 vs. 0.47 mM) in EY as compared with LDL. Antioxidant assays like DPPH (2,2-diphenyl-1-picrylhydrazyl) and the ferric reducing antioxidants power assay revealed that the LDL extender had almost double ability to lose hydrogen than the EY extender. For sperm cryopreservation, 20 ejaculates from four Murrah buffalo bulls were collected. Each ejaculate was divided into four aliquots and extended in 10%, 12%, and 14% LDL (w/v) and EY-based extenders, followed by cryopreservation. The LDL-based extender prevented excessive cholesterol efflux, and its high content of antioxidants minimized reactive oxygen species generated during cryopreservation, resulting in a functional CatSper channel. The EY-based extender promoted excess cholesterol efflux due to the presence of highdensity lipoprotein, resulting in a compromised CatSper channel. High intracellular calcium in a cryopreserved sperm in the EY group as compared with the LDL group indicates that progesterone present in EY activates the CatSper channel, resulting in a heavy calcium influx into the sperm. The greater tyrosine phosphorylation and increased number of F-pattern in the sperm cryopreserved in the EY extender indicate that high intracellular calcium triggers more capacitation-like changes in the sperm cryopreserved in EY than LDL extender. In conclusion, we demonstrated the new facts and understandings about LDL and EY for semen cryopreservation.

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Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls

Cited by 25 publications

References 39 publications

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

Escherichia coli membrane‐derived oxygen‐reducing enzyme system (Oxyrase) protects bubaline spermatozoa during cryopreservation

Low‐density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action

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